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Overview Data source: UniProt

Official Gene Symbol Other Aliases
Thbs4 Tsp4
Sequence Length (AA) Molecular Weight (Da)
963 106366
Protein Name
Thrombospondin-4
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MPAPRAAAAA FLLLHLVLQP WQRTSAQATP QVFDLLPSSS QRLNPSALQP
60 70 80 90 100
VLTDPTLHEV YLISTFKLQS KSSATIFGLY SSSDNSKYFE FTVMGRLNKA
110 120 130 140 150
ILRYLKNDGK IHLVVFNNLQ LADGRRHRVL LRLSNLQRGD GSVELYLDCA
160 170 180 190 200
QADSVRNLPR AFSGLTQNPE SIELRTFQRK PQDFLEELKL VVRGSLFQVA
210 220 230 240 250
SLQDCFLQQS EPLAATSTGD FNRQFLGQMT QLNQLLGEVK DLLRQQVKET
260 270 280 290 300
SFLRNTIAEC QACGPLSFQS PTPNTLVPIA PPAPPTRPTR HCDSSPCFRG
310 320 330 340 350
VRCTDTRDGF QCGPCPDGYT GNGITCSDVD ECKYHPCYPG VRCVNLAPGF
360 370 380 390 400
RCDACPVGFT GPMVQGVGIN FAKTNKQVCT DVDECQNGAC VLNSICINTL
410 420 430 440 450
GSYRCGPCKP GYTGDQTRGC KTERSCRNPE QNPCSVHAQC IEERQGDVTC
460 470 480 490 500
VCGVGWAGDG YVCGKDVDID SYPDEELPCS ARNCKKDNCK YVPNSGQEDA
510 520 530 540 550
DRDGIGDACD EDADGDGILN EQDNCVLTHN IDQRNSDKDI FGDACDNCRM
560 570 580 590 600
VLNNDQKDTD GDGRGDACDD DMDGDGIKNI LDNCPRVPNR DQQDRDGDDV
610 620 630 640 650
GDACDSCPDV SNPNQSDVDN DLVGDSCDTN QDSDGDGHQD STDNCPTVIN
660 670 680 690 700
SSQLDTDKDG IGDECDDDDD NDGIPDLVPP GPDNCRLVPN PAQEDSNNDG
710 720 730 740 750
VGDICEADFD QDQVIDHIDV CPENAEITLT DFRAYQTVVL DPEGDAQIDP
760 770 780 790 800
NWVVLNQGME IVQTMNSDPG LAVGYTAFNG VDFEGTFHVN TQTDDDYAGF
810 820 830 840 850
IFGYQDSSSF YVVMWKQTEQ TYWQATPFRA VAEPGIQLKA VKSKTGPGEH
860 870 880 890 900
LRNSLWHTGD TSDQVRLLWK DSRNVGWKDK VSYRWFLQHR PQVGYIRVRF
910 920 930 940 950
YEGSELVADS GVTIDTTMRG GRLGVFCFSQ ENIIWSNLKY RCNDTIPEDF
960 963
QEFQTQSFDR LDN

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3954 Collapse assay details

Data source: Panorama

Official Gene Symbol
Thbs4
Peptide Sequence
AFSGLTQNPESIELR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
161
Peptide End
175
CPTAC ID
non-CPTAC-3954
Peptide Molecular Mass
1,660.8421
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 11.5 2.1 2.2 14.7 3.1 7.1 18.7 3.7 7.4 15 15 15
y8 (1+) 10.6 4.4 2.1 44.9 7.9 5.1 46.1 9 5.5 15 15 15
y7 (1+) 13.7 3.3 2 17.2 9.1 5 22 9.7 5.4 15 15 15
y2 (1+) 15.9 9.3 3.3 122.5 14.9 7.2 123.5 17.6 7.9 15 15 15
y7 (2+) 32.9 16.1 28.1 52.1 20.7 25.2 61.6 26.2 37.7 15 15 15
sum 7.4 2.7 1.2 50.2 7.5 5.4 50.7 8 5.5 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-2703 Collapse assay details

Data source: Panorama

Official Gene Symbol
THBS4
Peptide Sequence
KPQDFLEELK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
178
Peptide End
187
CPTAC ID
non-CPTAC-2703
Peptide Molecular Mass
1,245.6605
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b5 (1+) 8.5 4.9 9.5 18.1 9.9 15.2 20 11 17.9 15 15 15
y4 (1+) 6.5 4.3 10.7 13.2 8.8 15 14.7 9.8 18.4 15 15 15
b4 (1+) 7.9 7.4 10 11.3 9.4 12.4 13.8 12 15.9 15 15 15
sum 5.6 2.9 8.9 13.3 8 13.6 14.4 8.5 16.3 15 15 15


Additional Resources and Comments