TGFB1, CPTAC-6209 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
TGFB1	TGFB

Sequence Length (AA)	Molecular Weight (Da)
390	44325

Protein Name
Transforming growth factor beta-1 proprotein

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MPPSGLRLLP	LLLPLLWLLV	LTPGRPAAGL	STCKTIDMEL	VKRKRIEAIR
60	70	80	90	100
GQILSKLRLA	SPPSQGEVPP	GPLPEAVLAL	YNSTRDRVAG	ESAEPEPEPE
110	120	130	140	150
ADYYAKEVTR	VLMVETHNEI	YDKFKQSTHS	IYMFFNTSEL	REAVPEPVLL
160	170	180	190	200
SRAELRLLRL	KLKVEQHVEL	YQKYSNNSWR	YLSNRLLAPS	DSPEWLSFDV
210	220	230	240	250
TGVVRQWLSR	GGEIEGFRLS	AHCSCDSRDN	TLQVDINGFT	TGRRGDLATI
260	270	280	290	300
HGMNRPFLLL	MATPLERAQH	LQSSRHRRAL	DTNYCFSSTE	KNCCVRQLYI
310	320	330	340	350
DFRKDLGWKW	IHEPKGYHAN	FCLGPCPYIW	SLDTQYSKVL	ALYNQHNPGA
360	370	380	390
SAAPCCVPQA	LEPLPIVYYV	GRKPKVEQLS	NMIVRSCKCS

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-6209 Collapse assay details

Data source: Panorama

Official Gene Symbol: TGFB1
Peptide Sequence: EAVPEPVLLSR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: 7
Peptide Start: 142
Peptide End: 152

CPTAC ID: CPTAC-6209
Peptide Molecular Mass: 1,208.6765
Species: Homo Sapiens
Assay Type: Enrichment MRM
Enrichment Method: Peptide Immunoaffinity
Matrix: Frozen tissue
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: QTRAP 5500
Internal Standard: Extended heavy peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Eksigent
Column Packing: Reprosil C18, 3um
Column Dimensions: 75µm x 15cm
Flow Rate: 0.3 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y6 (1+)	8.2	3.5	5	7.5	4.6	7.2	11.1	5.8	8.8	15	15	15
y8 (1+)	6.1	4.3	5	11.5	4.4	7.9	13	6.2	9.3	15	15	15
y8 (2+)	6.6	4	4.1	7.9	4	7.8	10.3	5.7	8.8	15	15	15
sum	6.1	3.5	4.4	8.6	3.8	7.5	10.5	5.2	8.7	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

View in CPTC Antibody Portal

Comments

Assay Details for non-CPTAC-3952 Collapse assay details

Data source: Panorama

Official Gene Symbol: Tgfb1
Peptide Sequence: LHVEINGISPK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 232
Peptide End: 242

CPTAC ID: non-CPTAC-3952
Peptide Molecular Mass: 1,205.6768
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.