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Overview Data source: UniProt

Official Gene Symbol Other Aliases
MAP2K2 MEK2,MKK2,PRKMK2
Sequence Length (AA) Molecular Weight (Da)
400 44424
Protein Name
Dual specificity mitogen-activated protein kinase kinase 2
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MLARRKPVLP ALTINPTIAE GPSPTSEGAS EANLVDLQKK LEELELDEQQ
60 70 80 90 100
KKRLEAFLTQ KAKVGELKDD DFERISELGA GNGGVVTKVQ HRPSGLIMAR
110 120 130 140 150
KLIHLEIKPA IRNQIIRELQ VLHECNSPYI VGFYGAFYSD GEISICMEHM
160 170 180 190 200
DGGSLDQVLK EAKRIPEEIL GKVSIAVLRG LAYLREKHQI MHRDVKPSNI
210 220 230 240 250
LVNSRGEIKL CDFGVSGQLI DSMANSFVGT RSYMAPERLQ GTHYSVQSDI
260 270 280 290 300
WSMGLSLVEL AVGRYPIPPP DAKELEAIFG RPVVDGEEGE PHSISPRPRP
310 320 330 340 350
PGRPVSGHGM DSRPAMAIFE LLDYIVNEPP PKLPNGVFTP DFQEFVNKCL
360 370 380 390 400
IKNPAERADL KMLTNHTFIK RSEVEEVDFA GWLCKTLRLN QPGTPTRTAV

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-811 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP2K2
Peptide Sequence
LNQPGTPTR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
389
Peptide End
397
CPTAC ID
CPTAC-811
Peptide Molecular Mass
982.5196
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 5.3 2.8 2.6 5.2 2.8 2.9 7.4 4 3.9 15 15 15
y5 (1+) 6.6 2.1 5.1 6.5 2.3 5.5 9.3 3.1 7.5 15 15 15
y6 (1+) 1.2 1.1 1.7 1.6 1 1.8 2 1.5 2.5 15 15 15
y7 (1+) 6.6 4.1 4.4 6.4 3.5 5.5 9.2 5.4 7 15 15 15
b3 (1+) 6.4 3.9 3.5 10.3 5.3 3.5 12.1 6.6 4.9 15 15 15
sum 1.4 0.8 1.3 1.7 0.8 1.4 2.2 1.1 1.9 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-1045 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP2K2
Peptide Modified Sequence
LNQPGTPT[+80.0]R
Modification Type
Phospho (ST)
Protein - Site of Modification
396
Peptide - Site of Modification
8
Peptide Start
389
Peptide End
397
CPTAC ID
CPTAC-1045
Peptide Molecular Mass
1,062.4859
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 7.9 9.4 6.1 10.3 10 6.3 13 13.7 8.8 15 15 15
y3 (1+) 42.7 23.4 14.6 43.2 22.8 18.2 60.7 32.7 23.3 15 15 15
b3 (1+) 27 10.4 10.7 26.9 11 12.3 38.1 15.1 16.3 15 15 15
sum 8.8 9.2 4.1 8.1 9.2 5.1 12 13 6.5 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-812 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP2K2
Peptide Sequence
LPNGVFTPDFQEFVNK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
333
Peptide End
348
CPTAC ID
CPTAC-812
Peptide Molecular Mass
1,850.9203
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 22.4 8.9 10.8 24.4 11.6 11.4 33.1 14.6 15.7 15 15 15
y4 (1+) 28.3 11.4 7.4 27.8 11.1 8.5 39.7 15.9 11.3 15 15 15
y5 (1+) 29.4 12.1 9.5 25.6 21.5 13.2 39 24.7 16.3 15 15 15
y7 (1+) 15.9 12.5 10.6 17.1 11.3 10.9 23.3 16.9 15.2 15 15 15
y9 (1+) 15.9 10.1 7.3 16.1 8.5 8 22.6 13.2 10.8 15 15 15
y10 (1+) 21.6 11.3 6.8 25.9 11.5 7.4 33.7 16.1 10 15 15 15
y11 (1+) 14.4 10 10.6 16.1 10.1 10.8 21.6 14.2 15.1 15 15 15
b9 (1+) 22.5 15.3 11.5 27.7 17.3 12.6 35.7 23.1 17.1 15 15 15
b7 (1+) 25.8 29.7 11.5 39.6 25.8 15 47.3 39.3 18.9 15 15 15
b5 (1+) 22.8 19.9 11.2 24.2 23.7 14.3 33.2 30.9 18.2 15 15 15
b4 (1+) 16.9 8.5 11 21.7 11.2 9.9 27.5 14.1 14.8 15 15 15
sum 2.8 4.7 3.2 4.2 4.1 3.2 5 6.2 4.5 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-813 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP2K2
Peptide Sequence
SYMAPER
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
232
Peptide End
238
CPTAC ID
CPTAC-813
Peptide Molecular Mass
852.3800
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 16.3 7.1 6.4 11.2 6.7 5.7 19.8 9.8 8.6 15 15 15
y4 (1+) 7.7 5.8 4.5 8 6.7 3.8 11.1 8.9 5.9 15 15 15
y5 (1+) 6.8 4.3 3.4 6.1 4.2 4.4 9.1 6 5.6 15 15 15
sum 6.2 2.7 3.2 4.5 2.5 3.7 7.7 3.7 4.9 15 15 15

Additional Resources and Comments