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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
RCSD1 CAPZIP
Sequence Length (AA) Molecular Weight (Da)
416 44504
Protein Name
CapZ-interacting protein
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MEERPAETNA NVDNSASPSV AQLAGRFREQ AAAAKETPAS KPTRRKPPCS
60 70 80 90 100
LPLFPPKVDL GQNGEEKSPP NASHPPKFKV KSSPLIEKLQ ANLTFDPAAL
110 120 130 140 150
LPGASPKSPG LKAMVSPFHS PPSTPSSPGV RSRPSEAEEV PVSFDQPPEG
160 170 180 190 200
SHLPCYNKVR TRGSIKRRPP SRRFRRSQSD CGELGDFRAV ESSQQNGAKE
210 220 230 240 250
EDGDEVLPSK SKAPGSPLSS EGAAGEGVRT LGPAEKPPLR RSPSRTEKQE
260 270 280 290 300
EDRATEEAKN GEKARRSSEE VDGQHPAQEE VPESPQTSGP EAENRCGSPR
310 320 330 340 350
EEKPAGEEAE MEKATEVKGE RVQNEEVGPE HDSQETKKLE EGAAVKETPH
360 370 380 390 400
SPPGGVKGGD VPKQEKGKEK QQEGAVLEPG CSPQTGPAQL ETSSEVQSEP
410 416
AVPKPEDDTP VQDTKM

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-998 Collapse assay details

Data source: Panorama

Official Gene Symbol
RCSD1
Peptide Modified Sequence
SQS[+80.0]DC[+57.0]GELGDFR
Modification Type
Phospho (ST), Carbamidomethyl Cysteine
Protein - Site of Modification
179, 181
Peptide - Site of Modification
3, 5
Peptide Start
177
Peptide End
188
CPTAC ID
CPTAC-998
Peptide Molecular Mass
1,449.5232
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
IMAC
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTrap 5500 (Sciex)
Internal Standard
synthetic peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
nanoLC-2D (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
75 µm x 15cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 20.8 5.6 5.6 29.1 6.4 7.2 35.8 8.5 9.1 15 24 15
y7 (1+) 32.8 6.2 6.3 35.6 6.3 7.8 48.4 8.8 10 15 24 15
y8 (1+) 25.1 6.2 9.5 27.7 6.1 10.4 37.4 8.7 14.1 15 24 15
sum 20.8 5.1 6.2 26.6 5.4 7.7 33.8 7.4 9.9 15 24 15


Additional Resources and Comments


Assay Details for non-CPTAC-3907 Collapse assay details

Data source: Panorama

Official Gene Symbol
Rcsd1
Peptide Sequence
AEGTTIAEPDTK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
357
Peptide End
368
CPTAC ID
non-CPTAC-3907
Peptide Molecular Mass
1,231.5932
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 12.5 7.5 7.9 12.9 9.3 8.1 18 11.9 11.3 15 15 15
y2 (1+) 14.4 7.9 6.3 11.6 8 8.8 18.5 11.2 10.8 15 15 15
y4 (1+) 11.9 13.3 7.7 16 13.8 11.9 19.9 19.2 14.2 15 15 15
y6 (1+) 7.8 9.4 7.1 10.9 10.4 8.2 13.4 14 10.8 15 15 15
y8 (1+) 19.7 11.1 7.2 18.2 17.7 7.2 26.8 20.9 10.2 15 15 15
sum 5.9 4.8 3.6 7.4 7.4 6.2 9.5 8.8 7.2 15 15 15


Additional Resources and Comments