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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

LY75

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
LY75 CD205, CLEC13B
Sequence Length (AA) Molecular Weight (Da)
1722 198311
Protein Name
Lymphocyte antigen 75
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MRTGWATPRR PAGLLMLLFW FFDLAEPSGR AANDPFTIVH GNTGKCIKPV
60 70 80 90 100
YGWIVADDCD ETEDKLWKWV SQHRLFHLHS QKCLGLDITK SVNELRMFSC
110 120 130 140 150
DSSAMLWWKC EHHSLYGAAR YRLALKDGHG TAISNASDVW KKGGSEESLC
160 170 180 190 200
DQPYHEIYTR DGNSYGRPCE FPFLIDGTWH HDCILDEDHS GPWCATTLNY
210 220 230 240 250
EYDRKWGICL KPENGCEDNW EKNEQFGSCY QFNTQTALSW KEAYVSCQNQ
260 270 280 290 300
GADLLSINSA AELTYLKEKE GIAKIFWIGL NQLYSARGWE WSDHKPLNFL
310 320 330 340 350
NWDPDRPSAP TIGGSSCARM DAESGLWQSF SCEAQLPYVC RKPLNNTVEL
360 370 380 390 400
TDVWTYSDTR CDAGWLPNNG FCYLLVNESN SWDKAHAKCK AFSSDLISIH
410 420 430 440 450
SLADVEVVVT KLHNEDIKEE VWIGLKNINI PTLFQWSDGT EVTLTYWDEN
460 470 480 490 500
EPNVPYNKTP NCVSYLGELG QWKVQSCEEK LKYVCKRKGE KLNDASSDKM
510 520 530 540 550
CPPDEGWKRH GETCYKIYED EVPFGTNCNL TITSRFEQEY LNDLMKKYDK
560 570 580 590 600
SLRKYFWTGL RDVDSCGEYN WATVGGRRRA VTFSNWNFLE PASPGGCVAM
610 620 630 640 650
STGKSVGKWE VKDCRSFKAL SICKKMSGPL GPEEASPKPD DPCPEGWQSF
660 670 680 690 700
PASLSCYKVF HAERIVRKRN WEEAERFCQA LGAHLSSFSH VDEIKEFLHF
710 720 730 740 750
LTDQFSGQHW LWIGLNKRSP DLQGSWQWSD RTPVSTIIMP NEFQQDYDIR
760 770 780 790 800
DCAAVKVFHR PWRRGWHFYD DREFIYLRPF ACDTKLEWVC QIPKGRTPKT
810 820 830 840 850
PDWYNPDRAG IHGPPLIIEG SEYWFVADLH LNYEEAVLYC ASNHSFLATI
860 870 880 890 900
TSFVGLKAIK NKIANISGDG QKWWIRISEW PIDDHFTYSR YPWHRFPVTF
910 920 930 940 950
GEECLYMSAK TWLIDLGKPT DCSTKLPFIC EKYNVSSLEK YSPDSAAKVQ
960 970 980 990 1000
CSEQWIPFQN KCFLKIKPVS LTFSQASDTC HSYGGTLPSV LSQIEQDFIT
1010 1020 1030 1040 1050
SLLPDMEATL WIGLRWTAYE KINKWTDNRE LTYSNFHPLL VSGRLRIPEN
1060 1070 1080 1090 1100
FFEEESRYHC ALILNLQKSP FTGTWNFTSC SERHFVSLCQ KYSEVKSRQT
1110 1120 1130 1140 1150
LQNASETVKY LNNLYKIIPK TLTWHSAKRE CLKSNMQLVS ITDPYQQAFL
1160 1170 1180 1190 1200
SVQALLHNSS LWIGLFSQDD ELNFGWSDGK RLHFSRWAET NGQLEDCVVL
1210 1220 1230 1240 1250
DTDGFWKTVD CNDNQPGAIC YYSGNETEKE VKPVDSVKCP SPVLNTPWIP
1260 1270 1280 1290 1300
FQNCCYNFII TKNRHMATTQ DEVHTKCQKL NPKSHILSIR DEKENNFVLE
1310 1320 1330 1340 1350
QLLYFNYMAS WVMLGITYRN KSLMWFDKTP LSYTHWRAGR PTIKNEKFLA
1360 1370 1380 1390 1400
GLSTDGFWDI QTFKVIEEAV YFHQHSILAC KIEMVDYKEE YNTTLPQFMP
1410 1420 1430 1440 1450
YEDGIYSVIQ KKVTWYEALN MCSQSGGHLA SVHNQNGQLF LEDIVKRDGF
1460 1470 1480 1490 1500
PLWVGLSSHD GSESSFEWSD GSTFDYIPWK GQTSPGNCVL LDPKGTWKHE
1510 1520 1530 1540 1550
KCNSVKDGAI CYKPTKSKKL SRLTYSSRCP AAKENGSRWI QYKGHCYKSD
1560 1570 1580 1590 1600
QALHSFSEAK KLCSKHDHSA TIVSIKDEDE NKFVSRLMRE NNNITMRVWL
1610 1620 1630 1640 1650
GLSQHSVDQS WSWLDGSEVT FVKWENKSKS GVGRCSMLIA SNETWKKVEC
1660 1670 1680 1690 1700
EHGFGRVVCK VPLGPDYTAI AIIVATLSIL VLMGGLIWFL FQRHRLHLAG
1710 1720 1722
FSSVRYAQGV NEDEIMLPSF HD

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-6059 Collapse assay details

Data source: Panorama

Official Gene Symbol
LY75
Peptide Sequence
AANDPFTIVHGNTGK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
31
Peptide End
45
CPTAC ID
CPTAC-6059
Peptide Molecular Mass
1,540.7634
Species
Human
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
FFPE lung tumor tissue lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Whiteaker JR, Zhao L, Schoenherr RM, Huang D, Lundeen RA, Voytovich U, Kennedy JJ, Ivey RG, Lin C, Murillo OD, Lorentzen TD, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Reading JJ, Perry CD, Richardson CW, Garcia-Buntley SS, Bocik W, Hewitt SM, Chowdhury S, Vandermeer J, Smith SD, Gopal AK, Ramchurren N, Fling SP, Wang P, Paulovich AG. A multiplexed assay for quantifying immunomodulatory proteins supports correlative studies in immunotherapy clinical trials. Front Oncol. 2023 May 2;13:1168710. doi: 10.3389/fonc.2023.1168710. PMID: 37205196; PMCID: PMC10185886.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTRAP 5500
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Eksigent nanoLC 425
Column Packing
3 µm ChromXP C18EP, 120 Å
Column Dimensions
150 x 0.075 mm
Flow Rate
0.3 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 12.8 13.5 12.4 18.9 12.9 12.3 22.8 18.7 17.5 15 15 15
y7 (1+) 21.6 13.6 9.4 22.1 15.3 10.4 30.9 20.5 14 15 15 15
y8 (1+) 18.8 18.6 15 22.4 18.3 13.7 29.2 26.1 20.3 15 15 15
y9 (1+) 27.2 13.9 8.6 31.5 14 9.4 41.6 19.7 12.7 15 15 15
y10 (2+) 26.2 14.8 16.5 73.9 14.1 16 78.4 20.4 23 15 15 15
y11 (2+) 16.9 11.9 7.8 21.4 10 7.6 27.3 15.5 10.9 15 15 15
y12 (2+) 12.1 10.3 7.7 18.1 10.5 7.5 21.8 14.7 10.7 15 15 15
sum 14.9 10.5 7.2 20.1 9.3 6.9 25 14 10 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments