Tgfb1, CPTAC-3952 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
Tgfb1	N/A

Sequence Length (AA)	Molecular Weight (Da)
390	44310

Protein Name
Transforming growth factor beta-1 proprotein

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MPPSGLRLLP	LLLPLPWLLV	LTPGRPAAGL	STCKTIDMEL	VKRKRIEAIR
60	70	80	90	100
GQILSKLRLA	SPPSQGEVPP	GPLPEAVLAL	YNSTRDRVAG	ESADPEPEPE
110	120	130	140	150
ADYYAKEVTR	VLMVDRNNAI	YEKTKDISHS	IYMFFNTSDI	REAVPEPPLL
160	170	180	190	200
SRAELRLQRL	KSSVEQHVEL	YQKYSNNSWR	YLGNRLLTPT	DTPEWLSFDV
210	220	230	240	250
TGVVRQWLNQ	GDGIQGFRFS	AHCSCDSKDN	KLHVEINGIS	PKRRGDLGTI
260	270	280	290	300
HDMNRPFLLL	MATPLERAQH	LHSSRHRRAL	DTNYCFSSTE	KNCCVRQLYI
310	320	330	340	350
DFRKDLGWKW	IHEPKGYHAN	FCLGPCPYIW	SLDTQYSKVL	ALYNQHNPGA
360	370	380	390
SASPCCVPQA	LEPLPIVYYV	GRKPKVEQLS	NMIVRSCKCS

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node

to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-6209 Collapse assay details

Data source: Panorama

Official Gene Symbol: TGFB1
Peptide Sequence: EAVPEPVLLSR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: 7
Peptide Start: 142
Peptide End: 152

CPTAC ID: CPTAC-6209
Peptide Molecular Mass: 1,208.6765
Species: Homo Sapiens
Assay Type: Enrichment MRM
Enrichment Method: IMAC
Matrix: Frozen tissue
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: QTRAP 5500
Internal Standard: Extended heavy peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Eksigent
Column Packing: Reprosil C18, 3um
Column Dimensions: 75µm x 15cm
Flow Rate: 0.3 µL/min

Chromatograms

Data source: Panorama

view larger image

Response Curves

Data source: Panorama

view larger image

Retrieving Data

Repeatability

Data source: Panorama

view larger image

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y6 (1+)	8.2	3.5	5	7.5	4.6	7.2	11.1	5.8	8.8	15	15	15
y8 (1+)	6.1	4.3	5	11.5	4.4	7.9	13	6.2	9.3	15	15	15
y8 (2+)	6.6	4	4.1	7.9	4	7.8	10.3	5.7	8.8	15	15	15
sum	6.1	3.5	4.4	8.6	3.8	7.5	10.5	5.2	8.7	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

View in CPTC Antibody Portal

Comments

Assay Details for non-CPTAC-3952 Collapse assay details

Data source: Panorama

Official Gene Symbol: Tgfb1
Peptide Sequence: LHVEINGISPK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 232
Peptide End: 242

CPTAC ID: non-CPTAC-3952
Peptide Molecular Mass: 1,205.6768
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.