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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
PGLS N/A
Sequence Length (AA) Molecular Weight (Da)
258 27547
Protein Name
6-phosphogluconolactonase
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAAPAPGLIS VFSSSQELGA ALAQLVAQRA ACCLAGARAR FALGLSGGSL
60 70 80 90 100
VSMLARELPA AVAPAGPASL ARWTLGFCDE RLVPFDHAES TYGLYRTHLL
110 120 130 140 150
SRLPIPESQV ITINPELPVE EAAEDYAKKL RQAFQGDSIP VFDLLILGVG
160 170 180 190 200
PDGHTCSLFP DHPLLQEREK IVAPISDSPK PPPQRVTLTL PVLNAARTVI
210 220 230 240 250
FVATGEGKAA VLKRILEDQE ENPLPAALVQ PHTGKLCWFL DEAAARLLTV
258
PFEKHSTL

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3757 Collapse assay details

Data source: Panorama

Official Gene Symbol
Pgls
Peptide Sequence
LLSVPFEK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
246
Peptide End
253
CPTAC ID
non-CPTAC-3757
Peptide Molecular Mass
931.5379
Species
Mus musculus (Mouse)
Assay Type
Direct PRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 5.1 5.9 3.5 7.7 9.7 5.4 9.2 11.4 6.4 15 15 15
y2 (1+) 11 2.7 4.8 14.3 9.3 9.7 18 9.7 10.8 15 15 15
y4 (1+) 6.4 4.6 3.4 7.2 10.2 7.3 9.6 11.2 8.1 15 15 15
y6 (1+) 8.5 2.8 4.6 11.4 9 5.5 14.2 9.4 7.2 15 15 15
y7 (1+) 12.9 7 3.8 11.1 9.9 10 17 12.1 10.7 15 15 15
sum 3.7 2.4 1.1 7.5 8.8 4.8 8.4 9.1 4.9 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-2712 Collapse assay details

Data source: Panorama

Official Gene Symbol
PGLS
Peptide Sequence
VTLTLPVLNAAR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
186
Peptide End
197
CPTAC ID
CPTAC-2712
Peptide Molecular Mass
1,266.7660
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher Q-Exactive
Internal Standard
13 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 um x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 5.8 3.1 6.4 7.6 2.4 6.5 9.6 3.9 9.1 15 15 15
y8 (1+) 13.2 4.1 8 20.6 6.3 8 24.5 7.5 11.3 15 15 15
y9 (1+) 10.6 3.9 7.7 13.3 5.5 8.3 17 6.7 11.3 15 15 15
sum 6.6 3.2 6.6 7.8 2.8 6.8 10.2 4.3 9.5 15 15 15


Additional Resources and Comments