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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Itih2

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Itih2 N/A
Sequence Length (AA) Molecular Weight (Da)
946 105928
Protein Name
Inter-alpha-trypsin inhibitor heavy chain H2
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MQRPVCLLIW LFLLEAQAFE IPINGNSEFA EYSDLVELAP DKLPFVQENG
60 70 80 90 100
RHQRSLPEES GEETDTVDPV TLYSYKVQST ITSRVATTTI QSKLVNNSPL
110 120 130 140 150
PQSVVFDVQI PKGAFISNFT MTVNGMTFTS SIKEKTVGRA LYSQARAKGK
160 170 180 190 200
TAGWVRSRTL DMENFNTEVN IPPGAKVQFE LHYQEVKWRK LGSYEHKIHL
210 220 230 240 250
QPGKLAKHLE VNVWIIEPQG MRFLHVPDTF EGHFQGVPVI SKGQQKAHVS
260 270 280 290 300
FKPTVAQQRK CPNCTETAVN GELVVMYDVN REEKAGELEV FNGYFVHFFA
310 320 330 340 350
PENLDPIPKN ILFVIDVSGS MWGIKMKQTV EAMKTILDDL RTDDQFSVVD
360 370 380 390 400
FNHNVRTWRN DLVSATKTQI ADAKRYIEKI QPSGGTNINE ALLRAIFILN
410 420 430 440 450
EASNMGLLNP DSVSLIILVS DGDPTVGELK LSKIQKNVKQ SIQDNISLFS
460 470 480 490 500
LGIGFDVDYD FLKRLSNENR GIAQRIYGNQ DTSSQLKKFY NQVSTPLLRN
510 520 530 540 550
VQFNYPQASV TDVTQNNFHN YFGGSEIVVA GKFDPSKLTE VQSIITATSA
560 570 580 590 600
NTELVLETLS QMDDLEEFLS KDKHADPDFT KKLWAYLTIN QLLAERSLAP
610 620 630 640 650
TAAIKRKITK TILQMSLDHH IVTPLTAMVI ENDAGDERML ADSPPQDHSC
660 670 680 690 700
CSGALYYGTK VASGPIPSWA NPSPTPMSAM LAVGAKPLES TPPTHLNQVE
710 720 730 740 750
NDPHFIIYLP KSKRNICFNI DSEPGKILSL VSDPESGIVV NGQLIGAKRA
760 770 780 790 800
ENGKLSTYFG KLGFYFQKEG MKIEISTETI TLSSGSSTSR LSWSDTAHLG
810 820 830 840 850
NSRVLISVKK EKSVTLTLNK ELFFSVLLHR VWRKHPVNVD FLGIYAPPID
860 870 880 890 900
KFSPRVHGLL GQFMQEPAIH IFNERPGKEP GKPEASMEVK GHKLTVTRGL
910 920 930 940 946
QKDYRTDIVF GTDVPCWFVH NSGKGFIDGH YKDYFVPQLY SFLKRP

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-676 Collapse assay details

Data source: Panorama

Official Gene Symbol
ITIH2
Peptide Modified Sequence
GAFISN[+1.0]FSM[+16.0]TVDGK
Modification Type
Deamidated (NQ), Oxidation (M)
Protein - Site of Modification
118, 121
Peptide - Site of Modification
6, 9
Peptide Start
113
Peptide End
126
CPTAC ID
CPTAC-676
Peptide Molecular Mass
1,489.6759
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
N/A
Matrix
serum
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Q-Exactive (ThermoFisher)
Internal Standard
synthetic peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Dionex UltiMate 3000 RSLCnano LC (ThermoFisher)
Column Packing
Acclaim PepMap100 C18, 5um (Trap); Acclaim PepMap RSLC C18, 2um (Analytical)
Column Dimensions
300um x 5mm (Trap); 75um x 25cm (Analytical)
Flow Rate
0.5 uL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 3.9 3.7 4.7 4.9 4.7 4.8 6.3 6 6.7 15 15 15
y8 (1+) 5.6 5 5.1 6.1 6.6 5.4 8.3 8.3 7.4 15 15 15
y7 (1+) 5 4.4 5.8 5.2 4.7 5.2 7.2 6.4 7.8 15 15 15
sum 3.9 3.2 4.3 4.6 4.2 4.6 6 5.3 6.3 15 15 15

Additional Resources and Comments

Resources
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Assay Details for non-CPTAC-1134 Collapse assay details

Data source: Panorama

Official Gene Symbol
ITIH2
Peptide Sequence
SLAPTAAAK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
597
Peptide End
605
CPTAC ID
non-CPTAC-1134
Peptide Molecular Mass
828.4705
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 11.1 5.2 9.7 14.9 7.7 8.6 18.6 9.3 13 15 15 15
y3 (1+) 12.4 9.4 7.6 22.6 10.9 6.4 25.8 14.4 9.9 15 15 15
y4 (1+) 29 13.7 8 24.8 14.8 11.4 38.2 20.2 13.9 13 15 15
y6 (1+) 10.3 5.6 5.3 12.3 7.8 6.8 16 9.6 8.6 15 15 15
y7 (1+) 16.2 7.2 11.4 19.3 8.3 11.3 25.2 11 16.1 15 15 15
sum 5.3 4.3 4.4 9.2 5.1 4.3 10.6 6.7 6.2 15 15 15

Additional Resources and Comments

Resources
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Comments

Assay Details for non-CPTAC-5611 Collapse assay details

Data source: Panorama

Official Gene Symbol
Itih2
Peptide Sequence
VQFELHYQEVK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
177
Peptide End
187
CPTAC ID
non-CPTAC-5611
Peptide Molecular Mass
1,418.7194
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 12.9 7.4 4.8 16.4 12.8 10.3 20.9 14.8 11.4 15 15 15
y7 (2+) 19.8 4.7 4 20.1 8.1 10.8 28.2 9.4 11.5 15 15 15
y9 (2+) 9.2 4 6.3 9.7 10.6 13 13.4 11.3 14.4 15 15 15
y10 (2+) 20.1 7.7 5.9 23.3 12.8 10.7 30.8 14.9 12.2 15 15 15
y2 (1+) 8.5 7.4 2.6 14 11.8 9.5 16.4 13.9 9.8 15 15 15
sum 6.4 2.8 4.4 8.7 10.3 11.8 10.8 10.7 12.6 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments