| Official Gene Symbol | Other Aliases |
|---|---|
| PCNA | N/A |
| Sequence Length (AA) | Molecular Weight (Da) |
|---|---|
| 261 | 28769 |
| Protein Name |
|---|
| Proliferating cell nuclear antigen |
| Sources | |
|---|---|
|
UniProt PhosphoSitePlus ® GeneCards |
Human Protein Atlas |
| 10 | 20 | 30 | 40 | 50 |
| MFEARLVQGS | ILKKVLEALK | DLINEACWDI | SSSGVNLQSM | DSSHVSLVQL |
| 60 | 70 | 80 | 90 | 100 |
| TLRSEGFDTY | RCDRNLAMGV | NLTSMSKILK | CAGNEDIITL | RAEDNADTLA |
| 110 | 120 | 130 | 140 | 150 |
| LVFEAPNQEK | VSDYEMKLMD | LDVEQLGIPE | QEYSCVVKMP | SGEFARICRD |
| 160 | 170 | 180 | 190 | 200 |
| LSHIGDAVVI | SCAKDGVKFS | ASGELGNGNI | KLSQTSNVDK | EEEAVTIEMN |
| 210 | 220 | 230 | 240 | 250 |
| EPVQLTFALR | YLNFFTKATP | LSSTVTLSMS | ADVPLVVEYK | IADMGHLKYY |
| 260 | 261 | |||
| LAPKIEDEEG | S |
Data source: UniProt
Uniprot Database Entry PhosphoSitePlus ®
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Data source: Panorama
View Details (opens in a new window)
A Multiplexed Mass Spectrometry-Based Assay for Robust Quantification of Phosphosignaling in Response to DNA Damage. Whiteaker JR, Zhao L, Saul R, Kaczmarczyk JA, Schoenherr RM, Moore HD, Jones-Weinert C, Ivey RG, Lin C, Hiltke T, Reding KW, Whiteley G, Wang P, Paulovich AG. Radiat Res. 2018 Feb 23. doi: 10.1667/RR14963.1. [Epub ahead of print]
Data source: Panorama
Data source: Panorama
Data source: Panorama
Retrieving Data
Data source: Panorama
| Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y5 (1+) | 39.2 | 16.6 | 20 | 16.1 | 29 | 37.2 | 42.4 | 33.4 | 42.2 | 15 | 15 | 15 |
| y6 (1+) | 42.5 | 19 | 21.2 | 25.2 | 31.4 | 36.5 | 49.4 | 36.7 | 42.2 | 15 | 15 | 15 |
| y8 (1+) | 44.2 | 21.8 | 23.6 | 28 | 33.9 | 34.5 | 52.3 | 40.3 | 41.8 | 15 | 15 | 15 |
| y10 (1+) | 34.9 | 19.3 | 20.3 | 29.9 | 29.5 | 34.7 | 46 | 35.3 | 40.2 | 15 | 15 | 15 |
| y9 (1+) | 32.6 | 22.8 | 18 | 30.6 | 33.8 | 34.9 | 44.7 | 40.8 | 39.3 | 15 | 15 | 15 |
| sum | 36.1 | 17.6 | 19.9 | 23.2 | 31.4 | 34.8 | 42.9 | 36 | 40.1 | 15 | 15 | 15 |
Data source: Panorama
View Details (opens in a new window)
A Multiplexed Mass Spectrometry-Based Assay for Robust Quantification of Phosphosignaling in Response to DNA Damage. Whiteaker JR, Zhao L, Saul R, Kaczmarczyk JA, Schoenherr RM, Moore HD, Jones-Weinert C, Ivey RG, Lin C, Hiltke T, Reding KW, Whiteley G, Wang P, Paulovich AG. Radiat Res. 2018 Feb 23. doi: 10.1667/RR14963.1. [Epub ahead of print]
Data source: Panorama
Data source: Panorama
Data source: Panorama
Retrieving Data
Data source: Panorama
| Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| b9 (1+) | 26.5 | 11.4 | 11.5 | 45.2 | 11.3 | 13.2 | 52.4 | 16.1 | 17.5 | 15 | 15 | 15 |
| y6 (1+) | 21.4 | 13 | 7.3 | 28.2 | 17.2 | 11.4 | 35.4 | 21.6 | 13.5 | 15 | 15 | 15 |
| y7 (1+) | 23.2 | 19.9 | 10.7 | 47.6 | 27.1 | 13.1 | 53 | 33.6 | 16.9 | 15 | 15 | 15 |
| sum | 22.5 | 9.8 | 8.4 | 35.6 | 10.9 | 11 | 42.1 | 14.7 | 13.8 | 15 | 15 | 15 |
Data source: Panorama
Data source: Panorama
Data source: Panorama
Data source: Panorama
Retrieving Data
Data source: Panorama
| Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y5 (1+) | 6.3 | 3.1 | 3.5 | 8.2 | 3.4 | 4.1 | 10.3 | 4.6 | 5.4 | 15 | 15 | 15 |
| y6 (1+) | 7.2 | 3.4 | 2.5 | 8.6 | 3.9 | 4.1 | 11.2 | 5.2 | 4.8 | 15 | 15 | 15 |
| b9 (2+) | 30.2 | 9.9 | 5.6 | 32.8 | 12 | 5.8 | 44.6 | 15.6 | 8.1 | 15 | 15 | 15 |
| sum | 6.4 | 2.7 | 2.5 | 8.2 | 3.2 | 3.6 | 10.4 | 4.2 | 4.4 | 15 | 15 | 15 |
Data source: Panorama
View Details (opens in a new window)
A Multiplexed Mass Spectrometry-Based Assay for Robust Quantification of Phosphosignaling in Response to DNA Damage. Whiteaker JR, Zhao L, Saul R, Kaczmarczyk JA, Schoenherr RM, Moore HD, Jones-Weinert C, Ivey RG, Lin C, Hiltke T, Reding KW, Whiteley G, Wang P, Paulovich AG. Radiat Res. 2018 Feb 23. doi: 10.1667/RR14963.1. [Epub ahead of print]
Data source: Panorama
Data source: Panorama
Data source: Panorama
Retrieving Data
Data source: Panorama
| Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y9 (1+) | 20 | 13.3 | 7.5 | 19.6 | 15.4 | 8.9 | 28 | 20.3 | 11.6 | 15 | 15 | 15 |
| y8 (1+) | 21.2 | 23 | 17.1 | 24.9 | 30.7 | 13.9 | 32.7 | 38.4 | 22 | 15 | 15 | 15 |
| y6 (1+) | 24.3 | 19.1 | 18.5 | 41.9 | 26.3 | 20.4 | 48.4 | 32.5 | 27.5 | 15 | 15 | 15 |
| y7 (1+) | 35.3 | 14.5 | 13.8 | 42.5 | 17.2 | 11.4 | 55.2 | 22.5 | 17.9 | 15 | 15 | 15 |
| b8 (1+) | 39.5 | 10.7 | 13.4 | 41 | 19.6 | 18.5 | 56.9 | 22.3 | 22.8 | 15 | 15 | 15 |
| b9 (2+) | 38.6 | 19.8 | 12.8 | 45.6 | 21 | 16.7 | 59.7 | 28.9 | 21 | 15 | 15 | 15 |
| sum | 12.2 | 13.8 | 7.6 | 15.2 | 15.1 | 8.7 | 19.5 | 20.5 | 11.6 | 15 | 15 | 15 |
Data source: Panorama
Data source: Panorama
Data source: Panorama
Data source: Panorama
Retrieving Data
Data source: Panorama
| Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y9 (1+) | 14.3 | 8.1 | 7.7 | 14 | 6.8 | 10.1 | 20 | 10.6 | 12.7 | 15 | 15 | 15 |
| y8 (1+) | 12.4 | 4.7 | 5.2 | 13 | 5.9 | 8.6 | 18 | 7.5 | 10 | 15 | 15 | 15 |
| y7 (1+) | 10.4 | 4.5 | 9.7 | 11.2 | 10.5 | 11.3 | 15.3 | 11.4 | 14.9 | 15 | 15 | 15 |
