MAPK8, CPTAC-5706 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
MAPK8	JNK1, PRKM8, SAPK1, SAPK1C

Sequence Length (AA)	Molecular Weight (Da)
427	48296

Protein Name
Mitogen-activated protein kinase 8

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MSRSKRDNNF	YSVEIGDSTF	TVLKRYQNLK	PIGSGAQGIV	CAAYDAILER
60	70	80	90	100
NVAIKKLSRP	FQNQTHAKRA	YRELVLMKCV	NHKNIIGLLN	VFTPQKSLEE
110	120	130	140	150
FQDVYIVMEL	MDANLCQVIQ	MELDHERMSY	LLYQMLCGIK	HLHSAGIIHR
160	170	180	190	200
DLKPSNIVVK	SDCTLKILDF	GLARTAGTSF	MMTPYVVTRY	YRAPEVILGM
210	220	230	240	250
GYKENVDLWS	VGCIMGEMVC	HKILFPGRDY	IDQWNKVIEQ	LGTPCPEFMK
260	270	280	290	300
KLQPTVRTYV	ENRPKYAGYS	FEKLFPDVLF	PADSEHNKLK	ASQARDLLSK
310	320	330	340	350
MLVIDASKRI	SVDEALQHPY	INVWYDPSEA	EAPPPKIPDK	QLDEREHTIE
360	370	380	390	400
EWKELIYKEV	MDLEERTKNG	VIRGQPSPLG	AAVINGSQHP	SSSSSVNDVS
410	420	427
SMSTDPTLAS	DTDSSLEAAA	GPLGCCR

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-5792 Collapse assay details

Data source: Panorama

Official Gene Symbol: MAPK8
Peptide Modified Sequence: TAGTSFMMTPY[+79.966331]VVTR
Modification Type: Phospho (Y)
Protein - Site of Modification: N/A
Peptide - Site of Modification: 3
Peptide Start: 175
Peptide End: 189

CPTAC ID: CPTAC-5792
Peptide Molecular Mass: 1,740.7616
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM
Enrichment Method: IMAC
Matrix: Cell Lysate
Submitting Laboratory: Fred Hutchinson Cancer Research Center, H. Lee Moffitt Cancer Center & Research Institute, Broad Institute
Submitting Lab PI: Amanda Paulovich, John Koomen, Steven A. Carr

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: SCIEX 5500 / Thermo Quantiva
Internal Standard: Stable Isotope Labeled Standard
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Dionex Ultimate 3000
Column Packing: C18 PepMap 100
Column Dimensions: 75 micron ID x 25 cm length
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b9 (1+)	18.5	18	23.5	24.2	25.5	28.1	30.5	31.2	36.6	16	15	16
y6 (1+)	11.8	11.6	13.6	23.1	18.8	19	25.9	22.1	23.4	16	15	16
sum	10.9	10.9	13.4	22.7	18.6	18.7	25.2	21.6	23	16	15	16

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y10 (1+)	13.2	5.5	3.8	12.7	5.6	3.9	18.3	7.8	5.4	15	15	15
y6 (1+)	4.9	2.7	1.9	4.9	3.7	2	6.9	4.6	2.8	15	15	15
y7 (1+)	6.7	4.7	2.1	6.6	5.3	2.7	9.4	7.1	3.4	15	15	15
y8 (1+)	4.9	3.3	2.3	6	4	2.4	7.7	5.2	3.3	15	15	15
y9 (1+)	8.8	2.8	2.7	8.3	4.2	2.9	12.1	5	4	15	15	15
sum	2.9	2	1.2	2.8	3.3	1.4	4	3.9	1.8	15	15	15

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y3 (1+)	21.9	5.7	4	25.1	7.7	6.9	33.3	9.6	8	15	15	15
y9-98 (1+)	20.6	15.5	8.5	23.7	18.4	14.1	31.4	24.1	16.5	15	15	15
y8-98 (1+)	21.8	8.4	4.5	23.6	9.1	5.2	32.1	12.4	6.9	15	15	15
y7 (1+)	33.8	35	12.2	35.1	52.6	13.1	48.7	63.2	17.9	15	15	15
y6 (1+)	7.6	4.6	3.1	14.8	5.3	5.4	16.6	7	6.2	15	15	15
sum	9.8	2.4	3.3	13.8	3.6	5.9	16.9	4.3	6.8	15	15	15

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y10 (1+)	11.5	14.3	7.2	10.5	17.1	7.4	15.6	22.3	10.3	15	15	15
y9 (1+)	9.7	8.7	4.3	14.6	13.2	4.2	17.5	15.8	6	15	15	15
y8 (1+)	8.9	10	6.8	18.1	12.1	7.3	20.2	15.7	10	15	15	15
y7 (1+)	11.1	7.6	3.4	15.7	9	3.5	19.2	11.8	4.9	15	15	15
y6 (1+)	5.7	5	3.7	8.7	6.6	4	10.4	8.3	5.4	15	15	15
sum	3.9	4.5	2.4	7.6	5.7	2.9	8.5	7.3	3.8	15	15	15

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y3 (1+)	9.3	4.7	4.6	8.3	5.3	4.7	12.5	7.1	6.6	15	15	15
y4 (1+)	4.9	2.9	2.4	5.6	3	2	7.4	4.2	3.1	15	15	15
y5 (1+)	6.4	3.1	1.9	5.6	3.7	2.2	8.5	4.8	2.9	15	15	15
y6 (1+)	5.8	2.4	2.6	6.2	2.8	2.9	8.5	3.7	3.9	15	15	15
sum	2.3	1.9	1.2	3	2.1	1.9	3.8	2.8	2.2	15	15	15

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MAPK8

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-5792 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

Assay Details for non-CPTAC-5407 Collapse assay details

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Assay Parameters Collapse assay parameters

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Response Curves

Repeatability

Additional Resources and Comments

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Assay Details for non-CPTAC-5706 Collapse assay details

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Assay Parameters Collapse assay parameters

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Repeatability

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Assay Details for non-CPTAC-5707 Collapse assay details

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Assay Details for CPTAC-889 Collapse assay details

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Assay Details for CPTAC-3145 Collapse assay details

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Assay Details for CPTAC-890 Collapse assay details

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Assay Details for CPTAC-3144 Collapse assay details

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Assay Details for CPTAC-891 Collapse assay details

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Assay Details for CPTAC-892 Collapse assay details

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