ENTPD1, CPTAC-5966 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
ENTPD1	CD39

Sequence Length (AA)	Molecular Weight (Da)
510	57965

Protein Name
Ectonucleoside triphosphate diphosphohydrolase 1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MEDTKESNVK	TFCSKNILAI	LGFSSIIAVI	ALLAVGLTQN	KALPENVKYG
60	70	80	90	100
IVLDAGSSHT	SLYIYKWPAE	KENDTGVVHQ	VEECRVKGPG	ISKFVQKVNE
110	120	130	140	150
IGIYLTDCME	RAREVIPRSQ	HQETPVYLGA	TAGMRLLRME	SEELADRVLD
160	170	180	190	200
VVERSLSNYP	FDFQGARIIT	GQEEGAYGWI	TINYLLGKFS	QKTRWFSIVP
210	220	230	240	250
YETNNQETFG	ALDLGGASTQ	VTFVPQNQTI	ESPDNALQFR	LYGKDYNVYT
260	270	280	290	300
HSFLCYGKDQ	ALWQKLAKDI	QVASNEILRD	PCFHPGYKKV	VNVSDLYKTP
310	320	330	340	350
CTKRFEMTLP	FQQFEIQGIG	NYQQCHQSIL	ELFNTSYCPY	SQCAFNGIFL
360	370	380	390	400
PPLQGDFGAF	SAFYFVMKFL	NLTSEKVSQE	KVTEMMKKFC	AQPWEEIKTS
410	420	430	440	450
YAGVKEKYLS	EYCFSGTYIL	SLLLQGYHFT	ADSWEHIHFI	GKIQGSDAGW
460	470	480	490	500
TLGYMLNLTN	MIPAEQPLST	PLSHSTYVFL	MVLFSLVLFT	VAIIGLLIFH
510
KPSYFWKDMV

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-5966 Collapse assay details

Data source: Panorama

Official Gene Symbol: ENTPD1
Peptide Sequence: SLSNYPFDFQGAR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: 4
Peptide Start: 155
Peptide End: 167

CPTAC ID: CPTAC-5966
Peptide Molecular Mass: 1,500.6997
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM
Enrichment Method: peptide immunoaffinity
Matrix: FFPE tumor tissue lysate pool
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Targeted Mass Spectrometry Enables Multiplexed Quantification of Immunomodulatory Proteins in Clinical Biospecimens.Whiteaker JR, Lundeen RA, Zhao L, Schoenherr RM, Burian A, Huang D, Voytovich U, Wang T, Kennedy JJ, Ivey RG, Lin C, Murillo OD, Lorentzen TD, Thiagarajan M, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Reading JJ, Kaczmarczyk JA, Richardson CW, Garcia-Buntley SS, Bocik W, Hewitt SM, Murray KE, Do N, Brophy M, Wilz SW, Yu H, Ajjarapu S, Boja E, Hiltke T, Rodriguez H, Paulovich AG.Front Immunol. 2021 Nov 11;12:765898. doi: 10.3389/fimmu.2021.765898. eCollection 2021.PMID: 34858420

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: QTRAP 5500
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Eksigent nanoLC 425
Column Packing: 3 µm ChromXP C18EP, 120 Å
Column Dimensions: 150 x 0.3 mm
Flow Rate: 0.3 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y7 (1+)	11.9	11.7	4	15	13.1	10.8	19.1	17.6	11.5	13	25	14
y8 (1+)	7.7	6.9	3.3	9.9	10.4	10.4	12.5	12.5	10.9	13	25	14
y9 (1+)	9	9.2	4.5	14.3	11	11.5	16.9	14.3	12.3	13	25	14
b5 (1+)	9.3	6.5	3.5	17.9	9.6	11.7	20.2	11.6	12.2	13	25	14
y5 (1+)	6.1	9.4	7.5	9.2	12.6	13.4	11	15.7	15.4	13	25	14
y6 (1+)	9.5	8.9	4.1	16	13.2	9.7	18.6	15.9	10.5	13	25	14
sum	6.1	6.1	2.8	11.5	9.7	10.4	13	11.5	10.8	13	25	14

ENTPD1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-5966 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

Search form

ENTPD1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-5966 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments