MST1, CPTAC-2672 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
MST1	D3F15S2, DNF15S2, HGFL

Sequence Length (AA)	Molecular Weight (Da)
711	80320

Protein Name
Hepatocyte growth factor-like protein

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MGWLPLLLLL	TQCLGVPGQR	SPLNDFQVLR	GTELQHLLHA	VVPGPWQEDV
60	70	80	90	100
ADAEECAGRC	GPLMDCRAFH	YNVSSHGCQL	LPWTQHSPHT	RLRRSGRCDL
110	120	130	140	150
FQKKDYVRTC	IMNNGVGYRG	TMATTVGGLP	CQAWSHKFPN	DHKYTPTLRN
160	170	180	190	200
GLEENFCRNP	DGDPGGPWCY	TTDPAVRFQS	CGIKSCREAA	CVWCNGEEYR
210	220	230	240	250
GAVDRTESGR	ECQRWDLQHP	HQHPFEPGKF	LDQGLDDNYC	RNPDGSERPW
260	270	280	290	300
CYTTDPQIER	EFCDLPRCGS	EAQPRQEATT	VSCFRGKGEG	YRGTANTTTA
310	320	330	340	350
GVPCQRWDAQ	IPHQHRFTPE	KYACKDLREN	FCRNPDGSEA	PWCFTLRPGM
360	370	380	390	400
RAAFCYQIRR	CTDDVRPQDC	YHGAGEQYRG	TVSKTRKGVQ	CQRWSAETPH
410	420	430	440	450
KPQFTFTSEP	HAQLEENFCR	NPDGDSHGPW	CYTMDPRTPF	DYCALRRCAD
460	470	480	490	500
DQPPSILDPP	DQVQFEKCGK	RVDRLDQRRS	KLRVVGGHPG	NSPWTVSLRN
510	520	530	540	550
RQGQHFCGGS	LVKEQWILTA	RQCFSSCHMP	LTGYEVWLGT	LFQNPQHGEP
560	570	580	590	600
SLQRVPVAKM	VCGPSGSQLV	LLKLERSVTL	NQRVALICLP	PEWYVVPPGT
610	620	630	640	650
KCEIAGWGET	KGTGNDTVLN	VALLNVISNQ	ECNIKHRGRV	RESEMCTEGL
660	670	680	690	700
LAPVGACEGD	YGGPLACFTH	NCWVLEGIII	PNRVCARSRW	PAVFTRVSVF
710	711
VDWIHKVMRL	G

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node

to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-6061 Collapse assay details

Data source: Panorama

Official Gene Symbol: MST1
Peptide Sequence: VVGGHPGNSPWTVSLR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 484
Peptide End: 499

CPTAC ID: CPTAC-6061
Peptide Molecular Mass: 1,661.8638
Species: Human
Assay Type: Enrichment MRM
Enrichment Method: N/A
Matrix: FFPE lung tumor tissue lysate pool
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Whiteaker JR, Zhao L, Schoenherr RM, Huang D, Lundeen RA, Voytovich U, Kennedy JJ, Ivey RG, Lin C, Murillo OD, Lorentzen TD, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Reading JJ, Perry CD, Richardson CW, Garcia-Buntley SS, Bocik W, Hewitt SM, Chowdhury S, Vandermeer J, Smith SD, Gopal AK, Ramchurren N, Fling SP, Wang P, Paulovich AG. A multiplexed assay for quantifying immunomodulatory proteins supports correlative studies in immunotherapy clinical trials. Front Oncol. 2023 May 2;13:1168710. doi: 10.3389/fonc.2023.1168710. PMID: 37205196; PMCID: PMC10185886.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: QTRAP 5500
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Eksigent nanoLC 425
Column Packing: 3 µm ChromXP C18EP, 120 Å
Column Dimensions: 150 x 0.075 mm
Flow Rate: 0.3 µL/min

Chromatograms

Data source: Panorama

view larger image

Response Curves

Data source: Panorama

view larger image

Retrieving Data

Repeatability

Data source: Panorama

view larger image

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y3 (1+)	27.1	16.7	18.4	26.2	19.6	31.5	37.7	25.7	36.5	15	15	15
y4 (1+)	18.2	12.1	19	18.7	12.7	27.2	26.1	17.5	33.2	15	15	15
y5 (1+)	19.5	8.3	21	19.1	11.9	23.7	27.3	14.5	31.7	15	15	15
y6 (1+)	13.7	13.5	26	18.6	17.3	27.3	23.1	21.9	37.7	15	15	15
y7 (1+)	19.1	9.1	35	20.5	11	37.4	28	14.3	51.2	15	15	15
y7 (2+)	20.9	12.4	24.2	19.8	12.4	29.9	28.8	17.5	38.5	15	15	15
sum	16	7.9	14.9	15.5	9.5	22.2	22.3	12.4	26.7	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for non-CPTAC-2672 Collapse assay details

Data source: Panorama

Official Gene Symbol: MST1
Peptide Sequence: SPLNDFQVLR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 21
Peptide End: 30

CPTAC ID: non-CPTAC-2672
Peptide Molecular Mass: 1,187.6299
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers