MELK, CPTAC-2888 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
MELK	KIAA0175

Sequence Length (AA)	Molecular Weight (Da)
651	74642

Protein Name
Maternal embryonic leucine zipper kinase

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MKDYDELLKY	YELHETIGTG	GFAKVKLACH	ILTGEMVAIK	IMDKNTLGSD
60	70	80	90	100
LPRIKTEIEA	LKNLRHQHIC	QLYHVLETAN	KIFMVLEYCP	GGELFDYIIS
110	120	130	140	150
QDRLSEEETR	VVFRQIVSAV	AYVHSQGYAH	RDLKPENLLF	DEYHKLKLID
160	170	180	190	200
FGLCAKPKGN	KDYHLQTCCG	SLAYAAPELI	QGKSYLGSEA	DVWSMGILLY
210	220	230	240	250
VLMCGFLPFD	DDNVMALYKK	IMRGKYDVPK	WLSPSSILLL	QQMLQVDPKK
260	270	280	290	300
RISMKNLLNH	PWIMQDYNYP	VEWQSKNPFI	HLDDDCVTEL	SVHHRNNRQT
310	320	330	340	350
MEDLISLWQY	DHLTATYLLL	LAKKARGKPV	RLRLSSFSCG	QASATPFTDI
360	370	380	390	400
KSNNWSLEDV	TASDKNYVAG	LIDYDWCEDD	LSTGAATPRT	SQFTKYWTES
410	420	430	440	450
NGVESKSLTP	ALCRTPANKL	KNKENVYTPK	SAVKNEEYFM	FPEPKTPVNK
460	470	480	490	500
NQHKREILTT	PNRYTTPSKA	RNQCLKETPI	KIPVNSTGTD	KLMTGVISPE
510	520	530	540	550
RRCRSVELDL	NQAHMEETPK	RKGAKVFGSL	ERGLDKVITV	LTRSKRKGSA
560	570	580	590	600
RDGPRRLKLH	YNVTTTRLVN	PDQLLNEIMS	ILPKKHVDFV	QKGYTLKCQT
610	620	630	640	650
QSDFGKVTMQ	FELEVCQLQK	PDVVGIRRQR	LKGDAWVYKR	LVEDILSSCK
651
V

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-1169 Collapse assay details

Data source: Panorama

Official Gene Symbol: MELK
Peptide Sequence: DLKPENLLFDEYHK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 132
Peptide End: 145

CPTAC ID: CPTAC-1169
Peptide Molecular Mass: 1,759.8781
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: Ovarian cancer tumor tissue lysate
Submitting Laboratory: Pacific Northwest National Laboratory
Submitting Lab PI: Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TSQ Vantage
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Waters nanoACQUITY (Part Number 176016000)
Column Packing: Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions: 100 um x 100 mm
Flow Rate: 0.5 uL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y5 (1+)	30.4	16.5	12.9	34.3	17.2	15.1	45.8	23.8	19.9	15	15	15
y6 (1+)	10.7	12.2	8.4	10.9	13.2	8.9	15.3	18	12.2	15	15	15
y7 (1+)	27	13.6	10.5	25.5	15.4	12.1	37.1	20.5	16	15	15	15
sum	4.4	6.6	7.9	7.2	7.8	9.2	8.4	10.2	12.1	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for CPTAC-2888 Collapse assay details

Data source: Panorama

Official Gene Symbol: MELK
Peptide Sequence: LHYNVTTTR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 559
Peptide End: 567

CPTAC ID: CPTAC-2888
Peptide Molecular Mass: 1,103.5724
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend