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Overview Data source: UniProt

Official Gene Symbol Other Aliases
CCND1 BCL1, PRAD1
Sequence Length (AA) Molecular Weight (Da)
295 33729
Protein Name
G1/S-specific cyclin-D1
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MEHQLLCCEV ETIRRAYPDA NLLNDRVLRA MLKAEETCAP SVSYFKCVQK
60 70 80 90 100
EVLPSMRKIV ATWMLEVCEE QKCEEEVFPL AMNYLDRFLS LEPVKKSRLQ
110 120 130 140 150
LLGATCMFVA SKMKETIPLT AEKLCIYTDN SIRPEELLQM ELLLVNKLKW
160 170 180 190 200
NLAAMTPHDF IEHFLSKMPE AEENKQIIRK HAQTFVALCA TDVKFISNPP
210 220 230 240 250
SMVAAGSVVA AVQGLNLRSP NNFLSYYRLT RFLSRVIKCD PDCLRACQEQ
260 270 280 290 295
IEALLESSLR QAQQNMDPKA AEEEEEEEEE VDLACTPTDV RDVDI

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-5761 Collapse assay details

Data source: Panorama

Official Gene Symbol
CCND1
Peptide Modified Sequence
AC[+57.021464]QEQIEALLESSLR
Modification Type
Carbamidomethyl (C)
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
246
Peptide End
260
CPTAC ID
CPTAC-5761
Peptide Molecular Mass
1,745.8618
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
Cell Lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center, H. Lee Moffitt Cancer Center & Research Institute, Broad Institute
Submitting Lab PI
Amanda Paulovich, John Koomen, Steven A. Carr

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
SCIEX 5500 / Thermo Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Dionex Ultimate 3000
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 22.7 12.8 15.8 22.7 22.4 15.8 32.1 25.8 22.3 14 14 14
y6 (1+) 25.6 12.7 14.7 25.8 21.8 16.1 36.3 25.2 21.8 14 14 14
y5 (1+) 29.1 12.5 15.3 27.6 22.2 16.4 40.1 25.5 22.4 14 14 14
y4 (1+) 29.2 12.5 15.1 23.3 22.6 16.4 37.4 25.8 22.3 14 14 14
b8 (1+) 25.3 12.2 13.4 27.1 21.8 15.6 37.1 25 20.6 14 14 14
sum 26.9 12.5 14.9 25 22.1 16.1 36.7 25.4 21.9 14 14 14


Additional Resources and Comments


Assay Details for CPTAC-5764 Collapse assay details

Data source: Panorama

Official Gene Symbol
CCND1
Peptide Modified Sequence
AEETC[+57.021464]APSVSYFK
Modification Type
Carbamidomethyl (C)
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
34
Peptide End
46
CPTAC ID
CPTAC-5764
Peptide Molecular Mass
1,487.6602
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
Cell Lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center, H. Lee Moffitt Cancer Center & Research Institute, Broad Institute
Submitting Lab PI
Amanda Paulovich, John Koomen, Steven A. Carr

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
SCIEX 5500 / Thermo Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Dionex Ultimate 3000
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 21.9 7.3 6.9 25.8 27.1 15.5 33.8 28.1 17 15 15 15
y7 (1+) 16.5 8.5 6.2 27 26.8 15.7 31.6 28.1 16.9 15 15 15
y4 (1+) 17.4 10 6.3 27.4 27.5 15.5 32.5 29.3 16.7 15 15 15
sum 17.3 8.3 6.1 26.5 26.8 15.6 31.6 28.1 16.8 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-5765 Collapse assay details

Data source: Panorama

Official Gene Symbol
CCND1
Peptide Sequence
AYPDANLLNDR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
16
Peptide End
26
CPTAC ID
CPTAC-5765
Peptide Molecular Mass
1,260.6099
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
Cell Lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center, H. Lee Moffitt Cancer Center & Research Institute, Broad Institute
Submitting Lab PI
Amanda Paulovich, John Koomen, Steven A. Carr

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
SCIEX 5500 / Thermo Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Dionex Ultimate 3000
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 45.4 25 20.9 56.3 26.8 21.8 72.3 36.7 30.2 15 15 15
y7 (1+) 80 29.4 20.8 85.2 30.3 23.8 116.9 42.2 31.6 15 15 15
y9 (1+) 68.9 23.5 14.3 74.3 24.5 17.8 101.3 33.9 22.8 15 15 15
sum 44.9 20.5 14.1 49.8 24 18.4 67.1 31.6 23.2 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-5763 Collapse assay details

Data source: Panorama

Official Gene Symbol
CCND1
Peptide Sequence
FLSLEPVKK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
88
Peptide End
96
CPTAC ID
CPTAC-5763
Peptide Molecular Mass
1,059.6328
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
Cell Lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center, H. Lee Moffitt Cancer Center & Research Institute, Broad Institute
Submitting Lab PI
Amanda Paulovich, John Koomen, Steven A. Carr

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
SCIEX 5500 / Thermo Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Dionex Ultimate 3000
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 93.7 23.5 26.5 104.4 30.3 28.5 140.3 38.3 38.9 14 14 14
y5 (1+) 39.7 14.2 10.1 39 16.9 10.4 55.7 22.1 14.5 14 14 14
y4 (1+) 31.3 10.2 9.2 34 19.9 13.5 46.2 22.4 16.3 14 14 14
y3 (1+) 54.4 18.8 19.2 68.8 18.8 29.2 87.7 26.6 34.9 14 14 14
sum 29.1 8.8 6.1 30.6 18 9.1 42.2 20 11 14 14 14


Additional Resources and Comments


Assay Details for CPTAC-5762 Collapse assay details

Data source: Panorama

Official Gene Symbol
CCND1
Peptide Modified Sequence
VIKC[+57.021464]DPDC[+57.021464]LR
Modification Type
Carbamidomethyl (C)
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
236
Peptide End
245
CPTAC ID
CPTAC-5762
Peptide Molecular Mass
1,274.6111
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
Cell Lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center, H. Lee Moffitt Cancer Center & Research Institute, Broad Institute
Submitting Lab PI
Amanda Paulovich, John Koomen, Steven A. Carr

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
SCIEX 5500 / Thermo Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Dionex Ultimate 3000
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 42.6 14.9 8.2 49.6 20.7 18.9 65.4 25.5 20.6 15 15 15
y5 (1+) 18.9 11.5 7.8 28.7 17.2 14.8 34.4 20.7 16.7 15 15 15
y3 (1+) 22.4 11.9 7.3 35.1 18.6 16 41.6 22.1 17.6 15 15 15
sum 19.8 10.7 6.2 31.4 16.9 15.4 37.1 20 16.6 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-5363 Collapse assay details

Data source: Panorama

Official Gene Symbol
CCND1
Peptide Modified Sequence
AC[+57.021464]QEQIEALLESSLR
Modification Type
Carbamidomethyl (C)
Protein - Site of Modification
248
Peptide - Site of Modification
2
Peptide Start
246
Peptide End
260
CPTAC ID
non-CPTAC-5363
Peptide Molecular Mass
1,745.8618
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Cell Lysate
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
RSLCnano and Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
RSLCnano
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nl/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 10.8 6.8 10.1 15.2 10.9 10.2 18.6 12.8 14.4 15 15 15
b8 (1+) 13.3 10.8 12.1 15.3 13.4 13.4 20.3 17.2 18.1 15 15 15
y3 (1+) 14.9 4.7 12.1 17 10.1 12.3 22.6 11.1 17.3 15 15 15
y5 (1+) 9.4 5.5 9.4 11.9 9.6 9.9 15.2 11.1 13.7 15 15 15
sum 5.9 4.6 8.1 9.9 8.6 8.9 11.5 9.8 12 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-5536 Collapse assay details

Data source: Panorama

Official Gene Symbol
CCND1
Peptide Sequence
AYPDANLLNDR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
6
Peptide Start
16
Peptide End
26
CPTAC ID
non-CPTAC-5536
Peptide Molecular Mass
1,260.6099
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
Cell Line
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
RSLCnano and Quantiva
Internal Standard
SIS Peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
RSLCnano
Column Packing
C18 PepMap100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 34.6 12.3 16.3 47.5 17.8 17.8 58.8 21.6 24.1 14 14 14
y6 (1+) 109.5 19.8 12.9 93 27 17.3 143.7 33.5 21.6 14 14 14
y7 (1+) 73.6 18.6 14.3 78 24.3 13.7 107.2 30.6 19.8 14 14 14
y8 (1+) 57 23.3 14.2 48.2 23.3 21.3 74.6 33 25.6 14 14 14
y9 (1+) 52.4 19.1 7.4 67 22.1 11.4 85.1 29.2 13.6 14 14 14
sum 31.3 8.2 6.1 36.5 17 11.4 48.1 18.9 12.9 14 14 14


Additional Resources and Comments


Assay Details for non-CPTAC-5538 Collapse assay details

Data source: Panorama

Official Gene Symbol
CCND1
Peptide Sequence
FLSLEPVKK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
6
Peptide Start
88
Peptide End
96
CPTAC ID
non-CPTAC-5538
Peptide Molecular Mass
1,059.6328
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
Cell Line
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
RSLCnano and Quantiva
Internal Standard
SIS Peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
RSLCnano
Column Packing
C18 PepMap100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 54.4 18.8 19.2 68.8 18.8 29.2 87.7 26.6 34.9 14 14 14
y6 (1+) 93.7 23.5 26.5 104.4 30.3 28.5 140.3 38.3 38.9 14 14 14
y5 (1+) 39.7 14.2 10.1 39 16.9 10.4 55.7 22.1 14.5 14 14 14
y4 (1+) 31.3 10.2 9.2 34 19.9 13.5 46.2 22.4 16.3 14 14 14
sum 29.1 8.8 6.1 30.6 18 9.1 42.2 20 11 14 14 14


Additional Resources and Comments