CCND1, CPTAC-5363 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
CCND1	BCL1, PRAD1

Sequence Length (AA)	Molecular Weight (Da)
295	33729

Protein Name
G1/S-specific cyclin-D1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MEHQLLCCEV	ETIRRAYPDA	NLLNDRVLRA	MLKAEETCAP	SVSYFKCVQK
60	70	80	90	100
EVLPSMRKIV	ATWMLEVCEE	QKCEEEVFPL	AMNYLDRFLS	LEPVKKSRLQ
110	120	130	140	150
LLGATCMFVA	SKMKETIPLT	AEKLCIYTDN	SIRPEELLQM	ELLLVNKLKW
160	170	180	190	200
NLAAMTPHDF	IEHFLSKMPE	AEENKQIIRK	HAQTFVALCA	TDVKFISNPP
210	220	230	240	250
SMVAAGSVVA	AVQGLNLRSP	NNFLSYYRLT	RFLSRVIKCD	PDCLRACQEQ
260	270	280	290	295
IEALLESSLR	QAQQNMDPKA	AEEEEEEEEE	VDLACTPTDV	RDVDI

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt

Assay Details for CPTAC-5761 Collapse assay details

Data source: Panorama

Official Gene Symbol: CCND1
Peptide Modified Sequence: AC[+57.021464]QEQIEALLESSLR
Modification Type: Carbamidomethyl (C)
Protein - Site of Modification: N/A
Peptide - Site of Modification: 3
Peptide Start: 246
Peptide End: 260

CPTAC ID: CPTAC-5761
Peptide Molecular Mass: 1,745.8618
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM
Enrichment Method: N/A
Matrix: Cell Lysate
Submitting Laboratory: Fred Hutchinson Cancer Research Center, H. Lee Moffitt Cancer Center & Research Institute, Broad Institute
Submitting Lab PI: Amanda Paulovich, John Koomen, Steven A. Carr

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: SCIEX 5500 / Thermo Quantiva
Internal Standard: Stable Isotope Labeled Standard
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Dionex Ultimate 3000
Column Packing: C18 PepMap 100
Column Dimensions: 75 micron ID x 25 cm length
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y7 (1+)	22.7	12.8	15.8	22.7	22.4	15.8	32.1	25.8	22.3	14	14	14
y6 (1+)	25.6	12.7	14.7	25.8	21.8	16.1	36.3	25.2	21.8	14	14	14
y5 (1+)	29.1	12.5	15.3	27.6	22.2	16.4	40.1	25.5	22.4	14	14	14
y4 (1+)	29.2	12.5	15.1	23.3	22.6	16.4	37.4	25.8	22.3	14	14	14
b8 (1+)	25.3	12.2	13.4	27.1	21.8	15.6	37.1	25	20.6	14	14	14
sum	26.9	12.5	14.9	25	22.1	16.1	36.7	25.4	21.9	14	14	14

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y6 (1+)	93.7	23.5	26.5	104.4	30.3	28.5	140.3	38.3	38.9	14	14	14
y5 (1+)	39.7	14.2	10.1	39	16.9	10.4	55.7	22.1	14.5	14	14	14
y4 (1+)	31.3	10.2	9.2	34	19.9	13.5	46.2	22.4	16.3	14	14	14
y3 (1+)	54.4	18.8	19.2	68.8	18.8	29.2	87.7	26.6	34.9	14	14	14
sum	29.1	8.8	6.1	30.6	18	9.1	42.2	20	11	14	14	14

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y6 (1+)	10.8	6.8	10.1	15.2	10.9	10.2	18.6	12.8	14.4	15	15	15
b8 (1+)	13.3	10.8	12.1	15.3	13.4	13.4	20.3	17.2	18.1	15	15	15
y3 (1+)	14.9	4.7	12.1	17	10.1	12.3	22.6	11.1	17.3	15	15	15
y5 (1+)	9.4	5.5	9.4	11.9	9.6	9.9	15.2	11.1	13.7	15	15	15
sum	5.9	4.6	8.1	9.9	8.6	8.9	11.5	9.8	12	15	15	15

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y4 (1+)	34.6	12.3	16.3	47.5	17.8	17.8	58.8	21.6	24.1	14	14	14
y6 (1+)	109.5	19.8	12.9	93	27	17.3	143.7	33.5	21.6	14	14	14
y7 (1+)	73.6	18.6	14.3	78	24.3	13.7	107.2	30.6	19.8	14	14	14
y8 (1+)	57	23.3	14.2	48.2	23.3	21.3	74.6	33	25.6	14	14	14
y9 (1+)	52.4	19.1	7.4	67	22.1	11.4	85.1	29.2	13.6	14	14	14
sum	31.3	8.2	6.1	36.5	17	11.4	48.1	18.9	12.9	14	14	14

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y3 (1+)	54.4	18.8	19.2	68.8	18.8	29.2	87.7	26.6	34.9	14	14	14
y6 (1+)	93.7	23.5	26.5	104.4	30.3	28.5	140.3	38.3	38.9	14	14	14
y5 (1+)	39.7	14.2	10.1	39	16.9	10.4	55.7	22.1	14.5	14	14	14
y4 (1+)	31.3	10.2	9.2	34	19.9	13.5	46.2	22.4	16.3	14	14	14
sum	29.1	8.8	6.1	30.6	18	9.1	42.2	20	11	14	14	14

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CCND1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-5761 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

Assay Details for CPTAC-5764 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

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Repeatability

Additional Resources and Comments

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Assay Details for CPTAC-5765 Collapse assay details

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Assay Details for CPTAC-5763 Collapse assay details

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Assay Details for CPTAC-5762 Collapse assay details

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Assay Details for non-CPTAC-5363 Collapse assay details

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Assay Parameters Collapse assay parameters

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Repeatability

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Assay Details for non-CPTAC-5536 Collapse assay details

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Assay Parameters Collapse assay parameters

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Repeatability

Additional Resources and Comments

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Assay Details for non-CPTAC-5538 Collapse assay details

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Assay Parameters Collapse assay parameters

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Repeatability

Additional Resources and Comments

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Assay Details for CPTAC-2804 Collapse assay details

Assay Parameters Collapse assay parameters

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Repeatability

Additional Resources and Comments

Comments

Assay Details for CPTAC-2805 Collapse assay details

Assay Parameters Collapse assay parameters

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