THBS4, CPTAC-2703 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
THBS4	TSP4

Sequence Length (AA)	Molecular Weight (Da)
961	105869

Protein Name
Thrombospondin-4

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MLAPRGAAVL	LLHLVLQRWL	AAGAQATPQV	FDLLPSSSQR	LNPGALLPVL
60	70	80	90	100
TDPALNDLYV	ISTFKLQTKS	SATIFGLYSS	TDNSKYFEFT	VMGRLNKAIL
110	120	130	140	150
RYLKNDGKVH	LVVFNNLQLA	DGRRHRILLR	LSNLQRGAGS	LELYLDCIQV
160	170	180	190	200
DSVHNLPRAF	AGPSQKPETI	ELRTFQRKPQ	DFLEELKLVV	RGSLFQVASL
210	220	230	240	250
QDCFLQQSEP	LAATGTGDFN	RQFLGQMTQL	NQLLGEVKDL	LRQQVKETSF
260	270	280	290	300
LRNTIAECQA	CGPLKFQSPT	PSTVVPPAPP	APPTRPPRRC	DSNPCFRGVQ
310	320	330	340	350
CTDSRDGFQC	GPCPEGYTGN	GITCIDVDEC	KYHPCYPGVH	CINLSPGFRC
360	370	380	390	400
DACPVGFTGP	MVQGVGISFA	KSNKQVCTDI	DECRNGACVP	NSICVNTLGS
410	420	430	440	450
YRCGPCKPGY	TGDQIRGCKA	ERNCRNPELN	PCSVNAQCIE	ERQGDVTCVC
460	470	480	490	500
GVGWAGDGYI	CGKDVDIDSY	PDEELPCSAR	NCKKDNCKYV	PNSGQEDADR
510	520	530	540	550
DGIGDACDED	ADGDGILNEQ	DNCVLIHNVD	QRNSDKDIFG	DACDNCLSVL
560	570	580	590	600
NNDQKDTDGD	GRGDACDDDM	DGDGIKNILD	NCPKFPNRDQ	RDKDGDGVGD
610	620	630	640	650
ACDSCPDVSN	PNQSDVDNDL	VGDSCDTNQD	SDGDGHQDST	DNCPTVINSA
660	670	680	690	700
QLDTDKDGIG	DECDDDDDND	GIPDLVPPGP	DNCRLVPNPA	QEDSNSDGVG
710	720	730	740	750
DICESDFDQD	QVIDRIDVCP	ENAEVTLTDF	RAYQTVVLDP	EGDAQIDPNW
760	770	780	790	800
VVLNQGMEIV	QTMNSDPGLA	VGYTAFNGVD	FEGTFHVNTQ	TDDDYAGFIF
810	820	830	840	850
GYQDSSSFYV	VMWKQTEQTY	WQATPFRAVA	EPGIQLKAVK	SKTGPGEHLR
860	870	880	890	900
NSLWHTGDTS	DQVRLLWKDS	RNVGWKDKVS	YRWFLQHRPQ	VGYIRVRFYE
910	920	930	940	950
GSELVADSGV	TIDTTMRGGR	LGVFCFSQEN	IIWSNLKYRC	NDTIPEDFQE
960	961
FQTQNFDRFD	N

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt

Assay Details for non-CPTAC-3954 Collapse assay details

Data source: Panorama

Official Gene Symbol: Thbs4
Peptide Sequence: AFSGLTQNPESIELR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 161
Peptide End: 175

CPTAC ID: non-CPTAC-3954
Peptide Molecular Mass: 1,660.8421
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >80%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y10 (1+)	11.5	2.1	2.2	14.7	3.1	7.1	18.7	3.7	7.4	15	15	15
y8 (1+)	10.6	4.4	2.1	44.9	7.9	5.1	46.1	9	5.5	15	15	15
y7 (1+)	13.7	3.3	2	17.2	9.1	5	22	9.7	5.4	15	15	15
y2 (1+)	15.9	9.3	3.3	122.5	14.9	7.2	123.5	17.6	7.9	15	15	15
y7 (2+)	32.9	16.1	28.1	52.1	20.7	25.2	61.6	26.2	37.7	15	15	15
sum	7.4	2.7	1.2	50.2	7.5	5.4	50.7	8	5.5	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for non-CPTAC-2703 Collapse assay details

Data source: Panorama

Official Gene Symbol: THBS4
Peptide Sequence: KPQDFLEELK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 178
Peptide End: 187

CPTAC ID: non-CPTAC-2703
Peptide Molecular Mass: 1,245.6605
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers