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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
GZMA CTLA3, HFSP
Sequence Length (AA) Molecular Weight (Da)
262 28999
Protein Name
Granzyme A
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MRNSYRFLAS SLSVVVSLLL IPEDVCEKII GGNEVTPHSR PYMVLLSLDR
60 70 80 90 100
KTICAGALIA KDWVLTAAHC NLNKRSQVIL GAHSITREEP TKQIMLVKKE
110 120 130 140 150
FPYPCYDPAT REGDLKLLQL MEKAKINKYV TILHLPKKGD DVKPGTMCQV
160 170 180 190 200
AGWGRTHNSA SWSDTLREVN ITIIDRKVCN DRNHYNFNPV IGMNMVCAGS
210 220 230 240 250
LRGGRDSCNG DSGSPLLCEG VFRGVTSFGL ENKCGDPRGP GVYILLSKKH
260 262
LNWIIMTIKG AV

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-6211 Collapse assay details

Data source: Panorama

Official Gene Symbol
GZMA
Peptide Modified Sequence
EFPYPC[+57.021464]YDPATR
Modification Type
Carbamidomethyl Cysteine
Protein - Site of Modification
N/A
Peptide - Site of Modification
7
Peptide Start
100
Peptide End
111
CPTAC ID
CPTAC-6211
Peptide Molecular Mass
1,514.6500
Species
Homo Sapiens
Assay Type
Enrichment MRM
Enrichment Method
Peptide Immunoaffinity
Matrix
Frozen tissue
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTRAP 5500
Internal Standard
Extended heavy peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Eksigent
Column Packing
Reprosil C18, 3um
Column Dimensions
75µm x 15cm
Flow Rate
0.3 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 7.1 2.4 5 6.1 4 8.8 9.4 4.7 10.1 15 15 15
y8 (1+) 8.7 3.7 4.4 6.7 3.9 6.7 11 5.4 8 15 15 15
y4 (1+) 8.1 2.5 4 7.3 4.7 6.7 10.9 5.3 7.8 15 15 15
sum 8.1 2.5 4 6.3 3.8 6.7 10.3 4.5 7.8 15 15 15


Additional Resources and Comments