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Overview Data source: UniProt

Official Gene Symbol Other Aliases
PFN1 N/A
Sequence Length (AA) Molecular Weight (Da)
140 15054
Protein Name
Profilin-1
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAGWNAYIDN LMADGTCQDA AIVGYKDSPS VWAAVPGKTF VNITPAEVGV
60 70 80 90 100
LVGKDRSSFY VNGLTLGGQK CSVIRDSLLQ DGEFSMDLRT KSTGGAPTFN
110 120 130 140
VTVTKTDKTL VLLMGKEGVH GGLINKKCYE MASHLRRSQY

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-253 Collapse assay details

Data source: Panorama

Official Gene Symbol
PFN1
Peptide Sequence
DSPSVWAAVPGK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
27
Peptide End
38
CPTAC ID
CPTAC-253
Peptide Molecular Mass
1,212.6139
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 13.1 12 23 18.2 17.8 28.2 22.4 21.5 36.4 15 25 15
y9 (1+) 23.3 10.1 8.2 25.3 13.8 9.7 34.4 17.1 12.7 15 25 15
y7 (1+) 11.9 7.4 7 13.1 10.6 9.5 17.7 12.9 11.8 15 25 15
sum 9.5 6.8 8.2 10.6 10.3 10.5 14.2 12.3 13.3 15 25 15

Additional Resources and Comments


Assay Details for CPTAC-254 Collapse assay details

Data source: Panorama

Official Gene Symbol
PFN1
Peptide Sequence
TFVNITPAEVGVLVGK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
39
Peptide End
54
CPTAC ID
CPTAC-254
Peptide Molecular Mass
1,642.9294
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y12 (1+) 20.8 9.9 6.2 31.5 17.8 13.2 37.7 20.4 14.6 15 25 15
y11 (1+) 8.6 8.1 5.4 23.9 18.1 14.3 25.4 19.8 15.3 15 25 15
y10 (1+) 13.8 8.4 5.4 27.7 19 14.4 30.9 20.8 15.4 15 25 15
sum 9.3 8.1 5.3 25.2 18.4 14.2 26.9 20.1 15.2 15 25 15

Additional Resources and Comments


Assay Details for non-CPTAC-3933 Collapse assay details

Data source: Panorama

Official Gene Symbol
Pfn1
Peptide Sequence
SSFFVNGLTLGGQK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
57
Peptide End
70
CPTAC ID
non-CPTAC-3933
Peptide Molecular Mass
1,453.7565
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 21.3 4.6 3.6 29.1 9.5 6.8 36.1 10.6 7.7 15 15 15
b4 (1+) 13.5 3.8 5.7 18.3 9 8.7 22.7 9.8 10.4 15 15 15
y10 (1+) 9.4 3.3 2.2 12.5 8.1 5.7 15.6 8.7 6.1 15 15 15
y9 (1+) 12.1 4.1 2.5 15.6 9.6 6.6 19.7 10.4 7.1 15 15 15
y8 (1+) 14.8 4.3 3.7 18.9 6.5 8 24 7.8 8.8 15 15 15
sum 7.2 1.3 2.4 10.1 7.9 6.3 12.4 8 6.7 15 15 15

Additional Resources and Comments