NCAM1, CPTAC-6212 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
NCAM1	NCAM

Sequence Length (AA)	Molecular Weight (Da)
858	94574

Protein Name
Neural cell adhesion molecule 1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MLQTKDLIWT	LFFLGTAVSL	QVDIVPSQGE	ISVGESKFFL	CQVAGDAKDK
60	70	80	90	100
DISWFSPNGE	KLTPNQQRIS	VVWNDDSSST	LTIYNANIDD	AGIYKCVVTG
110	120	130	140	150
EDGSESEATV	NVKIFQKLMF	KNAPTPQEFR	EGEDAVIVCD	VVSSLPPTII
160	170	180	190	200
WKHKGRDVIL	KKDVRFIVLS	NNYLQIRGIK	KTDEGTYRCE	GRILARGEIN
210	220	230	240	250
FKDIQVIVNV	PPTIQARQNI	VNATANLGQS	VTLVCDAEGF	PEPTMSWTKD
260	270	280	290	300
GEQIEQEEDD	EKYIFSDDSS	QLTIKKVDKN	DEAEYICIAE	NKAGEQDATI
310	320	330	340	350
HLKVFAKPKI	TYVENQTAME	LEEQVTLTCE	ASGDPIPSIT	WRTSTRNISS
360	370	380	390	400
EEKASWTRPE	KQETLDGHMV	VRSHARVSSL	TLKSIQYTDA	GEYICTASNT
410	420	430	440	450
IGQDSQSMYL	EVQYAPKLQG	PVAVYTWEGN	QVNITCEVFA	YPSATISWFR
460	470	480	490	500
DGQLLPSSNY	SNIKIYNTPS	ASYLEVTPDS	ENDFGNYNCT	AVNRIGQESL
510	520	530	540	550
EFILVQADTP	SSPSIDQVEP	YSSTAQVQFD	EPEATGGVPI	LKYKAEWRAV
560	570	580	590	600
GEEVWHSKWY	DAKEASMEGI	VTIVGLKPET	TYAVRLAALN	GKGLGEISAA
610	620	630	640	650
SEFKTQPVQG	EPSAPKLEGQ	MGEDGNSIKV	NLIKQDDGGS	PIRHYLVRYR
660	670	680	690	700
ALSSEWKPEI	RLPSGSDHVM	LKSLDWNAEY	EVYVVAENQQ	GKSKAAHFVF
710	720	730	740	750
RTSAQPTAIP	ANGSPTSGLS	TGAIVGILIV	IFVLLLVVVD	ITCYFLNKCG
760	770	780	790	800
LFMCIAVNLC	GKAGPGAKGK	DMEEGKAAFS	KDESKEPIVE	VRTEEERTPN
810	820	830	840	850
HDGGKHTEPN	ETTPLTEPEK	GPVEAKPECQ	ETETKPAPAE	VKTVPNDATQ
858
TKENESKA

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-6212 Collapse assay details

Data source: Panorama

Official Gene Symbol: NCAM1
Peptide Sequence: GLGEISAASEFK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: 7
Peptide Start: 593
Peptide End: 604

CPTAC ID: CPTAC-6212
Peptide Molecular Mass: 1,207.6085
Species: Homo Sapiens
Assay Type: Enrichment MRM
Enrichment Method: Peptide Immunoaffinity
Matrix: Frozen tissue
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: QTRAP 5500
Internal Standard: Extended heavy peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Eksigent
Column Packing: Reprosil C18, 3um
Column Dimensions: 75µm x 15cm
Flow Rate: 0.3 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y6 (1+)	7.2	3.7	2.7	6.9	4.8	6.8	10	6.1	7.3	15	15	15
y8 (1+)	6.3	5.8	2.6	8.7	4.6	6.9	10.7	7.4	7.4	15	15	15
y10 (1+)	8.2	4.5	3.5	9.2	5	6.5	12.3	6.7	7.4	15	15	15
y7 (1+)	9.1	3.1	3	8.5	3.5	6	12.5	4.7	6.7	15	15	15
sum	7.6	3.5	2.8	8.2	3.2	6.3	11.2	4.7	6.9	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

View in CPTC Antibody Portal

Comments

Assay Details for non-CPTAC-3846 Collapse assay details

Data source: Panorama

Official Gene Symbol: Ncam1
Peptide Sequence: GLGEISAATEFK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 584
Peptide End: 595

CPTAC ID: non-CPTAC-3846
Peptide Molecular Mass: 1,221.6241
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.