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Overview Data source: UniProt

Official Gene Symbol Other Aliases
CLU APOJ, CLI, KUB1
Sequence Length (AA) Molecular Weight (Da)
449 52495
Protein Name
Clusterin
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MMKTLLLFVG LLLTWESGQV LGDQTVSDNE LQEMSNQGSK YVNKEIQNAV
60 70 80 90 100
NGVKQIKTLI EKTNEERKTL LSNLEEAKKK KEDALNETRE SETKLKELPG
110 120 130 140 150
VCNETMMALW EECKPCLKQT CMKFYARVCR SGSGLVGRQL EEFLNQSSPF
160 170 180 190 200
YFWMNGDRID SLLENDRQQT HMLDVMQDHF SRASSIIDEL FQDRFFTREP
210 220 230 240 250
QDTYHYLPFS LPHRRPHFFF PKSRIVRSLM PFSPYEPLNF HAMFQPFLEM
260 270 280 290 300
IHEAQQAMDI HFHSPAFQHP PTEFIREGDD DRTVCREIRH NSTGCLRMKD
310 320 330 340 350
QCDKCREILS VDCSTNNPSQ AKLRRELDES LQVAERLTRK YNELLKSYQW
360 370 380 390 400
KMLNTSSLLE QLNEQFNWVS RLANLTQGED QYYLRVTTVA SHTSDSDVPS
410 420 430 440 449
GVTEVVVKLF DSDPITVTVP VEVSRKNPKF METVAEKALQ EYRKKHREE

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-728 Collapse assay details

Data source: Panorama

Official Gene Symbol
CLU
Peptide Sequence
ASSIIDELFQDR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
183
Peptide End
194
CPTAC ID
CPTAC-728
Peptide Molecular Mass
1,392.6885
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
N/A
Matrix
plasma
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Anti-peptide monoclonal antibodies generated for immuno-multiple reaction monitoring-mass spectrometry assays have a high probability of supporting Western blot and ELISA. Schoenherr RM, Saul RG, Whiteaker JR, Yan P, Whiteley GR, Paulovich AG. Mol Cell Proteomics. 2015 Feb;14(2):382-98. doi: 10.1074/mcp.O114.043133. Epub 2014 Dec 15. PMID: 25512614


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 Qtrap
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Eksigent NanoLC 2D
Column Packing
ReproSil-Pur C18-AQ 3 um
Column Dimensions
100 mm x 75 um ID
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 55.3 23 8.5 53.3 23 23.7 76.8 32.5 25.2 15 15 15
y4 (1+) 41.2 13 9.1 44 22.3 20.4 60.3 25.8 22.3 15 15 15
y6 (1+) 25.7 16.4 14.9 36 12.6 15.9 44.2 20.7 21.8 15 15 15
sum 32.9 7.3 8.3 36.7 13.5 18.8 49.3 15.3 20.6 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-661 Collapse assay details

Data source: Panorama

Official Gene Symbol
CLU
Peptide Modified Sequence
EDALN[+1.0]ETR
Modification Type
Deamidated (NQ)
Protein - Site of Modification
86
Peptide - Site of Modification
5
Peptide Start
82
Peptide End
89
CPTAC ID
CPTAC-661
Peptide Molecular Mass
947.4196
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
N/A
Matrix
serum
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Q-Exactive (ThermoFisher)
Internal Standard
synthetic peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Dionex UltiMate 3000 RSLCnano LC (ThermoFisher)
Column Packing
Acclaim PepMap100 C18, 5um (Trap); Acclaim PepMap RSLC C18, 2um (Analytical)
Column Dimensions
300um x 5mm (Trap); 75um x 25cm (Analytical)
Flow Rate
0.5 uL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 6.2 3.2 3.9 6.9 3.1 4.1 9.3 4.5 5.7 15 15 15
y5 (1+) 4.9 4 3.4 4.7 3.1 3.1 6.8 5.1 4.6 15 15 15
y4 (1+) 3.8 2.6 2.9 3.3 2.3 3.2 5 3.5 4.3 15 15 15
sum 3.5 2.5 2.9 3.4 2.1 2.9 4.9 3.3 4.1 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-662 Collapse assay details

