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Overview Data source: UniProt

Official Gene Symbol Other Aliases
Tf Trf
Sequence Length (AA) Molecular Weight (Da)
697 76724
Protein Name
Serotransferrin
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MRLTVGALLA CAALGLCLAV PDKTVKWCAV SEHENTKCIS FRDHMKTVLP
60 70 80 90 100
PDGPRLACVK KTSYPDCIKA ISASEADAMT LDGGWVYDAG LTPNNLKPVA
110 120 130 140 150
AEFYGSVEHP QTYYYAVAVV KKGTDFQLNQ LEGKKSCHTG LGRSAGWVIP
160 170 180 190 200
IGLLFCKLSE PRSPLEKAVS SFFSGSCVPC ADPVAFPKLC QLCPGCGCSS
210 220 230 240 250
TQPFFGYVGA FKCLKDGGGD VAFVKHTTIF EVLPEKADRD QYELLCLDNT
260 270 280 290 300
RKPVDQYEDC YLARIPSHAV VARKNNGKED LIWEILKVAQ EHFGKGKSKD
310 320 330 340 350
FQLFSSPLGK DLLFKDSAFG LLRVPPRMDY RLYLGHNYVT AIRNQQEGVC
360 370 380 390 400
PEGSIDNSPV KWCALSHLER TKCDEWSIIS EGKIECESAE TTEDCIEKIV
410 420 430 440 450
NGEADAMTLD GGHAYIAGQC GLVPVMAEYY ESSNCAIPSQ QGIFPKGYYA
460 470 480 490 500
VAVVKASDTS ITWNNLKGKK SCHTGVDRTA GWNIPMGMLY NRINHCKFDE
510 520 530 540 550
FFSQGCAPGY EKNSTLCDLC IGPLKCAPNN KEEYNGYTGA FRCLVEKGDV
560 570 580 590 600
AFVKHQTVLD NTEGKNPAEW AKNLKQEDFE LLCPDGTRKP VKDFASCHLA
610 620 630 640 650
QAPNHVVVSR KEKAARVKAV LTSQETLFGG SDCTGNFCLF KSTTKDLLFR
660 670 680 690 697
DDTKCFVKLP EGTTPEKYLG AEYMQSVGNM RKCSTSRLLE ACTFHKH

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-1157 Collapse assay details

Data source: Panorama

Official Gene Symbol
TF
Peptide Sequence
DGAGDVAFVK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
216
Peptide End
225
CPTAC ID
non-CPTAC-1157
Peptide Molecular Mass
977.4818
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 6 6.6 6 10.8 8.7 6.4 12.4 10.9 8.8 15 15 15
y8 (2+) 8.6 6.6 4.7 12.8 7.2 3.2 15.4 9.8 5.7 15 15 15
y4 (1+) 8.3 5.7 5.7 11.9 9.6 6 14.5 11.2 8.3 15 15 15
y5 (1+) 9.6 6.7 6.6 16 9.8 5.9 18.7 11.9 8.9 15 15 15
y7 (1+) 7.5 5.7 5.6 9.4 9.5 8.1 12 11.1 9.8 15 15 15
sum 3.7 3.9 3.4 8.7 7 4.4 9.5 8 5.6 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-1159 Collapse assay details

Data source: Panorama

Official Gene Symbol
TF
Peptide Sequence
EFQLFSSPHGK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
300
Peptide End
310
CPTAC ID
non-CPTAC-1159
Peptide Molecular Mass
1,275.6248
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (2+) 5.1 4.1 3.9 12.3 5 4 13.3 6.5 5.6 15 15 15
y9 (2+) 7.1 6 4.3 14 9.9 4.8 15.7 11.6 6.4 15 15 15
y3 (1+) 8.8 6 4.8 14.2 10.1 5.1 16.7 11.7 7 15 15 15
y4 (1+) 6 7.2 4.6 11.4 8.8 5 12.9 11.4 6.8 15 15 15
y6 (1+) 5.1 5.6 4.6 11.9 9.3 5.4 12.9 10.9 7.1 15 15 15
sum 3.3 2.8 2.8 11.3 5.3 2.9 11.8 6 4 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-3439 Collapse assay details

Data source: Panorama

Official Gene Symbol
Tf
Peptide Sequence
HQTVLDNTEGK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
555
Peptide End
565
CPTAC ID
non-CPTAC-3439
Peptide Molecular Mass
1,240.6048
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b4 (1+) 5.3 7 6.2 16.9 13.7 9.4 17.7 15.4 11.3 15 15 15
b3 (1+) 6 6.6 8.1 12.9 7.3 9.2 14.2 9.8 12.3 15 15 15
b2 (1+) 5.8 6.6 5.2 14.9 8 7.7 16 10.4 9.3 15 15 15
y2 (1+) 5.5 8.8 9.3 14 9.9 10.2 15 13.2 13.8 15 15 15
y6 (1+) 3.5 8.1 7.8 14.5 7.6 8.2 14.9 11.1 11.3 15 15 15
sum 2.9 3.2 3.2 14.4 7 5.8 14.7 7.7 6.6 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-5621 Collapse assay details

Data source: Panorama

Official Gene Symbol
Tf
Peptide Sequence
IPSHAVVAR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
265
Peptide End
273
CPTAC ID
non-CPTAC-5621
Peptide Molecular Mass
948.5505
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (2+) 7.3 3.8 6.9 9.1 6.1 6.6 11.7 7.2 9.5 15 15 15
y7 (2+) 17 3.3 2.8 17.8 6.8 3.8 24.6 7.6 4.7 15 15 15
y8 (2+) 6.4 2.3 2.3 9.3 6.8 5.9 11.3 7.2 6.3 15 15 15
y4 (1+) 17.2 3.9 4 16.5 7.7 5.1 23.8 8.6 6.5 15 15 15
y5 (1+) 6.5 2.8 4 8.3 5.2 4.5 10.5 5.9 6 15 15 15
sum 3.5 1.4 1.8 5.3 5.5 3.3 6.4 5.7 3.8 15 15 15


Additional Resources and Comments