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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
CHUK IKKA, TCF16
Sequence Length (AA) Molecular Weight (Da)
745 84640
Protein Name
Inhibitor of nuclear factor kappa-B kinase subunit alpha
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MERPPGLRPG AGGPWEMRER LGTGGFGNVC LYQHRELDLK IAIKSCRLEL
60 70 80 90 100
STKNRERWCH EIQIMKKLNH ANVVKACDVP EELNILIHDV PLLAMEYCSG
110 120 130 140 150
GDLRKLLNKP ENCCGLKESQ ILSLLSDIGS GIRYLHENKI IHRDLKPENI
160 170 180 190 200
VLQDVGGKII HKIIDLGYAK DVDQGSLCTS FVGTLQYLAP ELFENKPYTA
210 220 230 240 250
TVDYWSFGTM VFECIAGYRP FLHHLQPFTW HEKIKKKDPK CIFACEEMSG
260 270 280 290 300
EVRFSSHLPQ PNSLCSLVVE PMENWLQLML NWDPQQRGGP VDLTLKQPRC
310 320 330 340 350
FVLMDHILNL KIVHILNMTS AKIISFLLPP DESLHSLQSR IERETGINTG
360 370 380 390 400
SQELLSETGI SLDPRKPASQ CVLDGVRGCD SYMVYLFDKS KTVYEGPFAS
410 420 430 440 450
RSLSDCVNYI VQDSKIQLPI IQLRKVWAEA VHYVSGLKED YSRLFQGQRA
460 470 480 490 500
AMLSLLRYNA NLTKMKNTLI SASQQLKAKL EFFHKSIQLD LERYSEQMTY
510 520 530 540 550
GISSEKMLKA WKEMEEKAIH YAEVGVIGYL EDQIMSLHAE IMELQKSPYG
560 570 580 590 600
RRQGDLMESL EQRAIDLYKQ LKHRPSDHSY SDSTEMVKII VHTVQSQDRV
610 620 630 640 650
LKELFGHLSK LLGCKQKIID LLPKVEVALS NIKEADNTVM FMQGKRQKEI
660 670 680 690 700
WHLLKIACTQ SSARSLVGSS LEGAVTPQTS AWLPPTSAEH DHSLSCVVTP
710 720 730 740 745
QDGETSAQMI EENLNCLGHL STIIHEANEE QGNSMMNLDW SWLTE

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1220 Collapse assay details

Data source: Panorama

Official Gene Symbol
CHUK
Peptide Modified Sequence
LGTGGFGNVC[+57.0]LYQHR
Modification Type
Carbamidomethyl Cysteine
Protein - Site of Modification
30
Peptide - Site of Modification
10
Peptide Start
21
Peptide End
35
CPTAC ID
CPTAC-1220
Peptide Molecular Mass
1,677.8046
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 27.1 22.7 10.2 50.8 23.6 15.5 57.6 32.7 18.6 15 15 15
y5 (1+) 31 23.6 16.1 41.9 28.5 26.4 52.1 37 30.9 15 15 15
y6 (1+) 19.3 18.9 22.7 42.3 19.8 26.5 46.5 27.4 34.9 15 15 15
sum 16 18.9 9.1 38.8 20.9 19 42 28.2 21.1 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-1221 Collapse assay details

Data source: Panorama

Official Gene Symbol
CHUK
Peptide Sequence
VWAEAVHYVSGLK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
426
Peptide End
438
CPTAC ID
CPTAC-1221
Peptide Molecular Mass
1,457.7667
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 27.7 10.5 10.3 31.2 19.9 31.3 41.7 22.5 33 15 15 15
y6 (1+) 18.9 6.7 5.3 32.7 19.6 23.5 37.8 20.7 24.1 15 15 15
y7 (1+) 20.2 4.8 5.9 35.2 20.1 25 40.6 20.7 25.7 15 15 15
sum 15.2 4.3 5 32.1 19.3 25.1 35.5 19.8 25.6 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-2837 Collapse assay details

Data source: Panorama

Official Gene Symbol
CHUK
Peptide Sequence
HRPSDHSYSDSTEMVK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
573
Peptide End
588
CPTAC ID
CPTAC-2837
Peptide Molecular Mass
1,874.8217
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive™
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
EASY-nLC™ 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nl/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 12.3 4.2 4.2 10 5.2 4.7 15.9 6.7 6.3 15 15 15
y7 (1+) 14.3 6.3 4.3 14.7 5.4 4.9 20.5 8.3 6.5 15 15 15
y3 (1+) 7.8 3.6 4.5 7.3 4.9 4.7 10.7 6.1 6.5 15 15 15
sum 8 3.9 3.9 7.3 4.4 4.3 10.8 5.9 5.8 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-3087 Collapse assay details

Data source: Panorama

Official Gene Symbol
CHUK
Peptide Modified Sequence
LGTGGFGNVC[+57.0]LYQHR
Modification Type
Carbamidomethyl (C)
Protein - Site of Modification
31
Peptide - Site of Modification
10
Peptide Start
21
Peptide End
35
CPTAC ID
CPTAC-3087
Peptide Molecular Mass
1,677.8046
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 8.9 7.4 8.7 9.3 7.7 8.5 12.9 10.7 12.2 15 15 15
y5 (1+) 11 8.4 7 9.7 9.2 7.6 14.7 12.5 10.3 15 15 15
y4 (1+) 10.8 9.3 7.6 14.2 9.5 7.8 17.8 13.3 10.9 15 15 15
sum 10 8.5 7.3 10.8 8.7 7.7 14.7 12.2 10.6 15 15 15


Additional Resources and Comments