ZAP70, CPTAC-6054 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
ZAP70	SRK

Sequence Length (AA)	Molecular Weight (Da)
619	69872

Protein Name
Tyrosine-protein kinase ZAP-70

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MPDPAAHLPF	FYGSISRAEA	EEHLKLAGMA	DGLFLLRQCL	RSLGGYVLSL
60	70	80	90	100
VHDVRFHHFP	IERQLNGTYA	IAGGKAHCGP	AELCEFYSRD	PDGLPCNLRK
110	120	130	140	150
PCNRPSGLEP	QPGVFDCLRD	AMVRDYVRQT	WKLEGEALEQ	AIISQAPQVE
160	170	180	190	200
KLIATTAHER	MPWYHSSLTR	EEAERKLYSG	AQTDGKFLLR	PRKEQGTYAL
210	220	230	240	250
SLIYGKTVYH	YLISQDKAGK	YCIPEGTKFD	TLWQLVEYLK	LKADGLIYCL
260	270	280	290	300
KEACPNSSAS	NASGAAAPTL	PAHPSTLTHP	QRRIDTLNSD	GYTPEPARIT
310	320	330	340	350
SPDKPRPMPM	DTSVYESPYS	DPEELKDKKL	FLKRDNLLIA	DIELGCGNFG
360	370	380	390	400
SVRQGVYRMR	KKQIDVAIKV	LKQGTEKADT	EEMMREAQIM	HQLDNPYIVR
410	420	430	440	450
LIGVCQAEAL	MLVMEMAGGG	PLHKFLVGKR	EEIPVSNVAE	LLHQVSMGMK
460	470	480	490	500
YLEEKNFVHR	DLAARNVLLV	NRHYAKISDF	GLSKALGADD	SYYTARSAGK
510	520	530	540	550
WPLKWYAPEC	INFRKFSSRS	DVWSYGVTMW	EALSYGQKPY	KKMKGPEVMA
560	570	580	590	600
FIEQGKRMEC	PPECPPELYA	LMSDCWIYKW	EDRPDFLTVE	QRMRACYYSL
610	619
ASKVEGPPGS	TQKAEAACA

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-6054 Collapse assay details

Data source: Panorama

Official Gene Symbol: ZAP70
Peptide Sequence: TVYHYLISQDK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 207
Peptide End: 217

CPTAC ID: CPTAC-6054
Peptide Molecular Mass: 1,365.6929
Species: Human
Assay Type: Enrichment MRM
Enrichment Method: peptide immunoaffinity
Matrix: FFPE lung tumor tissue lysate pool
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Whiteaker JR, Zhao L, Schoenherr RM, Huang D, Lundeen RA, Voytovich U, Kennedy JJ, Ivey RG, Lin C, Murillo OD, Lorentzen TD, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Reading JJ, Perry CD, Richardson CW, Garcia-Buntley SS, Bocik W, Hewitt SM, Chowdhury S, Vandermeer J, Smith SD, Gopal AK, Ramchurren N, Fling SP, Wang P, Paulovich AG. A multiplexed assay for quantifying immunomodulatory proteins supports correlative studies in immunotherapy clinical trials. Front Oncol. 2023 May 2;13:1168710. doi: 10.3389/fonc.2023.1168710. PMID: 37205196; PMCID: PMC10185886.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: QTRAP 5500
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Eksigent nanoLC 425
Column Packing: 3 µm ChromXP C18EP, 120 Å
Column Dimensions: 150 x 0.075 mm
Flow Rate: 0.3 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b5 (1+)	32.9	24.4	34.7	35.7	25.7	32.6	48.5	35.4	47.6	15	15	15
b6 (1+)	48.8	15.6	33.9	49	17.5	31.3	69.2	23.4	46.1	15	15	15
b7 (1+)	56.3	16.1	41.6	61.5	26.5	49.4	83.4	31	64.6	15	15	15
y4 (1+)	37.9	10.8	37.8	36.6	18.4	42.2	52.7	21.3	56.7	15	15	15
y6 (1+)	30.7	17.5	28.4	43.5	19.4	30.7	53.2	26.1	41.8	15	15	15
y7 (1+)	31.5	16.1	29.6	32.5	13.7	30.5	45.3	21.1	42.5	15	15	15
y8 (1+)	40.7	12.4	29	42.3	16.5	26.8	58.7	20.6	39.5	15	15	15
sum	28.3	7.2	28.9	31	7.5	28.9	42	10.4	40.9	15	15	15

ZAP70

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-6054 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

Search form

ZAP70

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-6054 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments