IFIT1, CPTAC-6065 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
IFIT1	G10P1, IFI56, IFNAI1, ISG56

Sequence Length (AA)	Molecular Weight (Da)
478	55360

Protein Name
Interferon-induced protein with tetratricopeptide repeats 1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MSTNGDDHQV	KDSLEQLRCH	FTWELSIDDD	EMPDLENRVL	DQIEFLDTKY
60	70	80	90	100
SVGIHNLLAY	VKHLKGQNEE	ALKSLKEAEN	LMQEEHDNQA	NVRSLVTWGN
110	120	130	140	150
FAWMYYHMGR	LAEAQTYLDK	VENICKKLSN	PFRYRMECPE	IDCEEGWALL
160	170	180	190	200
KCGGKNYERA	KACFEKVLEV	DPENPESSAG	YAISAYRLDG	FKLATKNHKP
210	220	230	240	250
FSLLPLRQAV	RLNPDNGYIK	VLLALKLQDE	GQEAEGEKYI	EEALANMSSQ
260	270	280	290	300
TYVFRYAAKF	YRRKGSVDKA	LELLKKALQE	TPTSVLLHHQ	IGLCYKAQMI
310	320	330	340	350
QIKEATKGQP	RGQNREKLDK	MIRSAIFHFE	SAVEKKPTFE	VAHLDLARMY
360	370	380	390	400
IEAGNHRKAE	ENFQKLLCMK	PVVEETMQDI	HFHYGRFQEF	QKKSDVNAII
410	420	430	440	450
HYLKAIKIEQ	ASLTRDKSIN	SLKKLVLRKL	RRKALDLESL	SLLGFVYKLE
460	470	478
GNMNEALEYY	ERALRLAADF	ENSVRQGP

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-6065 Collapse assay details

Data source: Panorama

Official Gene Symbol: IFIT1
Peptide Sequence: VLDQIEFLDTK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 39
Peptide End: 49

CPTAC ID: CPTAC-6065
Peptide Molecular Mass: 1,319.6973
Species: Human
Assay Type: Enrichment MRM
Enrichment Method: peptide immunoaffinity
Matrix: FFPE lung tumor tissue lysate pool
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Whiteaker JR, Zhao L, Schoenherr RM, Huang D, Lundeen RA, Voytovich U, Kennedy JJ, Ivey RG, Lin C, Murillo OD, Lorentzen TD, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Reading JJ, Perry CD, Richardson CW, Garcia-Buntley SS, Bocik W, Hewitt SM, Chowdhury S, Vandermeer J, Smith SD, Gopal AK, Ramchurren N, Fling SP, Wang P, Paulovich AG. A multiplexed assay for quantifying immunomodulatory proteins supports correlative studies in immunotherapy clinical trials. Front Oncol. 2023 May 2;13:1168710. doi: 10.3389/fonc.2023.1168710. PMID: 37205196; PMCID: PMC10185886.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: QTRAP 5500
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Eksigent nanoLC 425
Column Packing: 3 µm ChromXP C18EP, 120 Å
Column Dimensions: 150 x 0.075 mm
Flow Rate: 0.3 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b3 (1+)	13.8	17.2	11.1	20.4	18.6	13.9	24.6	25.3	17.8	15	15	15
b4 (1+)	19.3	18.1	7	23.9	20.8	8.3	30.7	27.6	10.9	15	15	15
b5 (1+)	26.7	18.3	14.1	28	32.4	22.1	38.7	37.2	26.2	15	15	15
y4 (1+)	19.3	20.6	14.2	26.7	20.7	16.4	32.9	29.2	21.7	15	15	15
y5 (1+)	22.8	18.3	9.1	30.2	21.9	8.2	37.8	28.5	12.2	15	15	15
y6 (1+)	17.3	13	6	18.9	17.3	12.5	25.6	21.6	13.9	15	15	15
y7 (1+)	12.6	13.4	6.3	16.2	17.3	10.7	20.5	21.9	12.4	15	15	15
sum	16.1	13.7	5.4	19.9	17.9	9.6	25.6	22.5	11	15	15	15

IFIT1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-6065 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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IFIT1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-6065 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments