ACTA1, CPTAC-5857 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
ACTA1	ACTA

Sequence Length (AA)	Molecular Weight (Da)
377	42051

Protein Name
Actin, alpha skeletal muscle

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MCDEDETTAL	VCDNGSGLVK	AGFAGDDAPR	AVFPSIVGRP	RHQGVMVGMG
60	70	80	90	100
QKDSYVGDEA	QSKRGILTLK	YPIEHGIITN	WDDMEKIWHH	TFYNELRVAP
110	120	130	140	150
EEHPTLLTEA	PLNPKANREK	MTQIMFETFN	VPAMYVAIQA	VLSLYASGRT
160	170	180	190	200
TGIVLDSGDG	VTHNVPIYEG	YALPHAIMRL	DLAGRDLTDY	LMKILTERGY
210	220	230	240	250
SFVTTAEREI	VRDIKEKLCY	VALDFENEMA	TAASSSSLEK	SYELPDGQVI
260	270	280	290	300
TIGNERFRCP	ETLFQPSFIG	MESAGIHETT	YNSIMKCDID	IRKDLYANNV
310	320	330	340	350
MSGGTTMYPG	IADRMQKEIT	ALAPSTMKIK	IIAPPERKYS	VWIGGSILAS
360	370	377
LSTFQQMWIT	KQEYDEAGPS	IVHRKCF

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-5857 Collapse assay details

Data source: Panorama

Official Gene Symbol: ACTA1
Peptide Sequence: AVFPSIVGRPR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 31
Peptide End: 41

CPTAC ID: CPTAC-5857
Peptide Molecular Mass: 1,197.6982
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM
Enrichment Method: peptide immunoaffinity
Matrix: Frozen breast cancer tumor tissue lysate pool
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Quantification of Human Epidermal Growth Factor Receptor 2 by Immunopeptide Enrichment and Targeted Mass Spectrometry in Formalin-Fixed Paraffin-Embedded and Frozen Breast Cancer Tissues. Kennedy JJ, Whiteaker JR, Kennedy LC, Bosch DE, Lerch ML, Schoenherr RM, Zhao L, Lin C, Chowdhury S, Kilgore MR, Allison KH, Wang P, Hoofnagle AN, Baird GS, Paulovich AG. Clin Chem. 2021 Jun 17:hvab047. doi: 10.1093/clinchem/hvab047. Online ahead of print. PMID: 34136904

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: QTRAP 6500
Internal Standard: peptide
Peptide Standard Purity: Crude
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Eksigent nanoLC 425
Column Packing: 3 µm ChromXP C18EP, 120 Å
Column Dimensions: 150 x 0.3 mm
Flow Rate: 10.0 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y7 (2+)	40.3	31.7	8.2	43.9	35.4	8.9	59.6	47.5	12.1	15	17	15
y8 (2+)	30.8	8.5	6.5	23.6	10.4	7.8	38.8	13.4	10.2	15	17	15
y9 (3+)	38.8	12.5	4.8	39.4	19.1	5.9	55.3	22.8	7.6	15	17	15
b3 (1+)	56.6	10.7	6.4	64.3	11.5	9.5	85.7	15.7	11.5	15	17	15
sum	16.1	6.7	4.4	23.2	11.4	6.5	28.2	13.2	7.8	15	17	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

View in CPTC Antibody Portal

Comments