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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

IL16

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
IL16 N/A
Sequence Length (AA) Molecular Weight (Da)
1332 141752
Protein Name
Pro-interleukin-16
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MESHSRAGKS RKSAKFRSIS RSLMLCNAKT SDDGSSPDEK YPDPFEISLA
60 70 80 90 100
QGKEGIFHSS VQLADTSEAG PSSVPDLALA SEAAQLQAAG NDRGKTCRRI
110 120 130 140 150
FFMKESSTAS SREKPGKLEA QSSNFLFPKA CHQRARSNST SVNPYCTREI
160 170 180 190 200
DFPMTKKSAA PTDRQPYSLC SNRKSLSQQL DCPAGKAAGT SRPTRSLSTA
210 220 230 240 250
QLVQPSGGLQ ASVISNIVLM KGQAKGLGFS IVGGKDSIYG PIGIYVKTIF
260 270 280 290 300
AGGAAAADGR LQEGDEILEL NGESMAGLTH QDALQKFKQA KKGLLTLTVR
310 320 330 340 350
TRLTAPPSLC SHLSPPLCRS LSSSTCITKD SSSFALESPS APISTAKPNY
360 370 380 390 400
RIMVEVSLQK EAGVGLGIGL CSVPYFQCIS GIFVHTLSPG SVAHLDGRLR
410 420 430 440 450
CGDEIVEISD SPVHCLTLNE VYTILSHCDP GPVPIIVSRH PDPQVSEQQL
460 470 480 490 500
KEAVAQAVEN TKFGKERHQW SLEGVKRLES SWHGRPTLEK EREKNSAPPH
510 520 530 540 550
RRAQKVMIRS SSDSSYMSGS PGGSPGSGSA EKPSSDVDIS THSPSLPLAR
560 570 580 590 600
EPVVLSIASS RLPQESPPLP ESRDSHPPLR LKKSFEILVR KPMSSKPKPP
610 620 630 640 650
PRKYFKSDSD PQKSLEEREN SSCSSGHTPP TCGQEARELL PLLLPQEDTA
660 670 680 690 700
GRSPSASAGC PGPGIGPQTK SSTEGEPGWR RASPVTQTSP IKHPLLKRQA
710 720 730 740 750
RMDYSFDTTA EDPWVRISDC IKNLFSPIMS ENHGHMPLQP NASLNEEEGT
760 770 780 790 800
QGHPDGTPPK LDTANGTPKV YKSADSSTVK KGPPVAPKPA WFRQSLKGLR
810 820 830 840 850
NRASDPRGLP DPALSTQPAP ASREHLGSHI RASSSSSSIR QRISSFETFG
860 870 880 890 900
SSQLPDKGAQ RLSLQPSSGE AAKPLGKHEE GRFSGLLGRG AAPTLVPQQP
910 920 930 940 950
EQVLSSGSPA ASEARDPGVS ESPPPGRQPN QKTLPPGPDP LLRLLSTQAE
960 970 980 990 1000
ESQGPVLKMP SQRARSFPLT RSQSCETKLL DEKTSKLYSI SSQVSSAVMK
1010 1020 1030 1040 1050
SLLCLPSSIS CAQTPCIPKE GASPTSSSNE DSAANGSAET SALDTGFSLN
1060 1070 1080 1090 1100
LSELREYTEG LTEAKEDDDG DHSSLQSGQS VISLLSSEEL KKLIEEVKVL
1110 1120 1130 1140 1150
DEATLKQLDG IHVTILHKEE GAGLGFSLAG GADLENKVIT VHRVFPNGLA
1160 1170 1180 1190 1200
SQEGTIQKGN EVLSINGKSL KGTTHHDALA ILRQAREPRQ AVIVTRKLTP
1210 1220 1230 1240 1250
EAMPDLNSST DSAASASAAS DVSVESTAEA TVCTVTLEKM SAGLGFSLEG
1260 1270 1280 1290 1300
GKGSLHGDKP LTINRIFKGA ASEQSETVQP GDEILQLGGT AMQGLTRFEA
1310 1320 1330 1332
WNIIKALPDG PVTIVIRRKS LQSKETTAAG DS

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-926 Collapse assay details

Data source: Panorama

Official Gene Symbol
IL16
Peptide Modified Sequence
LLS[+80.0]TQAEESQGPVLK
Modification Type
Phospho (ST)
Protein - Site of Modification
946
Peptide - Site of Modification
3
Peptide Start
944
Peptide End
958
CPTAC ID
CPTAC-926
Peptide Molecular Mass
1,678.8179
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
IMAC
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTrap 5500 (Sciex)
Internal Standard
Peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
nanoLC-2D (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
75 µm x 15cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 36.4 23.1 27.7 58.1 31.8 32.3 68.6 39.3 42.6 15 24 15
y5 (1+) 36.9 18.5 20.1 29.9 19.1 21.5 47.5 26.6 29.4 15 24 15
y7 (1+) 55.3 13.5 13.2 87.3 20 24.9 103.3 24.1 28.2 15 24 15
sum 36.5 11.3 11.2 55.7 14.9 14.4 66.6 18.7 18.2 15 24 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for CPTAC-6050 Collapse assay details

Data source: Panorama

Official Gene Symbol
IL16
Peptide Sequence
LLSTQAEESQGPVLK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
944
Peptide End
958
CPTAC ID
CPTAC-6050
Peptide Molecular Mass
1,598.8516
Species
Human
Assay Type
Enrichment MRM
Enrichment Method
IMAC
Matrix
FFPE lung tumor tissue lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Whiteaker JR, Zhao L, Schoenherr RM, Huang D, Lundeen RA, Voytovich U, Kennedy JJ, Ivey RG, Lin C, Murillo OD, Lorentzen TD, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Reading JJ, Perry CD, Richardson CW, Garcia-Buntley SS, Bocik W, Hewitt SM, Chowdhury S, Vandermeer J, Smith SD, Gopal AK, Ramchurren N, Fling SP, Wang P, Paulovich AG. A multiplexed assay for quantifying immunomodulatory proteins supports correlative studies in immunotherapy clinical trials. Front Oncol. 2023 May 2;13:1168710. doi: 10.3389/fonc.2023.1168710. PMID: 37205196; PMCID: PMC10185886.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTRAP 5500
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Eksigent nanoLC 425
Column Packing
3 µm ChromXP C18EP, 120 Å
Column Dimensions
150 x 0.075 mm
Flow Rate
0.3 µL/min

Chromatograms

Data source: Panorama

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view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 23.9 15.7 19.9 22.3 16 20.6 32.7 22.4 28.6 15 15 15
y11 (1+) 36.7 15.3 31.5 44.9 15.1 32.5 58 21.5 45.3 15 15 15
y4 (1+) 30.4 11.3 19.1 30.3 11.5 24.4 42.9 16.1 31 15 15 15
y5 (1+) 23.3 10.8 24 22.1 11.2 24.9 32.1 15.6 34.6 15 15 15
y7 (1+) 28.9 20.8 22.8 30 23.7 21.9 41.7 31.5 31.6 15 15 15
y8 (1+) 23.7 10.7 20.8 24.6 10.6 19.2 34.2 15.1 28.3 15 15 15
y9 (1+) 18.6 10.6 21.9 25.7 13.5 22.6 31.7 17.2 31.5 15 15 15
sum 20.8 7.7 19.2 19.7 8.5 20 28.6 11.5 27.7 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments