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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
VEGFA VEGF
Sequence Length (AA) Molecular Weight (Da)
395 43597
Protein Name
Vascular endothelial growth factor A, long form
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MTDRQTDTAP SPSYHLLPGR RRTVDAAASR GQGPEPAPGG GVEGVGARGV
60 70 80 90 100
ALKLFVQLLG CSRFGGAVVR AGEAEPSGAA RSASSGREEP QPEEGEEEEE
110 120 130 140 150
KEEERGPQWR LGARKPGSWT GEAAVCADSA PAARAPQALA RASGRGGRVA
160 170 180 190 200
RRGAEESGPP HSPSRRGSAS RAGPGRASET MNFLLSWVHW SLALLLYLHH
210 220 230 240 250
AKWSQAAPMA EGGGQNHHEV VKFMDVYQRS YCHPIETLVD IFQEYPDEIE
260 270 280 290 300
YIFKPSCVPL MRCGGCCNDE GLECVPTEES NITMQIMRIK PHQGQHIGEM
310 320 330 340 350
SFLQHNKCEC RPKKDRARQE KKSVRGKGKG QKRKRKKSRY KSWSVPCGPC
360 370 380 390 395
SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-6210 Collapse assay details

Data source: Panorama

Official Gene Symbol
VEGFA
Peptide Sequence
FMDVYQR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
7
Peptide Start
223
Peptide End
229
CPTAC ID
CPTAC-6210
Peptide Molecular Mass
957.4378
Species
Homo Sapiens
Assay Type
Enrichment MRM
Enrichment Method
Peptide Immunoaffinity
Matrix
Frozen tissue
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTRAP 5500
Internal Standard
Extended heavy peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Eksigent
Column Packing
Reprosil C18, 3um
Column Dimensions
75µm x 15cm
Flow Rate
0.3 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 12.3 3.9 7.4 14.3 4 9.9 18.9 5.6 12.4 15 15 15
y5 (1+) 10.9 2.7 7.3 11.8 4.1 9.4 16.1 4.9 11.9 15 15 15
y4 (1+) 12.9 3.8 7.1 15.8 4.1 8.8 20.4 5.6 11.3 15 15 15
sum 11.1 2.6 7 12.4 3.8 9.2 16.6 4.6 11.6 15 15 15


Additional Resources and Comments