GLUD1, CPTAC-2726 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
GLUD1	GLUD

Sequence Length (AA)	Molecular Weight (Da)
558	61398

Protein Name
Glutamate dehydrogenase 1, mitochondrial

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MYRYLGEALL	LSRAGPAALG	SASADSAALL	GWARGQPAAA	PQPGLALAAR
60	70	80	90	100
RHYSEAVADR	EDDPNFFKMV	EGFFDRGASI	VEDKLVEDLR	TRESEEQKRN
110	120	130	140	150
RVRGILRIIK	PCNHVLSLSF	PIRRDDGSWE	VIEGYRAQHS	QHRTPCKGGI
160	170	180	190	200
RYSTDVSVDE	VKALASLMTY	KCAVVDVPFG	GAKAGVKINP	KNYTDNELEK
210	220	230	240	250
ITRRFTMELA	KKGFIGPGID	VPAPDMSTGE	REMSWIADTY	ASTIGHYDIN
260	270	280	290	300
AHACVTGKPI	SQGGIHGRIS	ATGRGVFHGI	ENFINEASYM	SILGMTPGFG
310	320	330	340	350
DKTFVVQGFG	NVGLHSMRYL	HRFGAKCIAV	GESDGSIWNP	DGIDPKELED
360	370	380	390	400
FKLQHGSILG	FPKAKPYEGS	ILEADCDILI	PAASEKQLTK	SNAPRVKAKI
410	420	430	440	450
IAEGANGPTT	PEADKIFLER	NIMVIPDLYL	NAGGVTVSYF	EWLKNLNHVS
460	470	480	490	500
YGRLTFKYER	DSNYHLLMSV	QESLERKFGK	HGGTIPIVPT	AEFQDRISGA
510	520	530	540	550
SEKDIVHSGL	AYTMERSARQ	IMRTAMKYNL	GLDLRTAAYV	NAIEKVFKVY
558
NEAGVTFT

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-3785 Collapse assay details

Data source: Panorama

Official Gene Symbol: Glud1
Peptide Sequence: YNLGLDLR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 528
Peptide End: 535

CPTAC ID: non-CPTAC-3785
Peptide Molecular Mass: 962.5185
Species: Mus musculus (Mouse)
Assay Type: Direct PRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Publication

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Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >80%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y2 (1+)	7.5	3	4.4	9.3	8	8.5	11.9	8.5	9.6	15	15	15
y4 (1+)	13.9	6.2	8.4	15.3	12.2	11.6	20.7	13.7	14.3	15	15	15
y5 (1+)	6.4	7.7	6.3	9.4	10	7.1	11.4	12.6	9.5	15	15	15
y6 (1+)	8.3	6.3	8.1	12.5	8.8	9.5	15	10.8	12.5	15	15	15
b2 (1+)	7.3	4.4	6.7	11.5	7.3	7.5	13.6	8.5	10.1	15	15	15
sum	3.9	2.9	2.8	8.4	7.1	5.5	9.3	7.7	6.2	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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Comments

Assay Details for CPTAC-2725 Collapse assay details

Data source: Panorama

Official Gene Symbol: GLUD1
Peptide Sequence: HGGTIPIVPTAEFQDR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 481
Peptide End: 496

CPTAC ID: CPTAC-2725
Peptide Molecular Mass: 1,736.8846
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend