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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

PMS1

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
PMS1 PMSL1
Sequence Length (AA) Molecular Weight (Da)
932 105830
Protein Name
PMS1 protein homolog 1
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MKQLPAATVR LLSSSQIITS VVSVVKELIE NSLDAGATSV DVKLENYGFD
60 70 80 90 100
KIEVRDNGEG IKAVDAPVMA MKYYTSKINS HEDLENLTTY GFRGEALGSI
110 120 130 140 150
CCIAEVLITT RTAADNFSTQ YVLDGSGHIL SQKPSHLGQG TTVTALRLFK
160 170 180 190 200
NLPVRKQFYS TAKKCKDEIK KIQDLLMSFG ILKPDLRIVF VHNKAVIWQK
210 220 230 240 250
SRVSDHKMAL MSVLGTAVMN NMESFQYHSE ESQIYLSGFL PKCDADHSFT
260 270 280 290 300
SLSTPERSFI FINSRPVHQK DILKLIRHHY NLKCLKESTR LYPVFFLKID
310 320 330 340 350
VPTADVDVNL TPDKSQVLLQ NKESVLIALE NLMTTCYGPL PSTNSYENNK
360 370 380 390 400
TDVSAADIVL SKTAETDVLF NKVESSGKNY SNVDTSVIPF QNDMHNDESG
410 420 430 440 450
KNTDDCLNHQ ISIGDFGYGH CSSEISNIDK NTKNAFQDIS MSNVSWENSQ
460 470 480 490 500
TEYSKTCFIS SVKHTQSENG NKDHIDESGE NEEEAGLENS SEISADEWSR
510 520 530 540 550
GNILKNSVGE NIEPVKILVP EKSLPCKVSN NNYPIPEQMN LNEDSCNKKS
560 570 580 590 600
NVIDNKSGKV TAYDLLSNRV IKKPMSASAL FVQDHRPQFL IENPKTSLED
610 620 630 640 650
ATLQIEELWK TLSEEEKLKY EEKATKDLER YNSQMKRAIE QESQMSLKDG
660 670 680 690 700
RKKIKPTSAW NLAQKHKLKT SLSNQPKLDE LLQSQIEKRR SQNIKMVQIP
710 720 730 740 750
FSMKNLKINF KKQNKVDLEE KDEPCLIHNL RFPDAWLMTS KTEVMLLNPY
760 770 780 790 800
RVEEALLFKR LLENHKLPAE PLEKPIMLTE SLFNGSHYLD VLYKMTADDQ
810 820 830 840 850
RYSGSTYLSD PRLTANGFKI KLIPGVSITE NYLEIEGMAN CLPFYGVADL
860 870 880 890 900
KEILNAILNR NAKEVYECRP RKVISYLEGE AVRLSRQLPM YLSKEDIQDI
910 920 930 932
IYRMKHQFGN EIKECVHGRP FFHHLTYLPE TT

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-6074 Collapse assay details

Data source: Panorama

Official Gene Symbol
PMS1
Peptide Sequence
LDELLQSQIEK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
678
Peptide End
688
CPTAC ID
CPTAC-6074
Peptide Molecular Mass
1,314.7031
Species
Human
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
FFPE lung tumor tissue lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Whiteaker JR, Zhao L, Schoenherr RM, Huang D, Lundeen RA, Voytovich U, Kennedy JJ, Ivey RG, Lin C, Murillo OD, Lorentzen TD, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Reading JJ, Perry CD, Richardson CW, Garcia-Buntley SS, Bocik W, Hewitt SM, Chowdhury S, Vandermeer J, Smith SD, Gopal AK, Ramchurren N, Fling SP, Wang P, Paulovich AG. A multiplexed assay for quantifying immunomodulatory proteins supports correlative studies in immunotherapy clinical trials. Front Oncol. 2023 May 2;13:1168710. doi: 10.3389/fonc.2023.1168710. PMID: 37205196; PMCID: PMC10185886.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTRAP 5500
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Eksigent nanoLC 425
Column Packing
3 µm ChromXP C18EP, 120 Å
Column Dimensions
150 x 0.075 mm
Flow Rate
0.3 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 16.5 4.9 9.1 19.8 8.4 7.4 25.8 9.7 11.7 15 15 15
b4 (1+) 14.2 8.3 8.2 17.9 8.8 6.8 22.8 12.1 10.7 15 15 15
y5 (1+) 20.9 8.2 9.7 21.9 10.3 10 30.3 13.2 13.9 15 15 15
y6 (1+) 16.9 10.9 8.2 17.1 11 7.9 24 15.5 11.4 15 15 15
y7 (1+) 18.4 9 7.1 19.6 11.2 5.2 26.9 14.4 8.8 15 15 15
y8 (1+) 22 7.7 5.6 22 9.8 8.9 31.1 12.5 10.5 15 15 15
sum 17.1 7.1 6.7 18.4 9 5.7 25.1 11.5 8.8 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments