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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Tg

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Tg Tgn
Sequence Length (AA) Molecular Weight (Da)
2766 304473
Protein Name
Thyroglobulin
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MTALVLWVST LLSSVCLVAA NIFEYQVDAQ PLRPCELQRE KAFLKQAEYV
60 70 80 90 100
PQCSEDGSFQ TVQCQNDGQS CWCVDSDGRE VPGSRQLGRP TVCLSFCQLH
110 120 130 140 150
KQRILLGSYI NSTDALYLPQ CQDSGNYAPV QCDLQRVQCW CVDTEGMEVY
160 170 180 190 200
GTRQQGRPTR CPRSCEIRNR RLLHGVGDRS PPQCTADGEF MPVQCKFVNT
210 220 230 240 250
TDMMIFDLIH NYNRFPDAFV TFSSFRGRFP EVSGYCYCAD SQGRELAETG
260 270 280 290 300
LELLLDEIYD TIFAGLDQAS TFTQSTMYRI LQRRFLAIQL VISGRFRCPT
310 320 330 340 350
KCEVEQFAAT RFGHSYIPRC HRDGHYQTVQ CQTEGMCWCV DAQGREVPGT
360 370 380 390 400
RQQGQPPSCA ADQSCALERQ QALSRFYFET PDYFSPQDLL SSEDRLAPVS
410 420 430 440 450
GVRSDTSCPP RIKELFVDSG LLRSIAVEHY QRLSESRSLL REAIRAVFPS
460 470 480 490 500
RELAGLALQF TTNPKRLQQN LFGGTFLANA AQFNLSGALG TRSTFNFSQF
510 520 530 540 550
FQQFGLPGFL NRDRVTTLAK LLPVRLDSSS TPETLRVSEK TVAMNKRVVG
560 570 580 590 600
NFGFKVNLQE NQDALKFLVS LLELPEFLVF LQRAVSVPED IARDLGDVME
610 620 630 640 650
MVFSAQACKQ MPGKFFVPSC TAGGSYEDIQ CYAGECWCVD SRGKELDGSR
660 670 680 690 700
VRGGRPRCPT KCEKQRAQMQ SLASAQPAGS SFFVPTCTRE GYFLPVQCFN
710 720 730 740 750
SECYCVDTEG QVIPGTQSTV GEAKQCPSVC QLQAEQAFLG VVGVLLSNSS
760 770 780 790 800
MVPSISNVYI PQCSASGQWR HVQCDGPHEQ VFEWYERWKT QNGDGQELTP
810 820 830 840 850
AALLMKIVSY REVASRNFSL FLQSLYDAGQ QRIFPVLAQY PSLQDVPQVV
860 870 880 890 900
LEGATTPPGE NIFLDPYIFW QILNGQLSQY PGPYSDFNMP LEHFNLRSCW
910 920 930 940 950
CVDEAGQKLD GTQTKPGEIP ACPGPCEEVK LRVLKFIKET EEIVSASNAS
960 970 980 990 1000
SFPLGESFLV AKGIQLTSEE LDLPPQFPSR DAFSEKFLRG GEYAIRLAAQ
1010 1020 1030 1040 1050
STLTFYQSLR ASLGKSDGAA SLLWSGPYMP QCNMIGGWEP VQCHAGTGQC
1060 1070 1080 1090 1100
WCVDGRGEFI PGSLMSRSSQ MPQCPTNCEL SRASGLISAW KQAGPQRNPG
1110 1120 1130 1140 1150
PGDLFIPVCL QTGEYVRKQT SGTGTWCVDP ASGEGMPVNT NGSAQCPGLC
1160 1170 1180 1190 1200
DVLKSRALSR KVGLGYSPVC EALDGAFSPV QCDLAQGSCW CVLGSGEEVP
1210 1220 1230 1240 1250
GTRVVGTQPA CESPQCPLPF SGSDVADGVI FCETASSSGV TTVQQCQLLC
1260 1270 1280 1290 1300
RQGLRSAFSP GPLICSLESQ HWVTLPPPRA CQRPQLWQTM QTQAHFQLLL
1310 1320 1330 1340 1350
PPGKMCSVDY SGLLQAFQVF ILDELIARGF CQIQVKTFGT LVSSTVCDNS
1360 1370 1380 1390 1400
SIQVGCLTAE RLGVNVTWKL QLEDISVGSL PDLYSIERAV TGQDLLGRFA
1410 1420 1430 1440 1450
DLIQSGRFQL HLDSKTFSAD TTLYFLNGDS FVTSPRTQLG CMEGFYRVPT
1460 1470 1480 1490 1500
TRQDALGCVK CPEGSFSQDG RCTPCPAGTY QEQAGSSACI PCPRGRTTIT
1510 1520 1530 1540 1550
TGAFSKTHCV TDCQKNEAGL QCDQNGQYQA SQKNRDSGEV FCVDSEGRKL
1560 1570 1580 1590 1600
QWLQTEAGLS ESQCLMIRKF DKAPESKVIF DANSPVIVKS SVPSADSPLV
1610 1620 1630 1640 1650
QCLTDCANDE ACSFLTVSTM ESEVSCDFYS WTRDNFACVT SDQEQDAMGS
1660 1670 1680 1690 1700
LKATSFGSLR CQVKVRNSGK DSLAVYVKKG YESTAAGQKS FEPTGFQNVL
1710 1720 1730 1740 1750
SGLYSPVVFS ASGANLTDTH TYCLLACDND SCCDGFIITQ VKGGPTICGL
1760 1770 1780 1790 1800
LSSPDILLCH INDWRDTSAT QANATCAGVT YDQGSRQMTL SLGGQEFLQG
1810 1820 1830 1840 1850
LALLEGTQDS FTSFQQVYLW KDSDMGSRPE SMGCERGMVP RSDFPGDMAT
1860 1870 1880 1890 1900
ELFSPVDITQ VIVNTSHSLP SQQYWLFTHL FSAEQANLWC LSRCAQEPIF
1910 1920 1930 1940 1950
CQLADITKSS SLYFTCFLYP EAQVCDNVME SNAKNCSQIL PHQPTALFRR
1960 1970 1980 1990 2000
KVVLNDRVKN FYTRLPFQKL TGISIRDKVP MSGKLISNGF FECERLCDRD
2010 2020 2030 2040 2050
PCCTGFGFLN VSQLQGGEVT CLTLNSMGIQ TCNEESGATW RILDCGSEDT
2060 2070 2080 2090 2100
EVHTYPFGWY QKPAVWSDTP SFCPSAALQS LTEEKVTSDS WQTLALSSVI
2110 2120 2130 2140 2150
VDPSIKHFDV AHISTAATSN FSMAQDFCLQ QCSRHQDCLV TTLQIQPGVV
2160 2170 2180 2190 2200
RCVFYPDIQN CIHSLRSHTC WLLLHEEATY IYRKSGIPLV QSDVTSTPSV
2210 2220 2230 2240 2250
RIDSFGQLQG GSQVIKVGTA WKQVYRFLGV PYAAPPLADN RFRAPEVLNW
2260 2270 2280 2290 2300
TGSWDATKPR ASCWQPGTRT PTPPQINEDC LYLNVFVPEN LVSNASVLVF
2310 2320 2330 2340 2350
FHNTMEMEGS GGQLTIDGSI LAAVGNFIVV TANYRLGVFG FLSSGSDEVA
2360 2370 2380 2390 2400
GNWGLLDQVA ALTWVQSHIG AFGGDPQRVT LAADRSGADV ASIHLLISRP
2410 2420 2430 2440 2450
TRLQLFRKAL LMGGSALSPA AIISPERAQQ QAAALAKEVG CPTSSIQEVV
2460 2470 2480 2490 2500
SCLRQKPANI LNDAQTKLLA VSGPFHYWGP VVDGQYLREL PSRRLKRPLP
2510 2520 2530 2540 2550
VKVDLLIGGS QDDGLINRAK AVKQFEESQG RTNSKTAFYQ ALQNSLGGED
2560 2570 2580 2590 2600
SDARILAAAV WYYSLEHSTD DYASFSRALE NATRDYFIIC PMVNMASLWA
2610 2620 2630 2640 2650
RRTRGNVFMY HVPESYGHGS LELLADVQYA FGLPFYSAYQ GQFSTEEQSL
2660 2670 2680 2690 2700
SLKVMQYFSN FIRSGNPNYP HEFSRKAAEF ATPWPDFIPG AGGESYKELS
2710 2720 2730 2740 2750
AQLPNRQGLK QADCSFWSKY IQTLKDADGA KDAQLTKSEE EDLEVGPGLE
2760 2766
EDLSGSLEPV PKSYSK

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3953 Collapse assay details

Data source: Panorama

Official Gene Symbol
Tg
Peptide Sequence
FGHSYIPR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
312
Peptide End
319
CPTAC ID
non-CPTAC-3953
Peptide Molecular Mass
975.4927
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (2+) 6.2 1.9 3.7 10.2 12 13.4 11.9 12.1 13.9 15 15 14
y2 (1+) 3.2 3.8 4.5 7 11.3 12.6 7.7 11.9 13.4 15 15 14
y3 (1+) 5 2.7 4.2 8.4 10.9 15.9 9.8 11.2 16.4 15 15 14
b2 (1+) 117.6 33 29.6 115.5 27.4 29.4 164.8 42.9 41.7 15 15 14
y5 (1+) 4.5 3.2 4.2 7.1 12 16.2 8.4 12.4 16.7 15 15 14
sum 1.7 1.9 3.1 6.5 11.2 14 6.7 11.4 14.3 15 15 14

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments