SIGLEC7, CPTAC-6056 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
SIGLEC7	AIRM1

Sequence Length (AA)	Molecular Weight (Da)
467	51143

Protein Name
Sialic acid-binding Ig-like lectin 7

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MLLLLLLPLL	WGRERVEGQK	SNRKDYSLTM	QSSVTVQEGM	CVHVRCSFSY
60	70	80	90	100
PVDSQTDSDP	VHGYWFRAGN	DISWKAPVAT	NNPAWAVQEE	TRDRFHLLGD
110	120	130	140	150
PQTKNCTLSI	RDARMSDAGR	YFFRMEKGNI	KWNYKYDQLS	VNVTALTHRP
160	170	180	190	200
NILIPGTLES	GCFQNLTCSV	PWACEQGTPP	MISWMGTSVS	PLHPSTTRSS
210	220	230	240	250
VLTLIPQPQH	HGTSLTCQVT	LPGAGVTTNR	TIQLNVSYPP	QNLTVTVFQG
260	270	280	290	300
EGTASTALGN	SSSLSVLEGQ	SLRLVCAVDS	NPPARLSWTW	RSLTLYPSQP
310	320	330	340	350
SNPLVLELQV	HLGDEGEFTC	RAQNSLGSQH	VSLNLSLQQE	YTGKMRPVSG
360	370	380	390	400
VLLGAVGGAG	ATALVFLSFC	VIFIVVRSCR	KKSARPAADV	GDIGMKDANT
410	420	430	440	450
IRGSASQGNL	TESWADDNPR	HHGLAAHSSG	EEREIQYAPL	SFHKGEPQDL
460	467
SGQEATNNEY	SEIKIPK

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-6056 Collapse assay details

Data source: Panorama

Official Gene Symbol: SIGLEC7
Peptide Sequence: FHLLGDPQTK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 95
Peptide End: 104

CPTAC ID: CPTAC-6056
Peptide Molecular Mass: 1,154.6084
Species: Human
Assay Type: Enrichment MRM
Enrichment Method: peptide immunoaffinity
Matrix: FFPE lung tumor tissue lysate pool
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Whiteaker JR, Zhao L, Schoenherr RM, Huang D, Lundeen RA, Voytovich U, Kennedy JJ, Ivey RG, Lin C, Murillo OD, Lorentzen TD, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Reading JJ, Perry CD, Richardson CW, Garcia-Buntley SS, Bocik W, Hewitt SM, Chowdhury S, Vandermeer J, Smith SD, Gopal AK, Ramchurren N, Fling SP, Wang P, Paulovich AG. A multiplexed assay for quantifying immunomodulatory proteins supports correlative studies in immunotherapy clinical trials. Front Oncol. 2023 May 2;13:1168710. doi: 10.3389/fonc.2023.1168710. PMID: 37205196; PMCID: PMC10185886.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: QTRAP 5500
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Eksigent nanoLC 425
Column Packing: 3 µm ChromXP C18EP, 120 Å
Column Dimensions: 150 x 0.075 mm
Flow Rate: 0.3 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b3 (1+)	19.6	16.1	23.4	26.5	16	21.1	33	22.7	31.5	15	15	15
b4 (1+)	24.2	18.8	17.2	29.4	20.9	16.7	38.1	28.1	24	15	15	15
b5 (1+)	30.4	14.6	33.1	31.2	19.3	32.9	43.6	24.2	46.7	15	15	15
b6 (1+)	20.4	19.7	25.9	20.2	22.1	32.7	28.7	29.6	41.7	15	15	15
y4 (1+)	14.9	12.5	13.9	24.9	16.8	16	29	20.9	21.2	15	15	15
y6 (1+)	33.4	21.1	41.5	31.2	27.4	47.9	45.7	34.6	63.4	15	15	15
sum	15.7	10.1	12.2	19.4	15.2	13.6	25	18.2	18.3	15	15	15

SIGLEC7

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-6056 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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SIGLEC7

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-6056 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments