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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Hrg

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Hrg N/A
Sequence Length (AA) Molecular Weight (Da)
525 59163
Protein Name
Histidine-rich glycoprotein
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MKVLTTALLL VTLQCSHALS PTNCDASEPL AEKVLDLINK GRRSGYVFEL
60 70 80 90 100
LRVSDAHLDR AGTATVYYLA LDVIESDCWV LSTKAQDDCL PSRWQSEIVI
110 120 130 140 150
GQCKVIATRY SNESQDLSVN GYNCTTSSVS SALRNTKDSP VLLDFFEDSE
160 170 180 190 200
LYRKQARKAL DKYKTDNGDF ASFRVERAER VIRARGGERT NYYVEFSMRN
210 220 230 240 250
CSTQHFPRSP LVFGFCRALL SYSIETSDLE TPDSIDINCE VFNIEDHKDT
260 270 280 290 300
SDMKPHWGHE RPLCDKHLCK LSGSRDHHHT HKTDKLGCPP PPEGKDNSDR
310 320 330 340 350
PRLQEGALPQ LPPGYPPHSG ANRTHRPSYN HSCNEHPCHG HRPHGHHPHS
360 370 380 390 400
HHPPGHHSHG HHPHGHHPHS HHSHGHHPPG HHPHGHHPHG HHPHGHHPHG
410 420 430 440 450
HHPHGHDFLD YGPCDPPSNS QELKGQYHRG YGPPHGHSRK RGPGKGLFPF
460 470 480 490 500
HHQQIGYVYR LPPLNIGEVL TLPEANFPSF SLPNCNRSLQ PEIQPFPQTA
510 520 525
SRSCPGKFES EFPQISKFFG YTPPK

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3299 Collapse assay details

Data source: Panorama

Official Gene Symbol
Hrg
Peptide Sequence
FESEFPQISK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
508
Peptide End
517
CPTAC ID
non-CPTAC-3299
Peptide Molecular Mass
1,210.5870
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 10.2 5.2 4.4 16.2 7.3 5.4 19.1 9 7 15 15 15
y6 (1+) 13.9 5.9 4 24.1 12 3.6 27.8 13.4 5.4 15 15 15
y5 (1+) 11.4 7 6.3 14.7 10.8 7.1 18.6 12.9 9.5 15 15 15
y4 (2+) 31.7 14.8 27.3 36.2 19.3 29.3 48.1 24.3 40 15 15 15
b2 (1+) 10.3 6 9 14.6 8.8 11.9 17.9 10.7 14.9 15 15 15
sum 4.4 2.8 4 13.8 7.2 5.7 14.5 7.7 7 15 15 15

Additional Resources and Comments

Resources
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Comments

Assay Details for non-CPTAC-5610 Collapse assay details

Data source: Panorama

Official Gene Symbol
Hrg
Peptide Sequence
SLQPEIQPFPQTASR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
488
Peptide End
502
CPTAC ID
non-CPTAC-5610
Peptide Molecular Mass
1,697.8737
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 12.2 6.2 9.9 16.3 8.4 9.2 20.4 10.4 13.5 15 15 15
b2 (1+) 13.6 7.6 6 19.5 8.7 7.8 23.8 11.6 9.8 15 15 15
y12 (2+) 5.4 6.9 5.4 11 6.2 5.7 12.3 9.3 7.9 15 15 15
y8 (1+) 7.5 4.2 4.4 9.1 8.4 5.1 11.8 9.4 6.7 15 15 15
y9 (1+) 21.9 5.1 7.5 20.7 9.6 8.1 30.1 10.9 11 15 15 15
sum 4.6 3.4 3.3 6 5.9 3.6 7.6 6.8 4.9 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments