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Overview Data source: UniProt

Official Gene Symbol Other Aliases
FABP4 N/A
Sequence Length (AA) Molecular Weight (Da)
132 14719
Protein Name
Fatty acid-binding protein, adipocyte
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MCDAFVGTWK LVSSENFDDY MKEVGVGFAT RKVAGMAKPN MIISVNGDVI
60 70 80 90 100
TIKSESTFKN TEISFILGQE FDEVTADDRK VKSTITLDGG VLVHVQKWDG
110 120 130 132
KSTTIKRKRE DDKLVVECVM KGVTSTRVYE RA

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-5845 Collapse assay details

Data source: Panorama

Official Gene Symbol
FABP4
Peptide Sequence
EVGVGFATR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
6
Peptide Start
23
Peptide End
31
CPTAC ID
CPTAC-5845
Peptide Molecular Mass
934.4872
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
Frozen breast cancer tumor tissue lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Quantification of Human Epidermal Growth Factor Receptor 2 by Immunopeptide Enrichment and Targeted Mass Spectrometry in Formalin-Fixed Paraffin-Embedded and Frozen Breast Cancer Tissues. Kennedy JJ, Whiteaker JR, Kennedy LC, Bosch DE, Lerch ML, Schoenherr RM, Zhao L, Lin C, Chowdhury S, Kilgore MR, Allison KH, Wang P, Hoofnagle AN, Baird GS, Paulovich AG. Clin Chem. 2021 Jun 17:hvab047. doi: 10.1093/clinchem/hvab047. Online ahead of print. PMID: 34136904


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTRAP 6500
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Eksigent nanoLC 425
Column Packing
3 µm ChromXP C18EP, 120 Å
Column Dimensions
150 x 0.3 mm
Flow Rate
10.0 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 10.9 9.5 5.2 12.5 23.5 14 16.6 25.3 14.9 15 17 15
y4 (1+) 10.8 10 6 15.8 23.7 13.3 19.1 25.7 14.6 15 17 15
y5 (1+) 10.8 9.3 5.4 13.6 19.4 16.2 17.4 21.5 17.1 15 17 15
y6 (1+) 13.4 9.6 6.2 13.2 18.4 17.4 18.8 20.8 18.5 15 17 15
y7 (1+) 10.8 10.6 6.7 15.3 22.7 17.3 18.7 25.1 18.6 15 17 15
sum 10.1 8.8 4.8 13.7 20.7 16 17 22.5 16.7 15 17 15


Additional Resources and Comments


Assay Details for CPTAC-5870 Collapse assay details

Data source: Panorama

Official Gene Symbol
FABP4
Peptide Sequence
EVGVGFATR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
6
Peptide Start
23
Peptide End
31
CPTAC ID
CPTAC-5870
Peptide Molecular Mass
934.4872
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
FFPE breast cancer tumor tissue lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Quantification of Human Epidermal Growth Factor Receptor 2 by Immunopeptide Enrichment and Targeted Mass Spectrometry in Formalin-Fixed Paraffin-Embedded and Frozen Breast Cancer Tissues. Kennedy JJ, Whiteaker JR, Kennedy LC, Bosch DE, Lerch ML, Schoenherr RM, Zhao L, Lin C, Chowdhury S, Kilgore MR, Allison KH, Wang P, Hoofnagle AN, Baird GS, Paulovich AG. Clin Chem. 2021 Jun 17:hvab047. doi: 10.1093/clinchem/hvab047. Online ahead of print. PMID: 34136904


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTRAP 6500
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Eksigent nanoLC 425
Column Packing
3 µm ChromXP C18EP, 120 Å
Column Dimensions
150 x 0.3 mm
Flow Rate
10.0 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 10.5 4.3 4.1 15.8 7.8 8.6 19 8.9 9.5 12 12 12
y7 (1+) 11.5 6.4 7.1 13.2 12.2 10.1 17.5 13.8 12.3 12 12 12
y6 (1+) 14.8 6.1 7.9 15.6 11.8 11.9 21.5 13.3 14.3 12 12 12
y4 (1+) 11.8 5.5 7.2 13.4 9 8.5 17.9 10.5 11.1 12 12 12
y3 (1+) 10.5 4.5 5.9 14.2 7.6 9 17.7 8.8 10.8 12 12 12
sum 10.1 4 4.4 14.1 8.9 8.9 17.3 9.8 9.9 12 12 12


Additional Resources and Comments