| y6 (1+) | 13 | 7 | 14.7 | 10.5 | 5.6 | 13.1 | 16.7 | 9 | 19.7 | 15 | 15 | 15 |
| sum | 10.7 | 4.6 | 6.3 | 11 | 5.6 | 8.5 | 15.3 | 7.2 | 10.6 | 15 | 15 | 15 |
Data source: Panorama
View Details (opens in a new window)
Anti-peptide monoclonal antibodies generated for immuno-multiple reaction monitoring-mass spectrometry assays have a high probability of supporting Western blot and ELISA. Schoenherr RM, Saul RG, Whiteaker JR, Yan P, Whiteley GR, Paulovich AG. Mol Cell Proteomics. 2015 Feb;14(2):382-98. doi: 10.1074/mcp.O114.043133. Epub 2014 Dec 15. PMID: 25512614
Data source: Panorama
Data source: Panorama
Data source: Panorama
Retrieving Data
Data source: Panorama
| Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| b3 (1+) | 26.8 | 8.2 | 3.3 | 23.8 | 8.4 | 5.5 | 35.8 | 11.7 | 6.4 | 15 | 15 | 15 |
| y7 (2+) | 32.6 | 7.8 | 2.3 | 29.5 | 9.5 | 4.2 | 44 | 12.3 | 4.8 | 15 | 15 | 15 |
| y4 (1+) | 43.7 | 16.1 | 5.5 | 47.7 | 17.2 | 5.5 | 64.7 | 23.6 | 7.8 | 15 | 15 | 15 |
| y5 (1+) | 17.2 | 5.1 | 1.4 | 19.1 | 6.7 | 3 | 25.7 | 8.4 | 3.3 | 15 | 15 | 15 |
| sum | 17.6 | 4.7 | 1.1 | 14.2 | 5.5 | 3.6 | 22.6 | 7.2 | 3.8 | 15 | 15 | 15 |
Data source: Panorama
View Details (opens in a new window)
A Multiplexed Mass Spectrometry-Based Assay for Robust Quantification of Phosphosignaling in Response to DNA Damage. Whiteaker JR, Zhao L, Saul R, Kaczmarczyk JA, Schoenherr RM, Moore HD, Jones-Weinert C, Ivey RG, Lin C, Hiltke T, Reding KW, Whiteley G, Wang P, Paulovich AG. Radiat Res. 2018 Feb 23. doi: 10.1667/RR14963.1. [Epub ahead of print]
Data source: Panorama
Data source: Panorama
Data source: Panorama
Retrieving Data
Data source: Panorama
| Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y5 (1+) | 19.4 | 7.4 | 8.3 | 19.8 | 11.3 | 9 | 27.7 | 13.5 | 12.2 | 15 | 15 | 15 |
| y7 (2+) | 47.5 | 10.4 | 14.8 | 31.8 | 14.9 | 14.5 | 57.2 | 18.2 | 20.7 | 15 | 15 | 15 |
| y4 (1+) | 27.4 | 21.5 | 8.9 | 27.8 | 22.1 | 11.5 | 39 | 30.8 | 14.5 | 15 | 15 | 15 |
| sum | 17.3 | 6.8 | 7.1 | 15.4 | 10.4 | 6.3 | 23.2 | 12.4 | 9.5 | 15 | 15 | 15 |
Data source: Panorama
Data source: Panorama
Data source: Panorama
Data source: Panorama
Retrieving Data
Data source: Panorama
| Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y5 (1+) | 4.5 | 2.2 | 4.8 | 11.6 | 9.7 | 8.5 | 12.4 | 9.9 | 9.8 | 15 | 15 | 15 |
| y4 (1+) | 12.8 | 4.4 | 3.6 | 13.9 | 8.4 | 9.2 | 18.9 | 9.5 | 9.9 | 15 | 15 | 15 |
| sum | 3.7 | 2.3 | 4.7 | 11.2 | 9.6 | 8.5 | 11.8 | 9.9 | 9.7 | 15 | 15 | 15 |
Data source: Panorama
