LYN, CPTAC-2785 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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Overview Data source: UniProt

Official Gene Symbol	Other Aliases
LYN	JTK8

Sequence Length (AA)	Molecular Weight (Da)
512	58574

Protein Name
Tyrosine-protein kinase Lyn

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MGCIKSKGKD	SLSDDGVDLK	TQPVRNTERT	IYVRDPTSNK	QQRPVPESQL
60	70	80	90	100
LPGQRFQTKD	PEEQGDIVVA	LYPYDGIHPD	DLSFKKGEKM	KVLEEHGEWW
110	120	130	140	150
KAKSLLTKKE	GFIPSNYVAK	LNTLETEEWF	FKDITRKDAE	RQLLAPGNSA
160	170	180	190	200
GAFLIRESET	LKGSFSLSVR	DFDPVHGDVI	KHYKIRSLDN	GGYYISPRIT
210	220	230	240	250
FPCISDMIKH	YQKQADGLCR	RLEKACISPK	PQKPWDKDAW	EIPRESIKLV
260	270	280	290	300
KRLGAGQFGE	VWMGYYNNST	KVAVKTLKPG	TMSVQAFLEE	ANLMKTLQHD
310	320	330	340	350
KLVRLYAVVT	REEPIYIITE	YMAKGSLLDF	LKSDEGGKVL	LPKLIDFSAQ
360	370	380	390	400
IAEGMAYIER	KNYIHRDLRA	ANVLVSESLM	CKIADFGLAR	VIEDNEYTAR
410	420	430	440	450
EGAKFPIKWT	APEAINFGCF	TIKSDVWSFG	ILLYEIVTYG	KIPYPGRTNA
460	470	480	490	500
DVMTALSQGY	RMPRVENCPD	ELYDIMKMCW	KEKAEERPTF	DYLQSVLDDF
510	512
YTATEGQYQQ	QP

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-2785 Collapse assay details

Data source: Panorama

Official Gene Symbol: LYN
Peptide Modified Sequence: TIY[+80.0]VRDPTSNK
Modification Type: Phospho (Y)
Protein - Site of Modification: N/A
Peptide - Site of Modification: 3
Peptide Start: 30
Peptide End: 40

CPTAC ID: CPTAC-2785
Peptide Molecular Mass: 1,372.6388
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: Mammalian Cell Lysate
Submitting Laboratory: Johns Hopkins University / Pandey
Submitting Lab PI: Akhilesh Pandey

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Orbitrap Fusion Lumos
Internal Standard: 100fmol
Peptide Standard Purity: Crude (~60%)
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Easy nLC 1200 System
Column Packing: C18 2um 100A
Column Dimensions: 75um i.d. x 50cm
Flow Rate: 250nl/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y7 (1+)	5.4	15.3	16.3	5.4	16.6	15.9	7.6	22.6	22.8	15	15	15
b4 (1+)	15.6	19.4	11.9	15.8	18.5	12.6	22.2	26.8	17.3	15	15	15
b7 (1+)	19.3	24.9	27.6	17.8	24	25.2	26.3	34.6	37.4	15	15	15
b9 (2+)	15.6	19.4	11.9	15.8	18.5	12.6	22.2	26.8	17.3	15	15	15
sum	6.6	16.5	15.5	6	16.2	14.9	8.9	23.1	21.5	15	15	15

LYN

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-2785 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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LYN

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-2785 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments