Mb, CPTAC-3847 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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Overview Data source: UniProt

Official Gene Symbol	Other Aliases
Mb	N/A

Sequence Length (AA)	Molecular Weight (Da)
154	17070

Protein Name
Myoglobin

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MGLSDGEWQL	VLNVWGKVEA	DLAGHGQEVL	IGLFKTHPET	LDKFDKFKNL
60	70	80	90	100
KSEEDMKGSE	DLKKHGCTVL	TALGTILKKK	GQHAAEIQPL	AQSHATKHKI
110	120	130	140	150
PVKYLEFISE	IIIEVLKKRH	SGDFGADAQG	AMSKALELFR	NDIAAKYKEL
154
GFQG

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-1474 Collapse assay details

Data source: Panorama

Official Gene Symbol: MB
Peptide Sequence: LFTGHPETLEK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 33
Peptide End: 43

CPTAC ID: CPTAC-1474
Peptide Molecular Mass: 1,270.6558
Species: Equus caballus (Horse)
Assay Type: Direct MRM
Matrix: depleted digested plasma
Submitting Laboratory: Broad Institute
Submitting Lab PI: Steven A. Carr

Publication

View Details (opens in a new window)

Large-Scale Interlaboratory Study to Develop, Analytically Validate and Apply Highly Multiplexed, Quantitative Peptide Assays to Measure Cancer-Relevant Proteins in Plasma. Abbatiello SE, Schilling B, Mani DR, Zimmerman LJ, Hall SC, MacLean B, Albertolle M, Allen S, Burgess M, Cusack MP, Gosh M, Hedrick V, Held JM, Inerowicz HD, Jackson A, Keshishian H, Kinsinger CR, Lyssand J, Makowski L, Mesri M, Rodriguez H, Rudnick P, Sadowski P, Sedransk N, Shaddox K, Skates SJ, Kuhn E, Smith D, Whiteaker JR, Whitwell C, Zhang S, Borchers CH, Fisher SJ, Gibson BW, Liebler DC, MacCoss MJ, Neubert TA, Paulovich AG, Regnier FE, Tempst P, Carr SA. Mol Cell Proteomics. 2015 Sep;14(9):2357-74. doi: 10.1074/mcp.M114.047050. Epub 2015 Feb 18. PMID: 25693799

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Quantiva TSQ
Internal Standard: heavy stable isotope peptide (delta 8-10)
Peptide Standard Purity: >95%
Peptide Standard Label Type: 15N

LC: Easy NanoLC1000
Column Packing: Reprosil C18, 1.9um, 200A
Column Dimensions: 0.075 x 150 mm
Flow Rate: 200 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y6 (1+)	2.4	3.5	3.3	3.2	7.2	6.3	4	8	7.1	15	15	15
y10 (2+)	4.7	4.1	2	3.6	6.1	5.1	5.9	7.3	5.5	15	15	15
y9 (2+)	3.5	2.6	2.1	4.8	6.1	3.8	5.9	6.6	4.3	15	15	15
sum	3	2.9	1.9	3.7	6.2	4.5	4.8	6.8	4.9	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

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Comments

Assay Details for non-CPTAC-3847 Collapse assay details

Data source: Panorama

Official Gene Symbol: Mb
Peptide Sequence: GQHAAEIQPLAQSHATK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 81
Peptide End: 97

CPTAC ID: non-CPTAC-3847
Peptide Molecular Mass: 1,785.9122
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers