CX3CL1, CPTAC-5967 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
CX3CL1	FKN, NTT, SCYD1

Sequence Length (AA)	Molecular Weight (Da)
397	42203

Protein Name
Fractalkine

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAPISLSWLL	RLATFCHLTV	LLAGQHHGVT	KCNITCSKMT	SKIPVALLIH
60	70	80	90	100
YQQNQASCGK	RAIILETRQH	RLFCADPKEQ	WVKDAMQHLD	RQAAALTRNG
110	120	130	140	150
GTFEKQIGEV	KPRTTPAAGG	MDESVVLEPE	ATGESSSLEP	TPSSQEAQRA
160	170	180	190	200
LGTSPELPTG	VTGSSGTRLP	PTPKAQDGGP	VGTELFRVPP	VSTAATWQSS
210	220	230	240	250
APHQPGPSLW	AEAKTSEAPS	TQDPSTQAST	ASSPAPEENA	PSEGQRVWGQ
260	270	280	290	300
GQSPRPENSL	EREEMGPVPA	HTDAFQDWGP	GSMAHVSVVP	VSSEGTPSRE
310	320	330	340	350
PVASGSWTPK	AEEPIHATMD	PQRLGVLITP	VPDAQAATRR	QAVGLLAFLG
360	370	380	390	397
LLFCLGVAMF	TYQSLQGCPR	KMAGEMAEGL	RYIPRSCGSN	SYVLVPV

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-5967 Collapse assay details

Data source: Panorama

Official Gene Symbol: CX3CL1
Peptide Sequence: ALGTSPELPTGVTGSSGTR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: 4
Peptide Start: 150
Peptide End: 168

CPTAC ID: CPTAC-5967
Peptide Molecular Mass: 1,786.9061
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM
Enrichment Method: peptide immunoaffinity
Matrix: FFPE tumor tissue lysate pool
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Targeted Mass Spectrometry Enables Multiplexed Quantification of Immunomodulatory Proteins in Clinical Biospecimens.Whiteaker JR, Lundeen RA, Zhao L, Schoenherr RM, Burian A, Huang D, Voytovich U, Wang T, Kennedy JJ, Ivey RG, Lin C, Murillo OD, Lorentzen TD, Thiagarajan M, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Reading JJ, Kaczmarczyk JA, Richardson CW, Garcia-Buntley SS, Bocik W, Hewitt SM, Murray KE, Do N, Brophy M, Wilz SW, Yu H, Ajjarapu S, Boja E, Hiltke T, Rodriguez H, Paulovich AG.Front Immunol. 2021 Nov 11;12:765898. doi: 10.3389/fimmu.2021.765898. eCollection 2021.PMID: 34858420

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: QTRAP 5500
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Eksigent nanoLC 425
Column Packing: 3 µm ChromXP C18EP, 120 Å
Column Dimensions: 150 x 0.3 mm
Flow Rate: 0.3 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b7 (1+)	9.6	12.3	10.3	11.3	15.2	16.8	14.8	19.6	19.7	13	25	14
y7 (1+)	6.8	9	4	10	12	14.3	12.1	15	14.8	13	25	14
y9 (1+)	27.1	14.8	8.3	27.2	19.3	14.7	38.4	24.3	16.9	13	25	14
y11 (1+)	4.1	5.6	3.1	6.2	11.2	10.6	7.4	12.5	11	13	25	14
y12 (1+)	14.6	7.2	6.7	17.4	13.2	14	22.7	15	15.5	13	25	14
b8 (1+)	5	7.5	4.8	9.8	11.3	12.1	11	13.6	13	13	25	14
y6 (1+)	17.6	13.1	13.9	17.7	17.4	19.1	25	21.8	23.6	13	25	14
sum	3.7	5.2	2.7	6.8	11.1	11.3	7.7	12.3	11.6	13	25	14

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

View in CPTC Antibody Portal

Comments