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Overview Data source: UniProt

Official Gene Symbol Other Aliases
C8b N/A
Sequence Length (AA) Molecular Weight (Da)
589 66229
Protein Name
Complement component C8 beta chain
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MKIGAQVWRA LAKSCLLCAT LGCLHFPGSR GGKPDFFETK AVNGSLVKSR
60 70 80 90 100
PVRSVAEAPA PIDCELSTWS SWTACDPCQK KRYRHTYLLR PSQFYGELCD
110 120 130 140 150
LSDKEVEDCV TNQPCRSQVR CEGFVCAQTG RCVNRRLLCN GDNDCGDQSD
160 170 180 190 200
EANCRRIYKN CQREMEQYWA IDRLASGINL FTNTFEGPVL DHRYYAGGCS
210 220 230 240 250
PHYILDTNFR KPYNVESYTP QTKCEYEFTL TEYESYSDFE RLVIEKKTHM
260 270 280 290 300
FNFTSGFKVD GVMDLGIKVE SNEGKNYVTR TKRFAHTQSK FLHARSVLEV
310 320 330 340 350
AHYKLKSRSL MLHYEFLQRV KSLPLEYSYG EYRDLLRDFG THFITEAVLG
360 370 380 390 400
GIYEYTLIMN KDAMEQGDYT LSHVTACAGG SFGIGGMVYK VYVKVGVSAK
410 420 430 440 450
KCSDIMKEIN ERNKRSTMVE DLVVLVRGGT SEDITALAYK ELPTPELMEA
460 470 480 490 500
WGDAVKYNPA IIKIKAEPLY ELVTATDFAY SSTVKQNLKK ALEEFQSEVS
510 520 530 540 550
SCRCAPCRGN GVPVLKGSRC ECICPGGFQG TACEVTYRKD IPIDGKWSCW
560 570 580 589
SDWSACSGGH KTRHRQCNNP APHKGGSPCS GPASETLNC

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3535 Collapse assay details

Data source: Panorama

Official Gene Symbol
C8b
Peptide Sequence
GGTSEDITALAYK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
428
Peptide End
440
CPTAC ID
non-CPTAC-3535
Peptide Molecular Mass
1,324.6511
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y2 (1+) 13.7 10 7.8 21 9.8 6.7 25.1 14 10.3 15 15 15
y3 (1+) 9.6 8.2 4.7 15.2 8.6 6.2 18 11.9 7.8 15 15 15
y6 (1+) 9.9 7 6 16.3 9 10.8 19.1 11.4 12.4 15 15 15
y8 (1+) 7.8 4.1 6.8 14.6 6.1 8.4 16.6 7.3 10.8 15 15 15
y9 (1+) 11.5 6.1 3.4 15.1 7.5 10.6 19 9.7 11.1 15 15 15
sum 2.8 2.6 1.4 13.3 5.1 5.7 13.6 5.7 5.9 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-5590 Collapse assay details

Data source: Panorama

Official Gene Symbol
C8b
Peptide Sequence
SVLEVAHYK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
296
Peptide End
304
CPTAC ID
non-CPTAC-5590
Peptide Molecular Mass
1,044.5604
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b7 (2+) 47.8 44.7 56.9 67.3 64.6 71.2 82.5 78.6 91.1 15 15 15
b4 (2+) 12.8 3.3 3.7 16.7 6.4 5 21 7.2 6.2 15 15 15
y5 (3+) 49.7 22.5 47 44.9 27.2 40.5 67 35.3 62 15 15 15
y7 (2+) 5.5 3.4 4.8 9.7 5 5.8 11.2 6 7.5 15 15 15
y5 (1+) 4.9 5.3 4 8.3 8.3 6.7 9.6 9.8 7.8 15 15 15
sum 4.9 2.6 3.2 10.3 5.1 4.8 11.4 5.7 5.8 15 15 15

Additional Resources and Comments