PGLS, CPTAC-2712 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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Overview Data source: UniProt

Official Gene Symbol	Other Aliases
PGLS	N/A

Sequence Length (AA)	Molecular Weight (Da)
258	27547

Protein Name
6-phosphogluconolactonase

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAAPAPGLIS	VFSSSQELGA	ALAQLVAQRA	ACCLAGARAR	FALGLSGGSL
60	70	80	90	100
VSMLARELPA	AVAPAGPASL	ARWTLGFCDE	RLVPFDHAES	TYGLYRTHLL
110	120	130	140	150
SRLPIPESQV	ITINPELPVE	EAAEDYAKKL	RQAFQGDSIP	VFDLLILGVG
160	170	180	190	200
PDGHTCSLFP	DHPLLQEREK	IVAPISDSPK	PPPQRVTLTL	PVLNAARTVI
210	220	230	240	250
FVATGEGKAA	VLKRILEDQE	ENPLPAALVQ	PHTGKLCWFL	DEAAARLLTV
258
PFEKHSTL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-3757 Collapse assay details

Data source: Panorama

Official Gene Symbol: Pgls
Peptide Sequence: LLSVPFEK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 246
Peptide End: 253

CPTAC ID: non-CPTAC-3757
Peptide Molecular Mass: 931.5379
Species: Mus musculus (Mouse)
Assay Type: Direct PRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Publication

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Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >80%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b2 (1+)	5.1	5.9	3.5	7.7	9.7	5.4	9.2	11.4	6.4	15	15	15
y2 (1+)	11	2.7	4.8	14.3	9.3	9.7	18	9.7	10.8	15	15	15
y4 (1+)	6.4	4.6	3.4	7.2	10.2	7.3	9.6	11.2	8.1	15	15	15
y6 (1+)	8.5	2.8	4.6	11.4	9	5.5	14.2	9.4	7.2	15	15	15
y7 (1+)	12.9	7	3.8	11.1	9.9	10	17	12.1	10.7	15	15	15
sum	3.7	2.4	1.1	7.5	8.8	4.8	8.4	9.1	4.9	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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Comments

Assay Details for CPTAC-2712 Collapse assay details

Data source: Panorama

Official Gene Symbol: PGLS
Peptide Sequence: VTLTLPVLNAAR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 186
Peptide End: 197

CPTAC ID: CPTAC-2712
Peptide Molecular Mass: 1,266.7660
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend