STAT1, CPTAC-5964 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
STAT1	N/A

Sequence Length (AA)	Molecular Weight (Da)
750	87335

Protein Name
Signal transducer and activator of transcription 1-alpha/beta

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MSQWYELQQL	DSKFLEQVHQ	LYDDSFPMEI	RQYLAQWLEK	QDWEHAANDV
60	70	80	90	100
SFATIRFHDL	LSQLDDQYSR	FSLENNFLLQ	HNIRKSKRNL	QDNFQEDPIQ
110	120	130	140	150
MSMIIYSCLK	EERKILENAQ	RFNQAQSGNI	QSTVMLDKQK	ELDSKVRNVK
160	170	180	190	200
DKVMCIEHEI	KSLEDLQDEY	DFKCKTLQNR	EHETNGVAKS	DQKQEQLLLK
210	220	230	240	250
KMYLMLDNKR	KEVVHKIIEL	LNVTELTQNA	LINDELVEWK	RRQQSACIGG
260	270	280	290	300
PPNACLDQLQ	NWFTIVAESL	QQVRQQLKKL	EELEQKYTYE	HDPITKNKQV
310	320	330	340	350
LWDRTFSLFQ	QLIQSSFVVE	RQPCMPTHPQ	RPLVLKTGVQ	FTVKLRLLVK
360	370	380	390	400
LQELNYNLKV	KVLFDKDVNE	RNTVKGFRKF	NILGTHTKVM	NMEESTNGSL
410	420	430	440	450
AAEFRHLQLK	EQKNAGTRTN	EGPLIVTEEL	HSLSFETQLC	QPGLVIDLET
460	470	480	490	500
TSLPVVVISN	VSQLPSGWAS	ILWYNMLVAE	PRNLSFFLTP	PCARWAQLSE
510	520	530	540	550
VLSWQFSSVT	KRGLNVDQLN	MLGEKLLGPN	ASPDGLIPWT	RFCKENINDK
560	570	580	590	600
NFPFWLWIES	ILELIKKHLL	PLWNDGCIMG	FISKERERAL	LKDQQPGTFL
610	620	630	640	650
LRFSESSREG	AITFTWVERS	QNGGEPDFHA	VEPYTKKELS	AVTFPDIIRN
660	670	680	690	700
YKVMAAENIP	ENPLKYLYPN	IDKDHAFGKY	YSRPKEAPEP	MELDGPKGTG
710	720	730	740	750
YIKTELISVS	EVHPSRLQTT	DNLLPMSPEE	FDEVSRIVGS	VEFDSMMNTV

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt

Assay Details for CPTAC-5964 Collapse assay details

Data source: Panorama

Official Gene Symbol: STAT1
Peptide Sequence: YTYEHDPITK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: 4
Peptide Start: 287
Peptide End: 296

CPTAC ID: CPTAC-5964
Peptide Molecular Mass: 1,265.5928
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM
Enrichment Method: peptide immunoaffinity
Matrix: FFPE tumor tissue lysate pool
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Targeted Mass Spectrometry Enables Multiplexed Quantification of Immunomodulatory Proteins in Clinical Biospecimens.Whiteaker JR, Lundeen RA, Zhao L, Schoenherr RM, Burian A, Huang D, Voytovich U, Wang T, Kennedy JJ, Ivey RG, Lin C, Murillo OD, Lorentzen TD, Thiagarajan M, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Reading JJ, Kaczmarczyk JA, Richardson CW, Garcia-Buntley SS, Bocik W, Hewitt SM, Murray KE, Do N, Brophy M, Wilz SW, Yu H, Ajjarapu S, Boja E, Hiltke T, Rodriguez H, Paulovich AG.Front Immunol. 2021 Nov 11;12:765898. doi: 10.3389/fimmu.2021.765898. eCollection 2021.PMID: 34858420

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: QTRAP 5500
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Eksigent nanoLC 425
Column Packing: 3 µm ChromXP C18EP, 120 Å
Column Dimensions: 150 x 0.3 mm
Flow Rate: 0.3 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y5 (1+)	8.1	9.1	7.5	15.1	9.1	7.5	17.1	12.9	10.6	13	25	14
y4 (1+)	12.5	7.1	5.9	13.8	6.9	9.9	18.6	9.9	11.5	13	25	14
y8 (2+)	9.4	4.9	6.1	9.4	6.6	9.6	13.3	8.2	11.4	13	25	14
y6 (2+)	19.1	13.6	4.3	19.2	14.6	9.6	27.1	20	10.5	13	25	14
b4 (1+)	25.1	12.6	7.1	43.8	18.8	21.1	50.5	22.6	22.3	13	25	14
y6 (1+)	13	10.4	8.2	13.2	10.3	12.8	18.5	14.6	15.2	13	25	14
sum	8.6	4.4	4	10.4	6.1	8.8	13.5	7.5	9.7	13	25	14

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

View in CPTC Antibody Portal

Comments

Assay Details for CPTAC-1272 Collapse assay details

Data source: Panorama

Official Gene Symbol: STAT1
Peptide Modified Sequence: NLSFFLTPPC[+57.0]AR
Modification Type: Carbamidomethyl (C)
Protein - Site of Modification: 492
Peptide - Site of Modification: 10
Peptide Start: 483
Peptide End: 494

CPTAC ID: CPTAC-1272
Peptide Molecular Mass: 1,421.7126
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: Ovarian cancer tumor tissue lysate
Submitting Laboratory: Pacific Northwest National Laboratory
Submitting Lab PI: Tao Liu