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Overview Data source: UniProt

Official Gene Symbol Other Aliases
MAPK8 JNK1, PRKM8, SAPK1, SAPK1C
Sequence Length (AA) Molecular Weight (Da)
427 48296
Protein Name
Mitogen-activated protein kinase 8
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MSRSKRDNNF YSVEIGDSTF TVLKRYQNLK PIGSGAQGIV CAAYDAILER
60 70 80 90 100
NVAIKKLSRP FQNQTHAKRA YRELVLMKCV NHKNIIGLLN VFTPQKSLEE
110 120 130 140 150
FQDVYIVMEL MDANLCQVIQ MELDHERMSY LLYQMLCGIK HLHSAGIIHR
160 170 180 190 200
DLKPSNIVVK SDCTLKILDF GLARTAGTSF MMTPYVVTRY YRAPEVILGM
210 220 230 240 250
GYKENVDLWS VGCIMGEMVC HKILFPGRDY IDQWNKVIEQ LGTPCPEFMK
260 270 280 290 300
KLQPTVRTYV ENRPKYAGYS FEKLFPDVLF PADSEHNKLK ASQARDLLSK
310 320 330 340 350
MLVIDASKRI SVDEALQHPY INVWYDPSEA EAPPPKIPDK QLDEREHTIE
360 370 380 390 400
EWKELIYKEV MDLEERTKNG VIRGQPSPLG AAVINGSQHP SSSSSVNDVS
410 420 427
SMSTDPTLAS DTDSSLEAAA GPLGCCR

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-5792 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAPK8
Peptide Modified Sequence
TAGTSFMMTPY[+79.966331]VVTR
Modification Type
Phospho (Y)
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
175
Peptide End
189
CPTAC ID
CPTAC-5792
Peptide Molecular Mass
1,740.7616
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
N/A
Matrix
Cell Lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center, H. Lee Moffitt Cancer Center & Research Institute, Broad Institute
Submitting Lab PI
Amanda Paulovich, John Koomen, Steven A. Carr

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
SCIEX 5500 / Thermo Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Dionex Ultimate 3000
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b9 (1+) 18.5 18 23.5 24.2 25.5 28.1 30.5 31.2 36.6 16 15 16
y6 (1+) 11.8 11.6 13.6 23.1 18.8 19 25.9 22.1 23.4 16 15 16
sum 10.9 10.9 13.4 22.7 18.6 18.7 25.2 21.6 23 16 15 16


Additional Resources and Comments


Assay Details for non-CPTAC-5407 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAPK8
Peptide Sequence
TAGTSFMMTPYVVTR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
175
Peptide End
189
CPTAC ID
non-CPTAC-5407
Peptide Molecular Mass
1,660.7953
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Cell Lysate
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
RSLCnano and Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
RSLCnano
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nl/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 13.2 5.5 3.8 12.7 5.6 3.9 18.3 7.8 5.4 15 15 15
y6 (1+) 4.9 2.7 1.9 4.9 3.7 2 6.9 4.6 2.8 15 15 15
y7 (1+) 6.7 4.7 2.1 6.6 5.3 2.7 9.4 7.1 3.4 15 15 15
y8 (1+) 4.9 3.3 2.3 6 4 2.4 7.7 5.2 3.3 15 15 15
y9 (1+) 8.8 2.8 2.7 8.3 4.2 2.9 12.1 5 4 15 15 15
sum 2.9 2 1.2 2.8 3.3 1.4 4 3.9 1.8 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-5706 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAPK8
Peptide Modified Sequence
TAGTSFMMT[+79.966331]PYVVTR
Modification Type
Phospho (ST)
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
175
Peptide End
189
CPTAC ID
non-CPTAC-5706
Peptide Molecular Mass
1,740.7616
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
N/A
Matrix
Cell Lysate
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Thermo Quantiva
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Dionex Ultimate 3000 RSLCnano
Column Packing
Pepmap 100
Column Dimensions
75um x 25cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 21.9 5.7 4 25.1 7.7 6.9 33.3 9.6 8 15 15 15
y9-98 (1+) 20.6 15.5 8.5 23.7 18.4 14.1 31.4 24.1 16.5 15 15 15
y8-98 (1+) 21.8 8.4 4.5 23.6 9.1 5.2 32.1 12.4 6.9 15 15 15
y7 (1+) 33.8 35 12.2 35.1 52.6 13.1 48.7 63.2 17.9 15 15 15
y6 (1+) 7.6 4.6 3.1 14.8 5.3 5.4 16.6 7 6.2 15 15 15
sum 9.8 2.4 3.3 13.8 3.6 5.9 16.9 4.3 6.8 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-5707 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAPK8
Peptide Modified Sequence
TAGTSFMMTPY[+79.966331]VVTR
Modification Type
Phospho (Y)
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
175
Peptide End
189
CPTAC ID
non-CPTAC-5707
Peptide Molecular Mass
1,740.7616
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
N/A
Matrix
Cell Lysate
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Thermo Quantiva
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Dionex Ultimate 3000 RSLCnano
Column Packing
Pepmap 100
Column Dimensions
75um x 25cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 11.5 14.3 7.2 10.5 17.1 7.4 15.6 22.3 10.3 15 15 15
y9 (1+) 9.7 8.7 4.3 14.6 13.2 4.2 17.5 15.8 6 15 15 15
y8 (1+) 8.9 10 6.8 18.1 12.1 7.3 20.2 15.7 10 15 15 15
y7 (1+) 11.1 7.6 3.4 15.7 9 3.5 19.2 11.8 4.9 15 15 15
y6 (1+) 5.7 5 3.7 8.7 6.6 4 10.4 8.3 5.4 15 15 15
sum 3.9 4.5 2.4 7.6 5.7 2.9 8.5 7.3 3.8 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-889 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAPK8
Peptide Sequence
EVMDLEER
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
359
Peptide End
366
CPTAC ID
CPTAC-889
Peptide Molecular Mass
1,019.4594
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 9.3 4.7 4.6 8.3 5.3 4.7 12.5 7.1 6.6 15 15 15
y4 (1+) 4.9 2.9 2.4 5.6 3 2 7.4 4.2 3.1 15 15 15
y5 (1+) 6.4 3.1 1.9 5.6 3.7 2.2 8.5 4.8 2.9 15 15 15
y6 (1+) 5.8 2.4 2.6 6.2 2.8 2.9 8.5 3.7 3.9 15 15 15
sum 2.3 1.9 1.2 3 2.1 1.9 3.8 2.8 2.2 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-890 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAPK8
Peptide Sequence
NIIGLLNVFTPQK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
84
Peptide End
96
CPTAC ID
CPTAC-890
Peptide Molecular Mass
1,455.8450
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 18.9 5.6 3.4 20.1 7 3.4 27.6 9 4.8 15 15 15
y4 (1+) 26.3 13.1 7.1 26.8 14.3 6.8 37.5 19.4 9.8 15 15 15
y5 (1+) 31.2 9.6 7.6 29.5 10.6 10 42.9 14.3 12.6 15 15 15
y6 (1+) 22.7 15.1 14.9 26.6 13.6 17.5 35 20.3 23 15 15 15
y7 (1+) 26.5 7.3 10.7 26.6 11.6 11.1 37.5 13.7 15.4 15 15 15
y8 (1+) 33.3 12.5 8.8 30.3 15 8.8 45 19.5 12.4 15 15 15
y10 (1+) 16.3 4.1 6.2 12.4 10 9.5 20.5 10.8 11.3 15 15 15
y11 (1+) 26.5 11.9 8.1 31.6 9.6 9.8 41.2 15.3 12.7 15 15 15
b4 (1+) 30.7 13.8 8 31.6 14 8.3 44.1 19.7 11.5 15 15 15
b3 (1+) 25.7 8.9 15 29.3 13.3 15.1 39 16 21.3 15 15 15
sum 8.2 3 2.1 9 3.3 1.9 12.2 4.5 2.8 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-891 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAPK8
Peptide Modified Sequence
TAGTSFMMTPY[+80.0]VVTR
Modification Type
Phospho (Y)
Protein - Site of Modification
185
Peptide - Site of Modification
11
Peptide Start
175
Peptide End
189
CPTAC ID
CPTAC-891
Peptide Molecular Mass
1,740.7616
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 28 14.6 17.7 33.6 13.5 16.8 43.7 19.9 24.4 15 15 15
y6 (1+) 18.1 6.3 6.5 18.1 6.6 5.1 25.6 9.1 8.3 15 15 15
y7 (1+) 17.8 11 4.4 18.6 9.9 6 25.7 14.8 7.4 15 15 15
y8 (1+) 21.4 11.9 6.5 18.6 12.4 6 28.4 17.2 8.8 15 15 15
y9 (1+) 15.5 7.7 8.2 16.9 6.9 6.8 22.9 10.3 10.7 15 15 15
y11 (1+) 55.4 10 14.3 56.6 15.4 9.3 79.2 18.4 17.1 15 15 15
b3 (1+) 34.7 27 15.8 34.7 29 18.9 49.1 39.6 24.6 15 15 15
sum 10.3 3.7 1.5 10.8 5 2.7 14.9 6.2 3.1 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-892 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAPK8
Peptide Sequence
TAGTSFMMTPYVVTR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
175
Peptide End
189
CPTAC ID
CPTAC-892
Peptide Molecular Mass
1,660.7953
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 17 12.3 13.1 22.6 11.7 15.3 28.3 17 20.1 15 15 15
y6 (1+) 6.1 4.1 3.9 7.1 4.6 4.7 9.4 6.2 6.1 15 15 15
y7 (1+) 7.4 6.1 7.1 11.9 5.4 9.1 14 8.1 11.5 15 15 15
y8 (1+) 7.5 3.8 4.3 9.9 3.5 4.1 12.4 5.2 5.9 15 15 15
y9 (1+) 7.4 6.5 2.8 6.7 6.2 3.4 10 9 4.4 15 15 15
y10 (1+) 9.4 6.4 5.8 14.5 6.7 6.3 17.3 9.3 8.6 15 15 15
y11 (1+) 7.9 10.1 5 12.7 9.4 5.5 15 13.8 7.4 15 15 15
b3 (1+) 15.2 11.8 10.3 16 13.2 14.9 22.1 17.7 18.1 15 15 15
sum 3.8 2 2.5 6.5 1.9 2.9 7.5 2.8 3.8 15 15 15


Additional Resources and Comments