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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

PVR

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
PVR PVS
Sequence Length (AA) Molecular Weight (Da)
417 45303
Protein Name
Poliovirus receptor
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MARAMAAAWP LLLVALLVLS WPPPGTGDVV VQAPTQVPGF LGDSVTLPCY
60 70 80 90 100
LQVPNMEVTH VSQLTWARHG ESGSMAVFHQ TQGPSYSESK RLEFVAARLG
110 120 130 140 150
AELRNASLRM FGLRVEDEGN YTCLFVTFPQ GSRSVDIWLR VLAKPQNTAE
160 170 180 190 200
VQKVQLTGEP VPMARCVSTG GRPPAQITWH SDLGGMPNTS QVPGFLSGTV
210 220 230 240 250
TVTSLWILVP SSQVDGKNVT CKVEHESFEK PQLLTVNLTV YYPPEVSISG
260 270 280 290 300
YDNNWYLGQN EATLTCDARS NPEPTGYNWS TTMGPLPPFA VAQGAQLLIR
310 320 330 340 350
PVDKPINTTL ICNVTNALGA RQAELTVQVK EGPPSEHSGI SRNAIIFLVL
360 370 380 390 400
GILVFLILLG IGIYFYWSKC SREVLWHCHL CPSSTEHASA SANGHVSYSA
410 417
VSRENSSSQD PQTEGTR

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-6034 Collapse assay details

Data source: Panorama

Official Gene Symbol
PVR
Peptide Modified Sequence
HGESGSM[+15.994915]AVFHQTQGPSYSESK
Modification Type
Oxidation (M)
Protein - Site of Modification
75
Peptide - Site of Modification
7
Peptide Start
68
Peptide End
90
CPTAC ID
CPTAC-6034
Peptide Molecular Mass
2,366.0233
Species
Human
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
FFPE lung tumor tissue lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Whiteaker JR, Zhao L, Schoenherr RM, Huang D, Lundeen RA, Voytovich U, Kennedy JJ, Ivey RG, Lin C, Murillo OD, Lorentzen TD, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Reading JJ, Perry CD, Richardson CW, Garcia-Buntley SS, Bocik W, Hewitt SM, Chowdhury S, Vandermeer J, Smith SD, Gopal AK, Ramchurren N, Fling SP, Wang P, Paulovich AG. A multiplexed assay for quantifying immunomodulatory proteins supports correlative studies in immunotherapy clinical trials. Front Oncol. 2023 May 2;13:1168710. doi: 10.3389/fonc.2023.1168710. PMID: 37205196; PMCID: PMC10185886.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTRAP 5500
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
LC
Eksigent nanoLC 425
Column Packing
3 µm ChromXP C18EP, 120 Å
Column Dimensions
150 x 0.075 mm
Flow Rate
0.3 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b14 (2+) 27.1 18.4 10.8 31.1 19.5 14.6 41.3 26.8 18.2 15 14 15
b15 (2+) 31.3 17.2 12.1 32.5 24.1 21 45.1 29.6 24.2 15 14 15
y13 (2+) 35.2 19.5 10 32.5 22.4 17.9 47.9 29.7 20.5 15 14 15
sum 25.8 17.2 7.2 28.4 19.2 16.7 38.4 25.8 18.2 15 14 15

Additional Resources and Comments

Resources
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View Peptide in Panorama
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Assay Details for CPTAC-6038 Collapse assay details

Data source: Panorama

Official Gene Symbol
PVR
Peptide Sequence
HGESGSMAVFHQTQGPSYSESK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
68
Peptide End
90
CPTAC ID
CPTAC-6038
Peptide Molecular Mass
2,350.0284
Species
Human
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
FFPE lung tumor tissue lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Whiteaker JR, Zhao L, Schoenherr RM, Huang D, Lundeen RA, Voytovich U, Kennedy JJ, Ivey RG, Lin C, Murillo OD, Lorentzen TD, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Reading JJ, Perry CD, Richardson CW, Garcia-Buntley SS, Bocik W, Hewitt SM, Chowdhury S, Vandermeer J, Smith SD, Gopal AK, Ramchurren N, Fling SP, Wang P, Paulovich AG. A multiplexed assay for quantifying immunomodulatory proteins supports correlative studies in immunotherapy clinical trials. Front Oncol. 2023 May 2;13:1168710. doi: 10.3389/fonc.2023.1168710. PMID: 37205196; PMCID: PMC10185886.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTRAP 5500
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
LC
Eksigent nanoLC 425
Column Packing
3 µm ChromXP C18EP, 120 Å
Column Dimensions
150 x 0.075 mm
Flow Rate
0.3 µL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b6 (1+) 15.7 5.3 7 18.7 5.1 7.7 24.4 7.4 10.4 15 15 15
b15 (2+) 15.4 7.2 8.8 18.1 7.8 8.7 23.8 10.6 12.4 15 15 15
y15 (2+) 26 6.1 7.4 26.4 6.1 8.7 37.1 8.6 11.4 15 15 15
y8 (2+) 16.3 9.8 11.9 15.3 9.6 10.8 22.4 13.7 16.1 15 15 15
sum 13.4 5.7 7.9 14.7 5.4 8 19.9 7.9 11.2 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments