STAT6, CPTAC-5965 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
STAT6	N/A

Sequence Length (AA)	Molecular Weight (Da)
847	94135

Protein Name
Signal transducer and activator of transcription 6

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MSLWGLVSKM	PPEKVQRLYV	DFPQHLRHLL	GDWLESQPWE	FLVGSDAFCC
60	70	80	90	100
NLASALLSDT	VQHLQASVGE	QGEGSTILQH	ISTLESIYQR	DPLKLVATFR
110	120	130	140	150
QILQGEKKAV	MEQFRHLPMP	FHWKQEELKF	KTGLRRLQHR	VGEIHLLREA
160	170	180	190	200
LQKGAEAGQV	SLHSLIETPA	NGTGPSEALA	MLLQETTGEL	EAAKALVLKR
210	220	230	240	250
IQIWKRQQQL	AGNGAPFEES	LAPLQERCES	LVDIYSQLQQ	EVGAAGGELE
260	270	280	290	300
PKTRASLTGR	LDEVLRTLVT	SCFLVEKQPP	QVLKTQTKFQ	AGVRFLLGLR
310	320	330	340	350
FLGAPAKPPL	VRADMVTEKQ	ARELSVPQGP	GAGAESTGEI	INNTVPLENS
360	370	380	390	400
IPGNCCSALF	KNLLLKKIKR	CERKGTESVT	EEKCAVLFSA	SFTLGPGKLP
410	420	430	440	450
IQLQALSLPL	VVIVHGNQDN	NAKATILWDN	AFSEMDRVPF	VVAERVPWEK
460	470	480	490	500
MCETLNLKFM	AEVGTNRGLL	PEHFLFLAQK	IFNDNSLSME	AFQHRSVSWS
510	520	530	540	550
QFNKEILLGR	GFTFWQWFDG	VLDLTKRCLR	SYWSDRLIIG	FISKQYVTSL
560	570	580	590	600
LLNEPDGTFL	LRFSDSEIGG	ITIAHVIRGQ	DGSPQIENIQ	PFSAKDLSIR
610	620	630	640	650
SLGDRIRDLA	QLKNLYPKKP	KDEAFRSHYK	PEQMGKDGRG	YVPATIKMTV
660	670	680	690	700
ERDQPLPTPE	LQMPTMVPSY	DLGMAPDSSM	SMQLGPDMVP	QVYPPHSHSI
710	720	730	740	750
PPYQGLSPEE	SVNVLSAFQE	PHLQMPPSLG	QMSLPFDQPH	PQGLLPCQPQ
760	770	780	790	800
EHAVSSPDPL	LCSDVTMVED	SCLSQPVTAF	PQGTWIGEDI	FPPLLPPTEQ
810	820	830	840	847
DLTKLLLEGQ	GESGGGSLGA	QPLLQPSHYG	QSGISMSHMD	LRANPSW

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt

Assay Details for CPTAC-5965 Collapse assay details

Data source: Panorama

Official Gene Symbol: STAT6
Peptide Sequence: GYVPATIK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: 4
Peptide Start: 640
Peptide End: 647

CPTAC ID: CPTAC-5965
Peptide Molecular Mass: 847.4804
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM
Enrichment Method: peptide immunoaffinity
Matrix: FFPE tumor tissue lysate pool
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Targeted Mass Spectrometry Enables Multiplexed Quantification of Immunomodulatory Proteins in Clinical Biospecimens.Whiteaker JR, Lundeen RA, Zhao L, Schoenherr RM, Burian A, Huang D, Voytovich U, Wang T, Kennedy JJ, Ivey RG, Lin C, Murillo OD, Lorentzen TD, Thiagarajan M, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Reading JJ, Kaczmarczyk JA, Richardson CW, Garcia-Buntley SS, Bocik W, Hewitt SM, Murray KE, Do N, Brophy M, Wilz SW, Yu H, Ajjarapu S, Boja E, Hiltke T, Rodriguez H, Paulovich AG.Front Immunol. 2021 Nov 11;12:765898. doi: 10.3389/fimmu.2021.765898. eCollection 2021.PMID: 34858420

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: QTRAP 5500
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Eksigent nanoLC 425
Column Packing: 3 µm ChromXP C18EP, 120 Å
Column Dimensions: 150 x 0.3 mm
Flow Rate: 0.3 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y3 (1+)	32.6	14.3	12.6	33.2	23	26.8	46.5	27.1	29.6	13	25	14
y6 (1+)	16.5	4.4	2.8	40.9	36.7	31.8	44.1	37	31.9	13	25	14
y5 (1+)	10.8	3.7	2.6	23.2	19.9	17.3	25.6	20.2	17.5	13	25	14
y6 (2+)	11.4	5	3.1	21.8	10.6	14.2	24.6	11.7	14.5	13	25	14
y5 (2+)	11.5	12.1	3.6	13.5	13.1	13.3	17.7	17.8	13.8	13	25	14
b2 (1+)	6.9	4	2	7.9	9.7	14.4	10.5	10.5	14.5	13	25	14
b3 (1+)	8.4	3.3	1.7	8.3	10.6	14	11.8	11.1	14.1	13	25	14
sum	9.8	3.5	1.9	18	17	17.6	20.5	17.4	17.7	13	25	14

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

View in CPTC Antibody Portal

Comments

Assay Details for CPTAC-1639 Collapse assay details

Data source: Panorama

Official Gene Symbol: STAT6
Peptide Sequence: SVSWSQFNK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 496
Peptide End: 504

CPTAC ID: CPTAC-1639
Peptide Molecular Mass: 1,081.5193
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: Ovarian cancer tumor tissue lysate
Submitting Laboratory: Pacific Northwest National Laboratory
Submitting Lab PI: Tao Liu