Overview Data source: UniProt

Official Gene Symbol Other Aliases
MAPK8 JNK1,PRKM8,SAPK1,SAPK1C
Sequence Length (AA) Molecular Weight (Da)
427 48296
Protein Name
Mitogen-activated protein kinase 8
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MSRSKRDNNF YSVEIGDSTF TVLKRYQNLK PIGSGAQGIV CAAYDAILER
60 70 80 90 100
NVAIKKLSRP FQNQTHAKRA YRELVLMKCV NHKNIIGLLN VFTPQKSLEE
110 120 130 140 150
FQDVYIVMEL MDANLCQVIQ MELDHERMSY LLYQMLCGIK HLHSAGIIHR
160 170 180 190 200
DLKPSNIVVK SDCTLKILDF GLARTAGTSF MMTPYVVTRY YRAPEVILGM
210 220 230 240 250
GYKENVDLWS VGCIMGEMVC HKILFPGRDY IDQWNKVIEQ LGTPCPEFMK
260 270 280 290 300
KLQPTVRTYV ENRPKYAGYS FEKLFPDVLF PADSEHNKLK ASQARDLLSK
310 320 330 340 350
MLVIDASKRI SVDEALQHPY INVWYDPSEA EAPPPKIPDK QLDEREHTIE
360 370 380 390 400
EWKELIYKEV MDLEERTKNG VIRGQPSPLG AAVINGSQHP SSSSSVNDVS
410 420 427
SMSTDPTLAS DTDSSLEAAA GPLGCCR

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
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Assay Details for CPTAC-889 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAPK8
Peptide Sequence
EVMDLEER
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
359
Peptide End
366
CPTAC ID
CPTAC-889
Peptide Molecular Mass
1,019.4594
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 9.3 4.7 4.6 8.3 5.3 4.7 12.5 7.1 6.6 15 15 15
y4 (1+) 4.9 2.9 2.4 5.6 3 2 7.4 4.2 3.1 15 15 15
y5 (1+) 6.4 3.1 1.9 5.6 3.7 2.2 8.5 4.8 2.9 15 15 15
y6 (1+) 5.8 2.4 2.6 6.2 2.8 2.9 8.5 3.7 3.9 15 15 15
sum 2.3 1.9 1.2 3 2.1 1.9 3.8 2.8 2.2 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-890 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAPK8
Peptide Sequence
NIIGLLNVFTPQK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
84
Peptide End
96
CPTAC ID
CPTAC-890
Peptide Molecular Mass
1,455.8450
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 18.9 5.6 3.4 20.1 7 3.4 27.6 9 4.8 15 15 15
y4 (1+) 26.3 13.1 7.1 26.8 14.3 6.8 37.5 19.4 9.8 15 15 15
y5 (1+) 31.2 9.6 7.6 29.5 10.6 10 42.9 14.3 12.6 15 15 15
y6 (1+) 22.7 15.1 14.9 26.6 13.6 17.5 35 20.3 23 15 15 15
y7 (1+) 26.5 7.3 10.7 26.6 11.6 11.1 37.5 13.7 15.4 15 15 15
y8 (1+) 33.3 12.5 8.8 30.3 15 8.8 45 19.5 12.4 15 15 15
y10 (1+) 16.3 4.1 6.2 12.4 10 9.5 20.5 10.8 11.3 15 15 15
y11 (1+) 26.5 11.9 8.1 31.6 9.6 9.8 41.2 15.3 12.7 15 15 15
b4 (1+) 30.7 13.8 8 31.6 14 8.3 44.1 19.7 11.5 15 15 15
b3 (1+) 25.7 8.9 15 29.3 13.3 15.1 39 16 21.3 15 15 15
sum 8.2 3 2.1 9 3.3 1.9 12.2 4.5 2.8 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-891 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAPK8
Peptide Modified Sequence
TAGTSFMMTPY[+80.0]VVTR
Modification Type
Phospho (Y)
Protein - Site of Modification
185
Peptide - Site of Modification
11
Peptide Start
175
Peptide End
189
CPTAC ID
CPTAC-891
Peptide Molecular Mass
1,740.7616
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 28 14.6 17.7 33.6 13.5 16.8 43.7 19.9 24.4 15 15 15
y6 (1+) 18.1 6.3 6.5 18.1 6.6 5.1 25.6 9.1 8.3 15 15 15
y7 (1+) 17.8 11 4.4 18.6 9.9 6 25.7 14.8 7.4 15 15 15
y8 (1+) 21.4 11.9 6.5 18.6 12.4 6 28.4 17.2 8.8 15 15 15
y9 (1+) 15.5 7.7 8.2 16.9 6.9 6.8 22.9 10.3 10.7 15 15 15
y11 (1+) 55.4 10 14.3 56.6 15.4 9.3 79.2 18.4 17.1 15 15 15
b3 (1+) 34.7 27 15.8 34.7 29 18.9 49.1 39.6 24.6 15 15 15
sum 10.3 3.7 1.5 10.8 5 2.7 14.9 6.2 3.1 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-892 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAPK8
Peptide Sequence
TAGTSFMMTPYVVTR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
175
Peptide End
189
CPTAC ID
CPTAC-892
Peptide Molecular Mass
1,660.7953
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 17 12.3 13.1 22.6 11.7 15.3 28.3 17 20.1 15 15 15
y6 (1+) 6.1 4.1 3.9 7.1 4.6 4.7 9.4 6.2 6.1 15 15 15
y7 (1+) 7.4 6.1 7.1 11.9 5.4 9.1 14 8.1 11.5 15 15 15
y8 (1+) 7.5 3.8 4.3 9.9 3.5 4.1 12.4 5.2 5.9 15 15 15
y9 (1+) 7.4 6.5 2.8 6.7 6.2 3.4 10 9 4.4 15 15 15
y10 (1+) 9.4 6.4 5.8 14.5 6.7 6.3 17.3 9.3 8.6 15 15 15
y11 (1+) 7.9 10.1 5 12.7 9.4 5.5 15 13.8 7.4 15 15 15
b3 (1+) 15.2 11.8 10.3 16 13.2 14.9 22.1 17.7 18.1 15 15 15
sum 3.8 2 2.5 6.5 1.9 2.9 7.5 2.8 3.8 15 15 15

Additional Resources and Comments