Data source: Panorama
Data source: Panorama
Data source: Panorama
Retrieving Data
Data source: Panorama
| Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y4 (1+) | 16.5 | 12.1 | 19.9 | 25.9 | 14.5 | 15.8 | 30.7 | 18.9 | 25.4 | 15 | 15 | 15 |
| y5 (1+) | 13.3 | 11.9 | 15.6 | 13.3 | 10.8 | 14.4 | 18.8 | 16.1 | 21.2 | 15 | 15 | 15 |
| y6 (1+) | 11.5 | 6.2 | 9.6 | 13.6 | 10.5 | 11.6 | 17.8 | 12.2 | 15.1 | 15 | 15 | 15 |
| sum | 11 | 9.3 | 14.2 | 13.9 | 9.2 | 12.2 | 17.7 | 13.1 | 18.7 | 15 | 15 | 15 |
Data source: Panorama
Data source: Panorama
Data source: Panorama
Data source: Panorama
Retrieving Data
Data source: Panorama
| Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y6 (1+) | 12.5 | 7.8 | 8.4 | 17.7 | 8.5 | 10.1 | 21.7 | 11.5 | 13.1 | 15 | 15 | 15 |
| y7 (1+) | 12 | 6.5 | 10.4 | 14.1 | 8.9 | 9.3 | 18.5 | 11 | 14 | 15 | 15 | 15 |
| y10 (1+) | 12.7 | 6.5 | 10.5 | 18 | 10.2 | 9.7 | 22 | 12.1 | 14.3 | 15 | 15 | 15 |
| sum | 6.4 | 6.4 | 9.3 | 13.3 | 8.4 | 9.4 | 14.8 | 10.6 | 13.2 | 15 | 15 | 15 |
Data source: Panorama
View Details (opens in a new window)
Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253
Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.
Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.
Data source: Panorama
Data source: Panorama
Data source: Panorama
Retrieving Data
Data source: Panorama
| Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y7 (1+) | 26.6 | 17.7 | 9.1 | 36 | 19.2 | 13 | 44.8 | 26.1 | 15.9 | 25 | 15 | 15 |
| y6 (1+) | 8.4 | 18.5 | 5.9 | 7.3 | 15 | 6.3 | 11.1 | 23.8 | 8.6 | 25 | 15 | 15 |
| y5 (1+) | 10.6 | 16.8 | 3.4 | 10.6 | 15.7 | 5.2 | 15 | 23 | 6.2 | 25 | 15 | 15 |
| sum | 8.8 | 17.3 | 4.2 | 8.3 | 15.3 | 4.8 | 12.1 | 23.1 | 6.4 | 25 | 15 | 15 |
Data source: Panorama
View Details (opens in a new window)
Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253
Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.
Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.
Data source: Panorama
Data source: Panorama
Data source: Panorama
Retrieving Data
Data source: Panorama
| Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y10 (1+) | 18.7 | 5.6 | 4.7 | 30.8 | 10 | 3.9 | 36 | 11.5 | 6.1 | 15 | 25 | 15 |
| y9 (1+) | 26.8 | 7 | 4.1 | 26.2 | 8.1 | 5.3 | 37.5 | 10.7 | 6.7 | 15 | 25 | 15 |
| y7 (1+) | 31.9 | 5.8 | 4.9 | 37.2 | 6.9 | 6.8 | 49 | 9 | 8.4 | 15 | 25 | 15 |
| sum | 14.6 | 4.1 | 3.9 | 25.7 | 6.6 | 4.3 | 29.6 | 7.8 | 5.8 | 15 | 25 | 15 |
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