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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Cpb2

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Cpb2 Tafi
Sequence Length (AA) Molecular Weight (Da)
422 48871
Protein Name
Carboxypeptidase B2
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MKLHGLGILV AIILYEQHGF AFQSGQVLSA LPRTSRQVQL LQNLTTTYEV
60 70 80 90 100
VLWQPVTAEF IEKKKEVHFF VNASDVDSVK AHLNVSRIPF NVLMNNVEDL
110 120 130 140 150
IEQQTFNDTV SPRASASYYE QYHSLNEIYS WIEVITEQHP DMLQKIYIGS
160 170 180 190 200
SFEKYPLYVL KVSGKEQRIK NAIWIDCGIH AREWISPAFC LWFIGYVTQF
210 220 230 240 250
HGKENLYTRL LRHVDFYIMP VMNVDGYDYT WKKNRMWRKN RSAHKNNRCV
260 270 280 290 300
GTDLNRNFAS KHWCEKGASS SSCSETYCGL YPESEPEVKA VADFLRRNID
310 320 330 340 350
HIKAYISMHS YSQQILFPYS YNRSKSKDHE ELSLVASEAV RAIESINKNT
360 370 380 390 400
RYTHGSGSES LYLAPGGSDD WIYDLGIKYS FTIELRDTGR YGFLLPERYI
410 420 422
KPTCAEALAA ISKIVWHVIR NT

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-657 Collapse assay details

Data source: Panorama

Official Gene Symbol
CPB2
Peptide Modified Sequence
QVHFFVN[+1.0]ASDVDNVK
Modification Type
Deamidated (NQ)
Protein - Site of Modification
73
Peptide - Site of Modification
7
Peptide Start
67
Peptide End
81
CPTAC ID
CPTAC-657
Peptide Molecular Mass
1,718.8264
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
N/A
Matrix
serum
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Q-Exactive (ThermoFisher)
Internal Standard
synthetic peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Dionex UltiMate 3000 RSLCnano LC (ThermoFisher)
Column Packing
Acclaim PepMap100 C18, 5um (Trap); Acclaim PepMap RSLC C18, 2um (Analytical)
Column Dimensions
300um x 5mm (Trap); 75um x 25cm (Analytical)
Flow Rate
0.5 uL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 10.3 8.2 7.6 10.4 8.8 8.6 14.6 12 11.5 15 15 15
y6 (1+) 6.7 7 10.6 7.2 8.8 8.6 9.8 11.2 13.6 15 15 15
y5 (1+) 8 13.4 15.7 8 14.8 20.1 11.3 20 25.5 15 15 15
sum 6.7 8.3 10.5 7 9.1 12.3 9.7 12.3 16.2 15 15 15

Additional Resources and Comments

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Assay Details for non-CPTAC-5583 Collapse assay details

Data source: Panorama

Official Gene Symbol
Cpb2
Peptide Sequence
DHEELSLVASEAVR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
328
Peptide End
341
CPTAC ID
non-CPTAC-5583
Peptide Molecular Mass
1,553.7686
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b9 (2+) 11.8 7.9 5.2 16.2 8.5 6.7 20 11.6 8.5 15 15 15
b8 (2+) 17.6 10.5 9.3 17.7 12.8 10 25 16.6 13.7 15 15 15
b3 (1+) 25.5 12.8 12.5 24.5 10.5 12.4 35.4 16.6 17.6 15 15 15
y5 (1+) 14.2 3.5 4.6 13.5 7.1 7 19.6 7.9 8.4 15 15 15
y6 (1+) 8.1 4.5 3.4 8 6 7.5 11.4 7.5 8.2 15 15 15
sum 6.7 2.9 2 8.6 5.3 4.7 10.9 6 5.1 15 15 15

Additional Resources and Comments

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Assay Details for non-CPTAC-2639 Collapse assay details

Data source: Panorama

Official Gene Symbol
CPB2
Peptide Sequence
IAWHVIR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
415
Peptide End
421
CPTAC ID
non-CPTAC-2639
Peptide Molecular Mass
893.5236
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 5.4 5 9.9 13 11.8 15.2 14.1 12.8 18.1 15 15 15
y2 (1+) 6.6 5.2 11.5 13.1 7.7 15.1 14.7 9.3 19 15 15 15
y4 (2+) 4.7 6.3 9.8 9.6 10.2 16.6 10.7 12 19.3 15 15 15
y5 (2+) 7.6 4.2 10.7 11 8.6 16.7 13.4 9.6 19.8 15 15 15
b5 (3+) 76.6 40.4 61.8 106.2 53.2 62.9 130.9 66.8 88.2 15 15 15
sum 1.7 4.3 9.5 10.2 9.3 15.1 10.3 10.2 17.8 15 15 15

Additional Resources and Comments

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Comments

Assay Details for non-CPTAC-5582 Collapse assay details

Data source: Panorama

Official Gene Symbol
Cpb2
Peptide Sequence
YGFLLPER
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
391
Peptide End
398
CPTAC ID
non-CPTAC-5582
Peptide Molecular Mass
993.5284
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 3.5 2.2 1.5 5.8 5.8 3.1 6.8 6.2 3.4 15 15 15
b2 (1+) 3.2 2.2 2.3 6.5 5.4 4.2 7.2 5.8 4.8 15 15 15
y4 (1+) 2.4 1.8 2.3 6.6 5.8 4.7 7 6.1 5.2 15 15 15
y5 (1+) 4.4 1.7 0.9 14.1 6 2.6 14.8 6.2 2.8 15 15 15
y7 (1+) 2.6 0.8 2.3 4.9 5.3 3 5.5 5.4 3.8 15 15 15
sum 1 0.6 1.3 6.7 5.4 3.5 6.8 5.4 3.7 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments