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Overview Data source: UniProt

Official Gene Symbol Other Aliases
F2 Cf2
Sequence Length (AA) Molecular Weight (Da)
618 70269
Protein Name
Prothrombin
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MSHVRGLGLP GCLALAALVS LVHSQHVFLA PQQALSLLQR VRRANSGFLE
60 70 80 90 100
ELRKGNLERE CVEEQCSYEE AFEALESPQD TDVFWAKYTV CDSVRKPRET
110 120 130 140 150
FMDCLEGRCA MDLGVNYLGT VNVTHTGIQC QLWRSRYPHK PEINSTTHPG
160 170 180 190 200
ADLKENFCRN PDSSTTGPWC YTTDPTVRRE ECSVPVCGQE GRTTVVMTPR
210 220 230 240 250
SGGSKDNLSP PLGQCLTERG RLYQGNLAVT TLGSPCLPWN SLPAKTLSKY
260 270 280 290 300
QDFDPEVKLV ENFCRNPDWD EEGAWCYVAG QPGDFEYCNL NYCEEAVGEE
310 320 330 340 350
NYDVDESIAG RTTDAEFHTF FNEKTFGLGE ADCGLRPLFE KKSLKDTTEK
360 370 380 390 400
ELLDSYIDGR IVEGWDAEKG IAPWQVMLFR KSPQELLCGA SLISDRWVLT
410 420 430 440 450
AAHCILYPPW DKNFTENDLL VRIGKHSRTR YERNVEKISM LEKIYVHPRY
460 470 480 490 500
NWRENLDRDI ALLKLKKPVP FSDYIHPVCL PDKQTVTSLL RAGYKGRVTG
510 520 530 540 550
WGNLRETWTT NINEIQPSVL QVVNLPIVER PVCKASTRIR ITDNMFCAGF
560 570 580 590 600
KVNDTKRGDA CEGDSGGPFV MKSPFNNRWY QMGIVSWGEG CDRKGKYGFY
610 618
THVFRLKRWI QKVIDQFG

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-5620 Collapse assay details

Data source: Panorama

Official Gene Symbol
F2
Peptide Sequence
TTDAEFHTFFNEK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
312
Peptide End
324
CPTAC ID
non-CPTAC-5620
Peptide Molecular Mass
1,585.7049
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b9 (2+) 12.6 9.7 17.1 16.5 8.6 23.4 20.8 13 29 15 15 15
b2 (1+) 2.8 2.5 3.4 5.1 6.1 4.3 5.8 6.6 5.5 15 15 15
y11 (2+) 3.2 2.9 3.4 5.3 8.5 3.9 6.2 9 5.2 15 15 15
y12 (2+) 5.8 2.4 2.9 9.9 7.3 4.1 11.5 7.7 5 15 15 15
y6 (1+) 6.4 3.8 3.1 8.6 7.4 4.2 10.7 8.3 5.2 15 15 15
sum 2.2 1.5 1.8 4.4 6.5 3.2 4.9 6.7 3.7 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-3432 Collapse assay details

Data source: Panorama

Official Gene Symbol
F2
Peptide Sequence
YGFYTHVFR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
597
Peptide End
605
CPTAC ID
non-CPTAC-3432
Peptide Molecular Mass
1,188.5716
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (2+) 7.5 6.6 8.5 22.3 7.3 10.1 23.5 9.8 13.2 15 14 15
y7 (2+) 4.2 9.5 6.2 14.8 11.8 7.1 15.4 15.1 9.4 15 14 15
y8 (2+) 8.7 5.3 5.4 16.2 10.4 6.7 18.4 11.7 8.6 15 14 15
y4 (1+) 8.2 7.4 7.5 10.8 10.5 6.9 13.6 12.8 10.2 15 14 15
y5 (1+) 4.8 6.3 5.9 9.1 9 6.4 10.3 11 8.7 15 14 15
sum 4.9 4.6 3.5 14.5 8.7 4.4 15.3 9.8 5.6 15 14 15

Additional Resources and Comments