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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

EPHA3

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
EPHA3 ETK, ETK1, HEK, TYRO4
Sequence Length (AA) Molecular Weight (Da)
983 110131
Protein Name
Ephrin type-A receptor 3
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MDCQLSILLL LSCSVLDSFG ELIPQPSNEV NLLDSKTIQG ELGWISYPSH
60 70 80 90 100
GWEEISGVDE HYTPIRTYQV CNVMDHSQNN WLRTNWVPRN SAQKIYVELK
110 120 130 140 150
FTLRDCNSIP LVLGTCKETF NLYYMESDDD HGVKFREHQF TKIDTIAADE
160 170 180 190 200
SFTQMDLGDR ILKLNTEIRE VGPVNKKGFY LAFQDVGACV ALVSVRVYFK
210 220 230 240 250
KCPFTVKNLA MFPDTVPMDS QSLVEVRGSC VNNSKEEDPP RMYCSTEGEW
260 270 280 290 300
LVPIGKCSCN AGYEERGFMC QACRPGFYKA LDGNMKCAKC PPHSSTQEDG
310 320 330 340 350
SMNCRCENNY FRADKDPPSM ACTRPPSSPR NVISNINETS VILDWSWPLD
360 370 380 390 400
TGGRKDVTFN IICKKCGWNI KQCEPCSPNV RFLPRQFGLT NTTVTVTDLL
410 420 430 440 450
AHTNYTFEID AVNGVSELSS PPRQFAAVSI TTNQAAPSPV LTIKKDRTSR
460 470 480 490 500
NSISLSWQEP EHPNGIILDY EVKYYEKQEQ ETSYTILRAR GTNVTISSLK
510 520 530 540 550
PDTIYVFQIR ARTAAGYGTN SRKFEFETSP DSFSISGESS QVVMIAISAA
560 570 580 590 600
VAIILLTVVI YVLIGRFCGY KSKHGADEKR LHFGNGHLKL PGLRTYVDPH
610 620 630 640 650
TYEDPTQAVH EFAKELDATN ISIDKVVGAG EFGEVCSGRL KLPSKKEISV
660 670 680 690 700
AIKTLKVGYT EKQRRDFLGE ASIMGQFDHP NIIRLEGVVT KSKPVMIVTE
710 720 730 740 750
YMENGSLDSF LRKHDAQFTV IQLVGMLRGI ASGMKYLSDM GYVHRDLAAR
760 770 780 790 800
NILINSNLVC KVSDFGLSRV LEDDPEAAYT TRGGKIPIRW TSPEAIAYRK
810 820 830 840 850
FTSASDVWSY GIVLWEVMSY GERPYWEMSN QDVIKAVDEG YRLPPPMDCP
860 870 880 890 900
AALYQLMLDC WQKDRNNRPK FEQIVSILDK LIRNPGSLKI ITSAAARPSN
910 920 930 940 950
LLLDQSNVDI TTFRTTGDWL NGVWTAHCKE IFTGVEYSSC DTIAKISTDD
960 970 980 983
MKKVGVTVVG PQKKIISSIK ALETQSKNGP VPV

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1774 Collapse assay details

Data source: Panorama

Official Gene Symbol
EPHA3
Peptide Modified Sequence
VLEDDPEAAY[+80.0]TTR
Modification Type
Phospho (Y)
Protein - Site of Modification
779
Peptide - Site of Modification
10
Peptide Start
770
Peptide End
782
CPTAC ID
CPTAC-1774
Peptide Molecular Mass
1,558.6552
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
E.coli Lysate
Submitting Laboratory
Johns Hopkins University / Pandey
Submitting Lab PI
Akhilesh Pandey

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Orbitrap Fusion Lumos
Internal Standard
100fmol
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy nLC 1200 System
Column Packing
C18 2um 100A
Column Dimensions
75um i.d. x 50cm
Flow Rate
300nl/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 5.9 6.1 6.1 6 8.1 8.8 8.4 10.1 10.7 15 15 15
y5 (1+) 6.5 7.7 8 6.2 8.8 10.7 9 11.7 13.4 15 15 15
y6 (1+) 5.1 7.4 6.4 5.7 8.4 11.4 7.6 11.2 13.1 15 15 15
y8 (1+) 6.2 8.4 7.5 6.2 9 12.1 8.8 12.3 14.2 15 15 15
y9 (1+) 7.6 9.4 6.4 7.4 10 12.9 10.6 13.7 14.4 15 15 15
y10 (1+) 6.1 7.8 7.8 6.5 8.5 12.4 8.9 11.5 14.6 15 15 15
sum 5.7 7.8 7 5.7 8.6 11.4 8.1 11.6 13.4 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for CPTAC-3203 Collapse assay details

Data source: Panorama

Official Gene Symbol
EPHA3
Peptide Modified Sequence
EIFTGVEYSSC[+57.0]DTIAK
Modification Type
Carbamidomethyl (C)
Protein - Site of Modification
941
Peptide - Site of Modification
11
Peptide Start
930
Peptide End
945
CPTAC ID
CPTAC-3203
Peptide Molecular Mass
1,818.8346
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 13.2 3.7 3.8 13.8 10.3 13.4 19.1 10.9 13.9 15 15 15
y9 (1+) 6.6 3.3 5.1 12.8 9.7 12.2 14.4 10.2 13.2 15 15 15
y10 (1+) 9.5 3.5 5 11.7 10.7 13.6 15.1 11.3 14.5 15 15 15
sum 8.6 3 4.4 11.6 10.1 12.9 14.4 10.5 13.6 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for CPTAC-3202 Collapse assay details

Data source: Panorama

Official Gene Symbol
EPHA3
Peptide Modified Sequence
VVGAGEFGEVC[+57.0]SGR
Modification Type
Carbamidomethyl (C)
Protein - Site of Modification
637
Peptide - Site of Modification
11
Peptide Start
626
Peptide End
639
CPTAC ID
CPTAC-3202
Peptide Molecular Mass
1,422.6562
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y12 (1+) 5.5 2.6 2.7 5.1 4.2 3.5 7.5 4.9 4.4 15 15 15
y8 (1+) 4.2 3.8 2.4 4.3 4.7 2.5 6 6 3.5 15 15 15
y7 (1+) 5.8 2.8 3 5.9 4 3 8.3 4.9 4.2 15 15 15
sum 4.7 2.9 2.3 4.7 4.3 2.9 6.6 5.2 3.7 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments