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Overview Data source: UniProt

Official Gene Symbol Other Aliases
CORO1C CRN2, CRNN4
Sequence Length (AA) Molecular Weight (Da)
474 53249
Protein Name
Coronin-1C
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MRRVVRQSKF RHVFGQAVKN DQCYDDIRVS RVTWDSSFCA VNPRFVAIII
60 70 80 90 100
EASGGGAFLV LPLHKTGRID KSYPTVCGHT GPVLDIDWCP HNDQVIASGS
110 120 130 140 150
EDCTVMVWQI PENGLTLSLT EPVVILEGHS KRVGIVAWHP TARNVLLSAG
160 170 180 190 200
CDNAIIIWNV GTGEALINLD DMHSDMIYNV SWNRNGSLIC TASKDKKVRV
210 220 230 240 250
IDPRKQEIVA EKEKAHEGAR PMRAIFLADG NVFTTGFSRM SERQLALWNP
260 270 280 290 300
KNMQEPIALH EMDTSNGVLL PFYDPDTSII YLCGKGDSSI RYFEITDESP
310 320 330 340 350
YVHYLNTFSS KEPQRGMGYM PKRGLDVNKC EIARFFKLHE RKCEPIIMTV
360 370 380 390 400
PRKSDLFQDD LYPDTAGPEA ALEAEEWFEG KNADPILISL KHGYIPGKNR
410 420 430 440 450
DLKVVKKNIL DSKPTANKKC DLISIPKKTT DTASVQNEAK LDEILKEIKS
460 470 474
IKDTICNQDE RISKLEQQMA KIAA

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-50 Collapse assay details

Data source: Panorama

Official Gene Symbol
CORO1C
Peptide Sequence
AIFLADGNVFTTGFSR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
224
Peptide End
239
CPTAC ID
CPTAC-50
Peptide Molecular Mass
1,714.8679
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y11 (1+) 15.2 11.6 9.8 24.5 19.3 22.7 28.8 22.5 24.7 25 15 15
y10 (1+) 10.9 12.3 12 16.7 18.2 21.4 19.9 22 24.5 25 15 15
y8 (1+) 13.7 20.4 14.3 22.6 30 30 26.4 36.3 33.2 25 15 15
sum 10.6 9 6.1 19.5 17.3 20.3 22.2 19.5 21.2 25 15 15

Additional Resources and Comments


Assay Details for CPTAC-49 Collapse assay details

Data source: Panorama

Official Gene Symbol
CORO1C
Peptide Modified Sequence
VTWDSSFC[+57.0]AVNPR
Modification Type
Carbamidomethyl Cysteine
Protein - Site of Modification
39
Peptide - Site of Modification
8
Peptide Start
32
Peptide End
44
CPTAC ID
CPTAC-49
Peptide Molecular Mass
1,537.6984
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 37.8 10.7 21.7 44.5 12.9 31.6 58.4 16.8 38.3 15 25 15
y9 (1+) 37 12.7 21.4 38.8 12.1 32.4 53.6 17.5 38.8 15 25 15
y7 (1+) 26.9 11.5 19.3 27.1 11.8 21.6 38.2 16.5 29 15 25 15
sum 28.7 10.2 18.4 31.4 10.6 24.8 42.5 14.7 30.9 15 25 15

Additional Resources and Comments


Assay Details for non-CPTAC-3780 Collapse assay details

Data source: Panorama

Official Gene Symbol
Coro1c
Peptide Sequence
HVFGQAVK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
12
Peptide End
19
CPTAC ID
non-CPTAC-3780
Peptide Molecular Mass
884.4868
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 11 7.6 10.6 20.2 8.9 13.1 23 11.7 16.9 15 15 15
y6 (1+) 4.8 6.9 3.3 8.4 9.5 9.4 9.7 11.7 10 15 15 15
b2 (1+) 6.3 5.9 4.3 9.4 8.3 4.2 11.3 10.2 6 15 15 15
b6 (1+) 9.3 4.9 4.6 11.1 8.7 5.4 14.5 10 7.1 15 15 15
b5 (1+) 38.3 14.1 30.4 47.9 80.2 59 61.3 81.4 66.4 15 15 15
sum 6.1 2.4 3.7 10.9 9.3 9 12.5 9.6 9.7 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-3699 Collapse assay details

Data source: Panorama

Official Gene Symbol
Coro1c
Peptide Sequence
YFEITDESPYVHYLNTFSSK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
292
Peptide End
311
CPTAC ID
non-CPTAC-3699
Peptide Molecular Mass
2,439.1271
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 13.3 4.1 5.6 20.7 17.1 19.7 24.6 17.6 20.5 15 15 15
b2 (1+) 5.3 3 2.4 17.5 19.1 16.6 18.3 19.3 16.8 15 15 15
y16 (2+) 7.8 3 2.5 17.6 20.4 17.3 19.3 20.6 17.5 15 15 15
y17 (2+) 10.9 2 2.9 18 20.1 16.8 21 20.2 17 15 15 15
y18 (2+) 7.5 3.4 3.8 20.2 21.1 17.5 21.5 21.4 17.9 15 15 15
sum 5.9 1.6 1.6 17.4 20 17.2 18.4 20.1 17.3 15 15 15

Additional Resources and Comments