Ncam1, CPTAC-3846 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
Ncam1	Ncam

Sequence Length (AA)	Molecular Weight (Da)
1115	119427

Protein Name
Neural cell adhesion molecule 1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MLRTKDLIWT	LFFLGTAVSL	QVDIVPSQGE	ISVGESKFFL	CQVAGDAKDK
60	70	80	90	100
DISWFSPNGE	KLSPNQQRIS	VVWNDDDSST	LTIYNANIDD	AGIYKCVVTA
110	120	130	140	150
EDGTQSEATV	NVKIFQKLMF	KNAPTPQEFK	EGEDAVIVCD	VVSSLPPTII
160	170	180	190	200
WKHKGRDVIL	KKDVRFIVLS	NNYLQIRGIK	KTDEGTYRCE	GRILARGEIN
210	220	230	240	250
FKDIQVIVNV	PPTVQARQSI	VNATANLGQS	VTLVCDADGF	PEPTMSWTKD
260	270	280	290	300
GEPIENEEED	DEKHIFSDDS	SELTIRNVDK	NDEAEYVCIA	ENKAGEQDAS
310	320	330	340	350
IHLKVFAKPK	ITYVENQTAM	ELEEQVTLTC	EASGDPIPSI	TWRTSTRNIS
360	370	380	390	400
SEEKTLDGHM	VVRSHARVSS	LTLKSIQYTD	AGEYICTASN	TIGQDSQSMY
410	420	430	440	450
LEFQYAPKLQ	GPVAVYTWEG	NQVNITCEVF	AYPSATISWF	RDGQLLPSSN
460	470	480	490	500
YSNIKIYNTP	SASYLEVTPD	SENDFGNYNC	TAVNRIGQES	LEFILVQADT
510	520	530	540	550
PSSPSIDRVE	PYSSTAQVQF	DEPEATGGVP	ILKYKAEWKS	LGEESWHFKW
560	570	580	590	600
YDAKEANMEG	IVTIMGLKPE	TRYSVRLAAL	NGKGLGEISA	ATEFKTQPVR
610	620	630	640	650
EPSAPKLEGQ	MGEDGNSIKV	NLIKQDDGGS	PIRHYLVKYR	ALASEWKPEI
660	670	680	690	700
RLPSGSDHVM	LKSLDWNAEY	EVYVVAENQQ	GKSKAAHFVF	RTSAQPTAIP
710	720	730	740	750
ANGSPTAGLS	TGAIVGILIV	IFVLLLVVMD	ITCYFLNKCG	LLMCIAVNLC
760	770	780	790	800
GKAGPGAKGK	DMEEGKAAFS	KDESKEPIVE	VRTEEERTPN	HDGGKHTEPN
810	820	830	840	850
ETTPLTEPEL	PADTTATVED	MLPSVTTVTT	NSDTITETFA	TAQNSPTSET
860	870	880	890	900
TTLTSSIAPP	ATTVPDSNSV	PAGQATPSKG	VTASSSSPAS	APKVAPLVDL
910	920	930	940	950
SDTPTSAPSA	SNLSSTVLAN	QGAVLSPSTP	ASAGETSKAP	PASKASPAPT
960	970	980	990	1000
PTPAGAASPL	AAVAAPATDA	PQAKQEAPST	KGPDPEPTQP	GTVKNPPEAA
1010	1020	1030	1040	1050
TAPASPKSKA	ATTNPSQGED	LKMDEGNFKT	PDIDLAKDVF	AALGSPRPAT
1060	1070	1080	1090	1100
GASGQASELA	PSPADSAVPP	APAKTEKGPV	ETKSEPPESE	AKPAPTEVKT
1110	1115
VPNDATQTKE	NESKA

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-6212 Collapse assay details

Data source: Panorama

Official Gene Symbol: NCAM1
Peptide Sequence: GLGEISAASEFK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: 7
Peptide Start: 593
Peptide End: 604

CPTAC ID: CPTAC-6212
Peptide Molecular Mass: 1,207.6085
Species: Homo Sapiens
Assay Type: Enrichment MRM
Enrichment Method: IMAC
Matrix: Frozen tissue
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: QTRAP 5500
Internal Standard: Extended heavy peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Eksigent
Column Packing: Reprosil C18, 3um
Column Dimensions: 75µm x 15cm
Flow Rate: 0.3 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y6 (1+)	7.2	3.7	2.7	6.9	4.8	6.8	10	6.1	7.3	15	15	15
y8 (1+)	6.3	5.8	2.6	8.7	4.6	6.9	10.7	7.4	7.4	15	15	15
y10 (1+)	8.2	4.5	3.5	9.2	5	6.5	12.3	6.7	7.4	15	15	15
y7 (1+)	9.1	3.1	3	8.5	3.5	6	12.5	4.7	6.7	15	15	15
sum	7.6	3.5	2.8	8.2	3.2	6.3	11.2	4.7	6.9	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

View in CPTC Antibody Portal

Comments

Assay Details for non-CPTAC-3846 Collapse assay details

Data source: Panorama

Official Gene Symbol: Ncam1
Peptide Sequence: GLGEISAATEFK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 584
Peptide End: 595

CPTAC ID: non-CPTAC-3846
Peptide Molecular Mass: 1,221.6241
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.