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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

PLIN4

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
PLIN4 KIAA1881
Sequence Length (AA) Molecular Weight (Da)
1357 134431
Protein Name
Perilipin-4
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MQTLGSFFGS LPGFSSARNL VANAHSSARA RPAADPTGAP AAEAAQPQAQ
60 70 80 90 100
VAAHPEQTAP WTEKELQPSE KQMVSGAKDL VCSKMSRAKD AVSSGVASVV
110 120 130 140 150
DVAKGVVQGG LDTTRSALTG TKEVVSSGVT GAMDMAKGAV QGGLDTSKAV
160 170 180 190 200
LTGTKDTVST GLTGAVNVAK GTVQAGVDTT KTVLTGTKDT VTTGVMGAVN
210 220 230 240 250
LAKGTVQTGV ETSKAVLTGT KDAVSTGLTG AVNVARGSIQ TGVDTSKTVL
260 270 280 290 300
TGTKDTVCSG VTGAMNVAKG TIQTGVDTSK TVLTGTKDTV CSGVTGAMNV
310 320 330 340 350
AKGTIQTGVD TSKTVLTGTK DTVCSGVTGA MNVAKGTIQT GVDTTKTVLT
360 370 380 390 400
GTKNTVCSGV TGAVNLAKEA IQGGLDTTKS MVMGTKDTMS TGLTGAANVA
410 420 430 440 450
KGAMQTGLNT TQNIATGTKD TVCSGVTGAM NLARGTIQTG VDTTKIVLTG
460 470 480 490 500
TKDTVCSGVT GAANVAKGAV QGGLDTTKSV LTGTKDAVST GLTGAVNVAK
510 520 530 540 550
GTVQTGVDTT KTVLTGTKDT VCSGVTSAVN VAKGAVQGGL DTTKSVVIGT
560 570 580 590 600
KDTMSTGLTG AANVAKGAVQ TGVDTAKTVL TGTKDTVTTG LVGAVNVAKG
610 620 630 640 650
TVQTGMDTTK TVLTGTKDTI YSGVTSAVNV AKGAVQTGLK TTQNIATGTK
660 670 680 690 700
NTFGSGVTSA VNVAKGAAQT GVDTAKTVLT GTKDTVTTGL MGAVNVAKGT
710 720 730 740 750
VQTSVDTTKT VLTGTKDTVC SGVTGAANVA KGAIQGGLDT TKSVLTGTKD
760 770 780 790 800
AVSTGLTGAV KLAKGTVQTG MDTTKTVLTG TKDAVCSGVT GAANVAKGAV
810 820 830 840 850
QMGVDTAKTV LTGTKDTVCS GVTGAANVAK GAVQTGLKTT QNIATGTKNT
860 870 880 890 900
LGSGVTGAAK VAKGAVQGGL DTTKSVLTGT KDAVSTGLTG AVNLAKGTVQ
910 920 930 940 950
TGVDTSKTVL TGTKDTVCSG VTGAVNVAKG TVQTGVDTAK TVLSGAKDAV
960 970 980 990 1000
TTGVTGAVNV AKGTVQTGVD ASKAVLMGTK DTVFSGVTGA MSMAKGAVQG
1010 1020 1030 1040 1050
GLDTTKTVLT GTKDAVSAGL MGSGNVATGA THTGLSTFQN WLPSTPATSW
1060 1070 1080 1090 1100
GGLTSSRTTD NGGEQTALSP QEAPFSGIST PPDVLSVGPE PAWEAAATTK
1110 1120 1130 1140 1150
GLATDVATFT QGAAPGREDT GLLATTHGPE EAPRLAMLQN ELEGLGDIFH
1160 1170 1180 1190 1200
PMNAEEQAQL AASQPGPKVL SAEQGSYFVR LGDLGPSFRQ RAFEHAVSHL
1210 1220 1230 1240 1250
QHGQFQARDT LAQLQDCFRL IEKAQQAPEG QPRLDQGSGA SAEDAAVQEE
1260 1270 1280 1290 1300
RDAGVLSRVC GLLRQLHTAY SGLVSSLQGL PAELQQPVGR ARHSLCELYG
1310 1320 1330 1340 1350
IVASAGSVEE LPAERLVQSR EGVHQAWQGL EQLLEGLQHN PPLSWLVGPF
1357
ALPAGGQ

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-5849 Collapse assay details

Data source: Panorama

Official Gene Symbol
PLIN4
Peptide Sequence
VLSAEQGSYFVR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
6
Peptide Start
1169
Peptide End
1180
CPTAC ID
CPTAC-5849
Peptide Molecular Mass
1,354.6881
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
Frozen breast cancer tumor tissue lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Quantification of Human Epidermal Growth Factor Receptor 2 by Immunopeptide Enrichment and Targeted Mass Spectrometry in Formalin-Fixed Paraffin-Embedded and Frozen Breast Cancer Tissues. Kennedy JJ, Whiteaker JR, Kennedy LC, Bosch DE, Lerch ML, Schoenherr RM, Zhao L, Lin C, Chowdhury S, Kilgore MR, Allison KH, Wang P, Hoofnagle AN, Baird GS, Paulovich AG. Clin Chem. 2021 Jun 17:hvab047. doi: 10.1093/clinchem/hvab047. Online ahead of print. PMID: 34136904

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTRAP 6500
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Eksigent nanoLC 425
Column Packing
3 µm ChromXP C18EP, 120 Å
Column Dimensions
150 x 0.3 mm
Flow Rate
10.0 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 30.4 8.9 7.6 33.2 11.1 7.8 45 14.2 10.9 15 17 15
y9 (1+) 80.6 25.5 9.6 81.5 26.9 7.4 114.6 37.1 12.1 15 17 15
y8 (1+) 50.4 17.6 17.9 44.9 21.5 17.3 67.5 27.8 24.9 15 17 15
y7 (1+) 48.5 15 10.9 47.4 24.8 12.3 67.8 29 16.4 15 17 15
y6 (1+) 40.4 9.3 10.9 43.8 15 9.1 59.6 17.6 14.2 15 17 15
sum 21.4 6.3 5.9 26.6 10.7 5.7 34.1 12.4 8.2 15 17 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for CPTAC-5874 Collapse assay details

Data source: Panorama

Official Gene Symbol
PLIN4
Peptide Sequence
VLSAEQGSYFVR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
6
Peptide Start
1169
Peptide End
1180
CPTAC ID
CPTAC-5874
Peptide Molecular Mass
1,354.6881
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
FFPE breast cancer tumor tissue lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Quantification of Human Epidermal Growth Factor Receptor 2 by Immunopeptide Enrichment and Targeted Mass Spectrometry in Formalin-Fixed Paraffin-Embedded and Frozen Breast Cancer Tissues. Kennedy JJ, Whiteaker JR, Kennedy LC, Bosch DE, Lerch ML, Schoenherr RM, Zhao L, Lin C, Chowdhury S, Kilgore MR, Allison KH, Wang P, Hoofnagle AN, Baird GS, Paulovich AG. Clin Chem. 2021 Jun 17:hvab047. doi: 10.1093/clinchem/hvab047. Online ahead of print. PMID: 34136904

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTRAP 6500
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Eksigent nanoLC 425
Column Packing
3 µm ChromXP C18EP, 120 Å
Column Dimensions
150 x 0.3 mm
Flow Rate
10.0 µL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 30.3 14.1 7.1 24.4 11.6 9.3 38.9 18.3 11.7 12 12 12
y7 (1+) 24 15.4 10.4 34.2 14 10.6 41.8 20.8 14.8 12 12 12
y8 (1+) 30.6 11.7 8.4 40.4 14 8.2 50.7 18.2 11.7 12 12 12
y9 (1+) 37 13.7 16.7 21.9 25.9 17 43 29.3 23.8 12 12 12
y10 (1+) 25.5 7.9 5.2 31 8.5 7.5 40.1 11.6 9.1 12 12 12
sum 19.7 7.7 3.9 17.5 6.7 3.8 26.4 10.2 5.4 12 12 12

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments