Tek, CPTAC-3433 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
Tek	Hyk, Tie-2, Tie2

Sequence Length (AA)	Molecular Weight (Da)
1122	125701

Protein Name
Angiopoietin-1 receptor

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MDSLAGLVLC	GVSLLLYGVV	EGAMDLILIN	SLPLVSDAET	SLTCIASGWH
60	70	80	90	100
PHEPITIGRD	FEALMNQHQD	PLEVTQDVTR	EWAKKVVWKR	EKASKINGAY
110	120	130	140	150
FCEGRVRGQA	IRIRTMKMRQ	QASFLPATLT	MTVDRGDNVN	ISFKKVLIKE
160	170	180	190	200
EDAVIYKNGS	FIHSVPRHEV	PDILEVHLPH	AQPQDAGVYS	ARYIGGNLFT
210	220	230	240	250
SAFTRLIVRR	CEAQKWGPDC	SRPCTTCKNN	GVCHEDTGEC	ICPPGFMGRT
260	270	280	290	300
CEKACEPHTF	GRTCKERCSG	PEGCKSYVFC	LPDPYGCSCA	TGWRGLQCNE
310	320	330	340	350
ACPSGYYGPD	CKLRCHCTNE	EICDRFQGCL	CSQGWQGLQC	EKEGRPRMTP
360	370	380	390	400
QIEDLPDHIE	VNSGKFNPIC	KASGWPLPTS	EEMTLVKPDG	TVLQPNDFNY
410	420	430	440	450
TDRFSVAIFT	VNRVLPPDSG	VWVCSVNTVA	GMVEKPFNIS	VKVLPEPLHA
460	470	480	490	500
PNVIDTGHNF	AIINISSEPY	FGDGPIKSKK	LFYKPVNQAW	KYIEVTNEIF
510	520	530	540	550
TLNYLEPRTD	YELCVQLARP	GEGGEGHPGP	VRRFTTASIG	LPPPRGLSLL
560	570	580	590	600
PKSQTALNLT	WQPIFTNSED	EFYVEVERRS	LQTTSDQQNI	KVPGNLTSVL
610	620	630	640	650
LSNLVPREQY	TVRARVNTKA	QGEWSEELRA	WTLSDILPPQ	PENIKISNIT
660	670	680	690	700
DSTAMVSWTI	VDGYSISSII	IRYKVQGKNE	DQHIDVKIKN	ATVTQYQLKG
710	720	730	740	750
LEPETTYHVD	IFAENNIGSS	NPAFSHELRT	LPHSPASADL	GGGKMLLIAI
760	770	780	790	800
LGSAGMTCIT	VLLAFLIMLQ	LKRANVQRRM	AQAFQNREEP	AVQFNSGTLA
810	820	830	840	850
LNRKAKNNPD	PTIYPVLDWN	DIKFQDVIGE	GNFGQVLKAR	IKKDGLRMDA
860	870	880	890	900
AIKRMKEYAS	KDDHRDFAGE	LEVLCKLGHH	PNIINLLGAC	EHRGYLYLAI
910	920	930	940	950
EYAPHGNLLD	FLRKSRVLET	DPAFAIANST	ASTLSSQQLL	HFAADVARGM
960	970	980	990	1000
DYLSQKQFIH	RDLAARNILV	GENYIAKIAD	FGLSRGQEVY	VKKTMGRLPV
1010	1020	1030	1040	1050
RWMAIESLNY	SVYTTNSDVW	SYGVLLWEIV	SLGGTPYCGM	TCAELYEKLP
1060	1070	1080	1090	1100
QGYRLEKPLN	CDDEVYDLMR	QCWREKPYER	PSFAQILVSL	NRMLEERKTY
1110	1120	1122
VNTTLYEKFT	YAGIDCSAEE	AA

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-3433 Collapse assay details

Data source: Panorama

Official Gene Symbol: Tek
Peptide Sequence: YIGGNLFTSAFTR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 193
Peptide End: 205

CPTAC ID: non-CPTAC-3433
Peptide Molecular Mass: 1,445.7303
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b2 (1+)	12.3	2.3	4.4	41.9	4.7	4.9	43.7	5.2	6.6	15	15	15
y6 (1+)	9.3	4.6	2.6	14.8	5.9	3.8	17.5	7.5	4.6	15	15	15
y7 (1+)	5.8	4.2	2.4	10.4	6.5	2.6	11.9	7.7	3.5	15	15	15
y8 (1+)	6.9	4.8	3.2	12.8	6.6	6.1	14.5	8.2	6.9	15	15	15
y11 (1+)	5.2	4.3	2.1	16.7	7.2	3.8	17.5	8.4	4.3	15	15	15
sum	3.9	1.3	1.3	14	4.1	2.9	14.5	4.3	3.2	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for CPTAC-2892 Collapse assay details

Data source: Panorama

Official Gene Symbol: TEK
Peptide Sequence: FQDVIGEGNFGQVLK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 826
Peptide End: 840

CPTAC ID: CPTAC-2892
Peptide Molecular Mass: 1,649.8413
Species: Homo Sapiens
Assay Type: Direct MRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend