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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
GLUD1 GLUD
Sequence Length (AA) Molecular Weight (Da)
558 61398
Protein Name
Glutamate dehydrogenase 1, mitochondrial
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MYRYLGEALL LSRAGPAALG SASADSAALL GWARGQPAAA PQPGLALAAR
60 70 80 90 100
RHYSEAVADR EDDPNFFKMV EGFFDRGASI VEDKLVEDLR TRESEEQKRN
110 120 130 140 150
RVRGILRIIK PCNHVLSLSF PIRRDDGSWE VIEGYRAQHS QHRTPCKGGI
160 170 180 190 200
RYSTDVSVDE VKALASLMTY KCAVVDVPFG GAKAGVKINP KNYTDNELEK
210 220 230 240 250
ITRRFTMELA KKGFIGPGID VPAPDMSTGE REMSWIADTY ASTIGHYDIN
260 270 280 290 300
AHACVTGKPI SQGGIHGRIS ATGRGVFHGI ENFINEASYM SILGMTPGFG
310 320 330 340 350
DKTFVVQGFG NVGLHSMRYL HRFGAKCIAV GESDGSIWNP DGIDPKELED
360 370 380 390 400
FKLQHGSILG FPKAKPYEGS ILEADCDILI PAASEKQLTK SNAPRVKAKI
410 420 430 440 450
IAEGANGPTT PEADKIFLER NIMVIPDLYL NAGGVTVSYF EWLKNLNHVS
460 470 480 490 500
YGRLTFKYER DSNYHLLMSV QESLERKFGK HGGTIPIVPT AEFQDRISGA
510 520 530 540 550
SEKDIVHSGL AYTMERSARQ IMRTAMKYNL GLDLRTAAYV NAIEKVFKVY
558
NEAGVTFT

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3785 Collapse assay details

Data source: Panorama

Official Gene Symbol
Glud1
Peptide Sequence
YNLGLDLR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
528
Peptide End
535
CPTAC ID
non-CPTAC-3785
Peptide Molecular Mass
962.5185
Species
Mus musculus (Mouse)
Assay Type
Direct PRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y2 (1+) 7.5 3 4.4 9.3 8 8.5 11.9 8.5 9.6 15 15 15
y4 (1+) 13.9 6.2 8.4 15.3 12.2 11.6 20.7 13.7 14.3 15 15 15
y5 (1+) 6.4 7.7 6.3 9.4 10 7.1 11.4 12.6 9.5 15 15 15
y6 (1+) 8.3 6.3 8.1 12.5 8.8 9.5 15 10.8 12.5 15 15 15
b2 (1+) 7.3 4.4 6.7 11.5 7.3 7.5 13.6 8.5 10.1 15 15 15
sum 3.9 2.9 2.8 8.4 7.1 5.5 9.3 7.7 6.2 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-2725 Collapse assay details

Data source: Panorama

Official Gene Symbol
GLUD1
Peptide Sequence
HGGTIPIVPTAEFQDR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
481
Peptide End
496
CPTAC ID
CPTAC-2725
Peptide Molecular Mass
1,736.8846
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher Q-Exactive
Internal Standard
13 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N R
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 um x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b5 (1+) 8.1 5.4 6.9 8.1 6.5 7.1 11.5 8.5 9.9 15 15 15
y10 (1+) 151.5 22.4 10.2 194.9 21.9 14.5 246.9 31.3 17.7 15 15 15
y11 (1+) 4.7 5.1 6.7 6.4 6.6 6.7 7.9 8.3 9.5 15 15 15
sum 4.8 4.9 6.6 5.9 6 6.7 7.6 7.7 9.4 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-2726 Collapse assay details

Data source: Panorama

Official Gene Symbol
GLUD1
Peptide Sequence
TAAYVNAIEK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
536
Peptide End
545
CPTAC ID
CPTAC-2726
Peptide Molecular Mass
1,078.5659
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher Q-Exactive
Internal Standard
13 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 um x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 7 5.7 6.9 6.8 5.5 7 9.8 7.9 9.8 15 15 15
y3 (1+) 13.4 7.1 7.5 11.8 8.6 7.8 17.9 11.2 10.8 15 15 15
y4 (1+) 13.7 8.4 8.2 12.8 9.1 10 18.7 12.4 12.9 15 15 15
sum 7 4.2 5.3 6.6 4.1 5.6 9.6 5.9 7.7 15 15 15


Additional Resources and Comments