Data source: Panorama

Official Gene Symbol
CLU
Peptide Modified Sequence
LAN[+1.0]LTQGEDQYYLR
Modification Type
Deamidated (NQ)
Protein - Site of Modification
374
Peptide - Site of Modification
3
Peptide Start
372
Peptide End
385
CPTAC ID
CPTAC-662
Peptide Molecular Mass
1,683.8104
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
N/A
Matrix
serum
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Q-Exactive (ThermoFisher)
Internal Standard
synthetic peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Dionex UltiMate 3000 RSLCnano LC (ThermoFisher)
Column Packing
Acclaim PepMap100 C18, 5um (Trap); Acclaim PepMap RSLC C18, 2um (Analytical)
Column Dimensions
300um x 5mm (Trap); 75um x 25cm (Analytical)
Flow Rate
0.5 uL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 5.7 3.3 4.7 6 3.3 5.6 8.3 4.7 7.3 15 15 15
y9 (1+) 7.9 3.7 3.9 6.1 4.5 4.6 10 5.8 6 15 15 15
y8 (1+) 5.6 4.2 5.3 6.2 5.3 5.4 8.4 6.8 7.6 15 15 15
sum 5.1 3.5 4.6 5.7 4.4 5.1 7.6 5.6 6.9 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-478 Collapse assay details

Data source: Panorama

Official Gene Symbol
CLU
Peptide Modified Sequence
EILSVDC[+57.0]STNNPSQAK
Modification Type
Carbamidomethyl Cysteine
Protein - Site of Modification
313
Peptide - Site of Modification
7
Peptide Start
307
Peptide End
322
CPTAC ID
CPTAC-478
Peptide Molecular Mass
1,761.8203
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Seoul National University / Korea Institute of Science and Technology and FHCRC
Submitting Lab PI
Youngsoo Kim

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y11 (1+) 23.7 10.8 6.5 30.1 10.7 7.8 38.3 15.2 10.2 15 25 15
y10 (1+) 24.9 13.7 19.8 27 14.5 22.7 36.7 19.9 30.1 15 25 15
y9 (1+) 34.7 18.1 8.6 38.4 18.1 15.7 51.8 25.6 17.9 15 25 15
sum 20.2 10 8 27.3 10.6 10.4 34 14.6 13.1 15 25 15

Additional Resources and Comments


Assay Details for CPTAC-479 Collapse assay details

Data source: Panorama

Official Gene Symbol
CLU
Peptide Sequence
LFDSDPITVTVPVEVSR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
409
Peptide End
425
CPTAC ID
CPTAC-479
Peptide Molecular Mass
1,872.9833
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Seoul National University / Korea Institute of Science and Technology and FHCRC
Submitting Lab PI
Youngsoo Kim

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 35.8 19.1 15.4 32.7 27.2 26.2 48.5 33.2 30.4 15 15 15
y8 (1+) 31.1 11.3 22.6 44.1 21.2 37.6 54 24 43.9 15 15 15
y7 (1+) 56 22.2 40.2 46.3 32.2 48.5 72.7 39.1 63 15 15 15
sum 17.9 5 9.5 28.8 19.9 15.2 33.9 20.5 17.9 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-1096 Collapse assay details

Data source: Panorama

Official Gene Symbol
CLU
Peptide Sequence
ELDESLQVAER
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
326
Peptide End
336
CPTAC ID
non-CPTAC-1096
Peptide Molecular Mass
1,287.6307
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y2 (1+) 25.7 6.3 6.2 27.2 14.7 6.3 37.4 16 8.8 15 15 15
y3 (1+) 10.2 9.9 7.1 12.6 9.3 7.3 16.2 13.6 10.2 15 15 15
y4 (1+) 15.8 5.6 8.5 15.9 10.2 7.5 22.4 11.6 11.3 15 15 15
y7 (1+) 18.1 6.2 6.4 14.9 14.3 6.8 23.4 15.6 9.3 15 15 15
y9 (1+) 23.6 8.7 8.3 20.7 12.9 9.1 31.4 15.6 12.3 15 15 15
sum 10.6 4.3 4.7 8.3 9.8 5 13.5 10.7 6.9 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-1098 Collapse assay details

