Show Nav →
Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
MST1 D3F15S2, DNF15S2, HGFL
Sequence Length (AA) Molecular Weight (Da)
711 80320
Protein Name
Hepatocyte growth factor-like protein
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MGWLPLLLLL TQCLGVPGQR SPLNDFQVLR GTELQHLLHA VVPGPWQEDV
60 70 80 90 100
ADAEECAGRC GPLMDCRAFH YNVSSHGCQL LPWTQHSPHT RLRRSGRCDL
110 120 130 140 150
FQKKDYVRTC IMNNGVGYRG TMATTVGGLP CQAWSHKFPN DHKYTPTLRN
160 170 180 190 200
GLEENFCRNP DGDPGGPWCY TTDPAVRFQS CGIKSCREAA CVWCNGEEYR
210 220 230 240 250
GAVDRTESGR ECQRWDLQHP HQHPFEPGKF LDQGLDDNYC RNPDGSERPW
260 270 280 290 300
CYTTDPQIER EFCDLPRCGS EAQPRQEATT VSCFRGKGEG YRGTANTTTA
310 320 330 340 350
GVPCQRWDAQ IPHQHRFTPE KYACKDLREN FCRNPDGSEA PWCFTLRPGM
360 370 380 390 400
RAAFCYQIRR CTDDVRPQDC YHGAGEQYRG TVSKTRKGVQ CQRWSAETPH
410 420 430 440 450
KPQFTFTSEP HAQLEENFCR NPDGDSHGPW CYTMDPRTPF DYCALRRCAD
460 470 480 490 500
DQPPSILDPP DQVQFEKCGK RVDRLDQRRS KLRVVGGHPG NSPWTVSLRN
510 520 530 540 550
RQGQHFCGGS LVKEQWILTA RQCFSSCHMP LTGYEVWLGT LFQNPQHGEP
560 570 580 590 600
SLQRVPVAKM VCGPSGSQLV LLKLERSVTL NQRVALICLP PEWYVVPPGT
610 620 630 640 650
KCEIAGWGET KGTGNDTVLN VALLNVISNQ ECNIKHRGRV RESEMCTEGL
660 670 680 690 700
LAPVGACEGD YGGPLACFTH NCWVLEGIII PNRVCARSRW PAVFTRVSVF
710 711
VDWIHKVMRL G

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

loading

Loader

Assay Details for CPTAC-6061 Collapse assay details

Data source: Panorama

Official Gene Symbol
MST1
Peptide Sequence
VVGGHPGNSPWTVSLR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
484
Peptide End
499
CPTAC ID
CPTAC-6061
Peptide Molecular Mass
1,661.8638
Species
Human
Assay Type
Enrichment MRM
Enrichment Method
N/A
Matrix
FFPE lung tumor tissue lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Whiteaker JR, Zhao L, Schoenherr RM, Huang D, Lundeen RA, Voytovich U, Kennedy JJ, Ivey RG, Lin C, Murillo OD, Lorentzen TD, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Reading JJ, Perry CD, Richardson CW, Garcia-Buntley SS, Bocik W, Hewitt SM, Chowdhury S, Vandermeer J, Smith SD, Gopal AK, Ramchurren N, Fling SP, Wang P, Paulovich AG. A multiplexed assay for quantifying immunomodulatory proteins supports correlative studies in immunotherapy clinical trials. Front Oncol. 2023 May 2;13:1168710. doi: 10.3389/fonc.2023.1168710. PMID: 37205196; PMCID: PMC10185886.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTRAP 5500
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Eksigent nanoLC 425
Column Packing
3 µm ChromXP C18EP, 120 Å
Column Dimensions
150 x 0.075 mm
Flow Rate
0.3 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 27.1 16.7 18.4 26.2 19.6 31.5 37.7 25.7 36.5 15 15 15
y4 (1+) 18.2 12.1 19 18.7 12.7 27.2 26.1 17.5 33.2 15 15 15
y5 (1+) 19.5 8.3 21 19.1 11.9 23.7 27.3 14.5 31.7 15 15 15
y6 (1+) 13.7 13.5 26 18.6 17.3 27.3 23.1 21.9 37.7 15 15 15
y7 (1+) 19.1 9.1 35 20.5 11 37.4 28 14.3 51.2 15 15 15
y7 (2+) 20.9 12.4 24.2 19.8 12.4 29.9 28.8 17.5 38.5 15 15 15
sum 16 7.9 14.9 15.5 9.5 22.2 22.3 12.4 26.7 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-2672 Collapse assay details

Data source: Panorama

Official Gene Symbol
MST1
Peptide Sequence
SPLNDFQVLR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
21
Peptide End
30
CPTAC ID
non-CPTAC-2672
Peptide Molecular Mass
1,187.6299
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 6.6 6.5 10.9 13.7 9.5 10.6 15.2 11.5 15.2 15 15 15
y5 (1+) 13.2 6.7 9.1 16.1 12.2 11.7 20.8 13.9 14.8 15 15 15
y3 (1+) 14.7 6.3 10.3 15.3 8.8 13.2 21.2 10.8 16.7 15 15 15
y9 (2+) 17.6 6.3 7.4 20 9.2 11.1 26.6 11.2 13.3 15 15 15
y7 (1+) 9.2 6.2 6.4 11.3 8.4 9.4 14.6 10.4 11.4 15 15 15
sum 8.2 4 4 12.2 7.9 8.5 14.7 8.9 9.4 15 15 15


Additional Resources and Comments