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Overview Data source: UniProt

Official Gene Symbol Other Aliases
C8g N/A
Sequence Length (AA) Molecular Weight (Da)
202 22508
Protein Name
Complement component C8 gamma chain
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MLSPGAVLFF TLLLTASSLG QRTRKPIGST SPISTIQAQV NFSAQKFAGT
60 70 80 90 100
WLLVAVGSSC RFLQEQGHRA EATTLHAAPQ GAAMAVSTFR KLDGICWQVR
110 120 130 140 150
QLFENTGVPG RFLFQVSRAR GPVHMVVAET DYQSFAILYL EQGRKLSVKL
160 170 180 190 200
YVRSLPVNDS VLDVFERRVR EANLTEDQIL FFPKYGFCET ADQLHILNEV
202
PR

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3301 Collapse assay details

Data source: Panorama

Official Gene Symbol
C8g
Peptide Sequence
FLFQVSR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
112
Peptide End
118
CPTAC ID
non-CPTAC-3301
Peptide Molecular Mass
895.4916
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 10.8 6 4.1 16.3 7.2 5.3 19.6 9.4 6.7 15 15 15
y5 (1+) 5.5 3.8 5.6 13.3 5.8 6.4 14.4 6.9 8.5 15 15 15
y3 (1+) 9.3 5.3 4.4 15.2 6.7 6 17.8 8.5 7.4 15 15 15
y4 (1+) 14 3.8 2.8 17.4 4.8 4.5 22.3 6.1 5.3 15 15 15
b2 (1+) 9.5 3.3 3.5 14.1 6.1 4.6 17 6.9 5.8 15 15 15
sum 4.6 2.5 3.4 12.8 5.3 4.4 13.6 5.9 5.6 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-5591 Collapse assay details

Data source: Panorama

Official Gene Symbol
C8g
Peptide Sequence
FLQEQGHR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
62
Peptide End
69
CPTAC ID
non-CPTAC-5591
Peptide Molecular Mass
1,013.5043
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (3+) 38.2 38.5 34.7 39 57.7 40.3 54.6 69.4 53.2 15 15 15
y6 (2+) 3.6 4.8 4.8 7.8 8.7 4.5 8.6 9.9 6.6 15 15 15
y7 (2+) 6.5 5.7 4.2 10.5 6 3.2 12.3 8.3 5.3 15 15 15
y3 (1+) 4.1 5.7 3.8 8.8 8.5 4 9.7 10.2 5.5 15 15 15
y5 (1+) 7.7 6.1 3.5 7.8 8.2 6 11 10.2 6.9 15 15 15
sum 2.2 2.2 1.4 7.7 5.6 1.6 8 6 2.1 15 15 15

Additional Resources and Comments