Data source: Panorama

Official Gene Symbol
CLU
Peptide Sequence
EPQDTYHYLPFSLPHR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
199
Peptide End
214
CPTAC ID
non-CPTAC-1098
Peptide Molecular Mass
1,998.9588
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y12 (3+) 6.8 5.9 4.4 11.9 6.7 6.3 13.7 8.9 7.7 15 15 15
y13 (3+) 4.8 5.8 3.8 10.5 7.6 7 11.5 9.6 8 15 15 15
y3 (2+) 7.3 6.2 3.7 12.2 7.4 9.7 14.2 9.7 10.4 15 15 15
y7 (2+) 6.6 4.7 4.1 11.8 5.2 6.9 13.5 7 8 15 15 15
y3 (1+) 7.7 4.6 4.3 11.7 5 4.5 14 6.8 6.2 15 15 15
sum 6.2 4.2 3.4 11 5.5 5.2 12.6 6.9 6.2 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-5594 Collapse assay details

Data source: Panorama

Official Gene Symbol
Clu
Peptide Sequence
IDSLLESDR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
158
Peptide End
166
CPTAC ID
non-CPTAC-5594
Peptide Molecular Mass
1,046.5244
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 6.1 5 4.5 7.8 5.3 4.8 9.9 7.3 6.6 15 15 15
y4 (1+) 6.9 7.2 3 18.5 8.3 5.4 19.7 11 6.2 15 15 15
y5 (1+) 7.2 2.6 4.6 13.3 5.9 5.2 15.1 6.4 6.9 15 15 15
y7 (1+) 7.7 3 5.1 7.9 6.4 5.7 11 7.1 7.6 15 15 15
y8 (1+) 6.3 7.9 4.3 9.1 9.2 6.3 11.1 12.1 7.6 15 15 15
sum 4 1.6 2.1 10.1 4.5 3.3 10.9 4.8 3.9 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-5593 Collapse assay details

Data source: Panorama

Official Gene Symbol
Clu
Peptide Sequence
SLLNSLEEAK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
68
Peptide End
77
CPTAC ID
non-CPTAC-5593
Peptide Molecular Mass
1,102.5870
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 18 4.4 4.5 27.5 7.9 6 32.9 9 7.5 15 15 15
y8 (2+) 19.5 4.3 2.5 26.7 8.1 7.1 33.1 9.2 7.5 15 15 15
y6 (1+) 11.8 3.5 4.9 12.2 9 7.1 17 9.7 8.6 15 15 15
y7 (1+) 7.2 3.8 3.8 6.3 6.8 7.9 9.6 7.8 8.8 15 15 15
y8 (1+) 11.8 4.5 5.2 17.7 6.7 7.7 21.3 8.1 9.3 15 15 15
sum 11.7 2 1.6 19.7 6.2 5.9 22.9 6.5 6.1 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-1097 Collapse assay details

Data source: Panorama

Official Gene Symbol
CLU
Peptide Sequence
TLLSNLEEAK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
69
Peptide End
78
CPTAC ID
non-CPTAC-1097
Peptide Molecular Mass
1,116.6027
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 11 5.3 7 19.7 5.7 5.9 22.6 7.8 9.2 14 15 15
b2 (1+) 9 5 4.8 18.3 5.6 4.2 20.4 7.5 6.4 15 15 15
y8 (2+) 18.6 6.4 5.9 27.7 6.8 4.6 33.4 9.3 7.5 15 15 15
y7 (1+) 10.2 5.3 4.7 13.2 8.4 5.8 16.7 9.9 7.5 15 15 15
y8 (1+) 7 7.4 3.3 9.6 7.4 4.9 11.9 10.5 5.9 14 15 15
sum 7.9 4.2 3.6 11.4 5 3.4 13.9 6.5 5 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-5592 Collapse assay details

Data source: Panorama

Official Gene Symbol
Clu
Peptide Sequence
VSTVTTHSSDSEVPSR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
385
Peptide End
400
CPTAC ID
non-CPTAC-5592
Peptide Molecular Mass
1,687.8013
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y12 (2+) 12.3 6.8 6 13.6 8.1 5.3 18.3 10.6 8 15 15 15
y13 (2+) 13 4.3 5.5 15.6 7.9 5.3 20.3 9 7.6 15 15 15
y14 (2+) 3.2 3.6 4.8 6.6 8.6 5.2 7.3 9.3 7.1 15 15 15
y3 (1+) 12.8 5.4 4.8 13.6 8.7 8.4 18.7 10.2 9.7 15 15 15
y9 (1+) 6.9 6.8 5.5 9 8.9 6.1 11.3 11.2 8.2 15 15 15
sum 3.8 1.7 2.4 6.5 7 3.5 7.5 7.2 4.2 15 15 15

Additional Resources and Comments